| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2021: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Outline of Final Research Achievements |
An in vitro pollen bioassay using antisense oligo (as-oligo) DNA was investigated to prove the function of the pollen S candidate gene (DUF247) in a wild species of barley (Hordeum bulbosum). The addition of as-oligo to the S1-haplotype pollen was suggested to break the incompatibility reaction in the S1 (self) pollen tube, but reproducibility could not be confirmed. Analysis of the S and Z locus synteny regions in Poaceae indicated that two different DUF247 homologs are closely linked and locate in the corresponding regions, respectively, and that they are classified into four different clades.
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