Study on vernalization mechanism of Brassica crops using newly developed Intromap, a bioinformatic tool to identify alien genomic introgression regions
| Project/Area Number |
18H02173
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 39010:Science in plant genetics and breeding-related
|
|---|
| Research Institution | Niigata University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
深井 英吾 新潟大学, 自然科学系, 准教授 (00570657)
柿崎 智博 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門, 上級研究員 (30547229)
藤本 龍 神戸大学, 農学研究科, 准教授 (60620375)
板橋 悦子 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門, 主任研究員 (70783273)
|
|---|
| Project Period (FY) |
2018-04-01 – 2021-03-31
|
| Project Status |
Completed (Fiscal Year 2020)
|
| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
|
|---|
| Keywords | 春化 / Brassica / エピジェネティクス / 遺伝子発現 / アブラナ科作物 / RNA-seq / キャベツ / ハクサイ / イントログレッション / RNAseq / エピゲノム / 遺伝育種 |
|---|
| Outline of Final Research Achievements |
In order to elucidate the vernalization mechanism of the genus Brassica, we did the following studies. We produced C-genome chromosome addition lines using BoFLC2-introgressed B. rapa as a recurrent parent and a cabbage as a c-genome chromosome donor, and characterized those lines with respect to the parental cultivars. In Chinese cabbage, the epigenetic regulation of gene expression at the FLC locus was clarified by RNA-seq, lncRNA analysis, and chromatin immunoprecipitation analysis. In addition, transcriptome analysis was performed on the leaves of the cabbage DH strain with/without low temperature treatment. QTL analysis was performed to determine vernalization characteristics of rapeseed cultivars using F2 populations derived from the crossing between seed vernalization typed rapeseed x green plant vernalization typed rapeseed.
|
| Academic Significance and Societal Importance of the Research Achievements |
B. oleraceaでは, BoFLC1について、低温および葉齢依存的な発現パターンに明確な品種間差があることが明らかになった。BoFLC1と晩抽性との関連性が明らかになれば, 晩抽性品種の育種の際にマーカー遺伝子として利用できる。B. rapaでは, FLCパラログの発現量と春化要求性との関連性を明らかにでき, BrFLC座のエピジェネティックな転写制御について新知見を得ることができた。農作物には, ムギ類, エンドウ, ソラマメ(種子春化), タマネギ, セロリ, ニンジン(緑体春化)など春化特性を持つものも多く、本研究で明らかにしたブラシカ属の知見が他作物での研究の参考となる。
|
Report
(3 results)
Research Products
(23 results)
-
[Journal Article] The histone modification H3 lysine 27 tri-methylation has conserved gene regulatory roles in the triplicated genome of Brassica rapa L.2019
Author(s)
Akter A, Takahashi S, Deng W, Shea DJ, Itabashi E, Shimizu M, Miyaji N, Osabe K, Nishida N, Suzuki Y, Helliwell CA, Seki M, Peacock WJ, Dennis ES, Fujimoto R.
-
Journal Title
DNA Research
Volume: 26
Issue: 5
Pages: 433-443
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] The role of FRIGIDA and FLOWERING LOCUS C genes in flowering time of Brassica rapa leafy vegetables.2019
Author(s)
Satoko Takada, Ayasha Akter, Etsuko Itabashi, Namiko Nishida, Daniel J Shea, Naomi Miyaji, Hasan Mehraj, Kenji Osabe, Motoki Shimizu, Takeshi Takasaki-Yasuda, Tomohiro Kakizaki, Keiichi Okazaki, Elizabeth S Dennis, Ryo Fujimoto
-
Journal Title
Scientific reports
Volume: 9(1)
Issue: 1
Pages: 13843-13843
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Long noncoding RNAs in Brassica rapa L. following vernalization.2019
Author(s)
Daniel J Shea, Namiko Nishida, Satoko Takada, Etsuko Itabashi, Satoshi Takahashi, Ayasha Akter, Naomi Miyaji, Kenji Osabe, Hasan Mehraj, Motoki Shimizu, Motoaki Seki, Tomohiro Kakizaki, Keiichi Okazaki, Elizabeth S Dennis, Ryo Fujimoto
-
Journal Title
Scientific reports
Volume: 9(1)
Issue: 1
Pages: 9302-9302
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
[Presentation] ハクサイにおける長鎖非コードRNAとエピジェネティクスとの関係性2019
Author(s)
西田菜美子, シェア ダニエル, 高橋聡史, 板橋悦子, アクタ アヤシャ, 宮路直実, メエラジ ハサン, 安田剛志, 柿崎智博, 関原明, 岡崎桂一, 藤本龍
Organizer
園芸学会令和元年度秋季大会
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
