Elucidation of the mechanism of radial oxygen loss barrier formation and verification of its contribution to the improvement of waterlogging tolerance of maize

• Project/Area Number: 18H02175
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 39010:Science in plant genetics and breeding-related
• Research Institution: Nagoya University
• Principal Investigator: Nakazono Mikio 名古屋大学, 生命農学研究科, 教授 (70282697)
• Co-Investigator(Kenkyū-buntansha): 間野 吉郎 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門, ユニット長 (20355126) ; 高溝 正 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門, 再雇用職員 (00355124)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2020: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2018: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: トウモロコシ / テオシント / 耐湿性遺伝子 / 酸素漏出バリア / マッピング

Outline of Final Research Achievements

Zea nicaraguensis, a wild relative of maize, exhibits higher waterlogging tolerance than maize, because it can form a radial oxygen loss (ROL) barrier that prevents oxygen leakage from the roots to waterlogged soil. In this study, we identified the regulatory gene for ROL barrier formation (designated as "ROL BARRIER FORMATION-1" (RBF1)) of Zea nicaraguensis by fine mapping, and confirmed that an ROL barrier was formed when RBF1 gene was transformed into maize. Root elongation under waterlogged deoxygenated conditions was larger in the near-isogenic line (NIL) containing the RBF1 locus than in maize inbred Mi29. RNA-Seq analysis suggested that RBF1 induces the expression of lignin and suberin biosynthetic genes to promote ROL barrier formation.

Academic Significance and Societal Importance of the Research Achievements

本研究によって、世界に先駆けてROLバリア形成制御遺伝子が同定できたことによって、作物の耐湿性付与に重要な形質であるROLバリア形成機構の全貌解明が可能となり、学術的意義は高いと言える。また、交配によってトウモロコシにニカラグアテオシントのROLバリア形成制御遺伝子を導入することで、高耐湿性トウモロコシ系統を作出する道筋を示すことができた。これらの研究結果は、野生遺伝資源由来の有用農業形質遺伝子を育種利用できることを実証するとともに、今後気候変動が深刻化しても安定した作物生産が可能となる方策を提案できる成果となった。

Research Products

• [Int'l Joint Research] 西オーストラリア大学(オーストラリア)
• [Int'l Joint Research] コペンハーゲン大学(デンマーク)
• [Int'l Joint Research] 西オーストラリア大学(オーストラリア)
• [Int'l Joint Research] コペンハーゲン大学(デンマーク)
• [Int'l Joint Research] 河南農業大学(中国)
• [Int'l Joint Research] 西オーストラリア大学(オーストラリア)
• [Int'l Joint Research] コペンハーゲン大学(デンマーク)
• [Journal Article] Genetic regulation of root traits for soil flooding tolerance in genus <i>Zea</i> (2021)
  • Author(s): Mano Yoshiro、Nakazono Mikio
  • Journal Title: Breeding Science Volume: 71 Issue: 1 Pages: 30-39
  • DOI: 10.1270/jsbbs.20117
  • NAID: 130007991318
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of root adaptive anatomical and morphological traits during low soil oxygen (2020)
  • Author(s): Pedersen Ole、Sauter Margret、Colmer Timothy David、Nakazono Mikio
  • Journal Title: New Phytologist Volume: 229 Issue: 1 Pages: 42-49
  • DOI: 10.1111/nph.16375
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lateral roots, in addition to adventitious roots, form a barrier to radial oxygen loss in Zea nicaraguensis and a chromosome segment introgression line in maize (2020)
  • Author(s): Pedersen Ole、Nakayama Yohei、Yasue Hiroki、Kurokawa Yusuke、Takahashi Hirokazu、Heidi Floytrup Anja、Omori Fumie、Mano Yoshiro、David Colmer Timothy、Nakazono Mikio
  • Journal Title: New Phytologist Volume: 229 Issue: 1 Pages: 94-105
  • DOI: 10.1111/nph.16452
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] QTLs for constitutive aerenchyma from Zea nicaraguensis improve tolerance of maize to root-zone oxygen deficiency (2019)
  • Author(s): Gong Fangping、Takahashi Hirokazu、Omori Fumie、Wang Wei、Mano Yoshiro、Nakazono Mikio
  • Journal Title: Journal of Experimental Botany Volume: 70 Issue: 21 Pages: 6475-6487
  • DOI: 10.1093/jxb/erz403
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Zea nicaraguensisの側根に形成される酸素漏出バリアの遺伝・生理学的解析 (2020)
  • Author(s): 中園幹生、中山洋平、安江広樹、黒川裕介、Ole Pedersen、Anja Heidi Floytrup、Timothy David Colmer、高橋宏和、大森史恵、間野吉郎
  • Organizer: 日本育種学会第138回講演会
• [Presentation] RNA-Seq解析によるZea nicaraguensisの酸素漏出バリア形成に関与する重要遺伝子の探索 (2020)
  • Author(s): 中山洋平、高橋宏和、Maurizio Camagna、佐藤 豊、豊田 敦、大森 史恵、間野吉郎、中園 幹生
  • Organizer: 日本育種学会第138回講演会
• [Presentation] Root traits that determine waterlogging resilience in rice and maize (2019)
  • Author(s): Nakazono Mikio
  • Organizer: Keystone Symposia Conference - Climate Change-Linked Stress Tolerance in Plants
  • Int'l Joint Research / Invited
• [Presentation] Roles of plant hormones in anatomical responses of roots to flooding stress (2019)
  • Author(s): Nakazono Mikio
  • Organizer: 23rd International Conference on Plant Growth Substances
  • Int'l Joint Research / Invited
• [Presentation] Zea nicaraguensis における酸素漏出バリア形成能の遺伝学的解析 (2018)
  • Author(s): 中山洋平, 渡邊宏太郎, 井出健斗, 黒川裕介, 髙橋宏和, 高橋秀和, Timothy D. Colmer, Ole Pedersen, 大森史恵, 間野吉郎, 中園幹生
  • Organizer: 第26回中部地区談話会