Molecular mechanism for breaking down self-incompatibility in buckwheat

• Project/Area Number: 18H02177
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 39010:Science in plant genetics and breeding-related
• Research Institution: Kyoto University
• Principal Investigator: Yasui Yasuo 京都大学, 農学研究科, 助教 (70293917)
• Co-Investigator(Kenkyū-buntansha): 松井 勝弘 国立研究開発法人農業・食品産業技術総合研究機構, 次世代作物開発研究センター, 上級研究員 (30343974) ; 中崎 鉄也 京都大学, 農学研究科, 教授 (60217693) ; 平川 英樹 公益財団法人かずさDNA研究所, ゲノム情報解析施設, 施設長 (80372746)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2018: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
• Keywords: ソバ / ゲノム解読 / S-ELF3 / FePG1 / 二花柱性 / 自家不和合性 / 自殖性 / 他殖性

Outline of Final Research Achievements

In this project, we tried to elucidate the molecular mechanism of the heterostylous self-incompatibility gene to enable self-fertilization of buckwheat. By developing a functionally deficient allele of S-ELF3 (S-ELF3-PS1), we have demonstrated that S-ELF3 regulates both style length and self-incompatibility of pistils in buckwheat. We also succeeded in identifying FePG1, which is considered to function under the control of S-ELF3.

Academic Significance and Societal Importance of the Research Achievements

本課題ではS-ELF3-PS1を開発し、ソバの自殖化を可能とした。また自殖性ソバを利用して、chromosomal pseudo-molecules (CPMs)の作成に成功した。これまでにソバではCPMsは作成されておらず、本課題だけでなく今後のソバ育種の貴重なツールとなる。さらに本課題で開発したTILLING集団はS遺伝子以外にももちろん利用可能である。本課題によってソバの遺伝育種学を大きく前進させることができたと考えている。

Research Products

• [Journal Article] Targeted amplicon sequencing + next-generation sequencing?based bulked segregant analysis identified genetic loci associated with preharvest sprouting tolerance in common buckwheat (Fagopyrum esculentum) (2021)
  • Author(s): Takeshima Ryoma、Ogiso-Tanaka Eri、Yasui Yasuo、Matsui Katsuhiro
  • Journal Title: BMC Plant Biology Volume: 21 Issue: 1 Pages: 18-18
  • DOI: 10.1186/s12870-020-02790-w
  • Peer Reviewed / Open Access
• [Journal Article] Genetic analysis of photoperiod sensitivity associated with difference in ecotype in common buckwheat (2020)
  • Author(s): Hara Takashi、Shima Taeko、Nagai Hiroya、Ohsawa Ryo
  • Journal Title: Breeding Science Volume: 70 Issue: 1 Pages: 101-111
  • DOI: 10.1270/jsbbs.19118
  • NAID: 130007809710
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed
• [Journal Article] History of the progressive development of genetic marker systems for common buckwheat (2020)
  • Author(s): Yasuo Yasui
  • Journal Title: Breeding Science Volume: 70 Issue: 1 Pages: 13-18
  • DOI: 10.1270/jsbbs.19075
  • NAID: 130007809718
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed / Open Access
• [Journal Article] Buckwheat heteromorphic self-incompatibility: genetics, genomics and application to breeding (2020)
  • Author(s): Katsuhiro Matsui, Yasuo Yasui
  • Journal Title: Breeding Science Volume: 70 Issue: 1 Pages: 32-38
  • DOI: 10.1270/jsbbs.19083
  • NAID: 130007809720
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed / Open Access
• [Journal Article] Gene flow signature in the S-allele region of cultivated buckwheat (2019)
  • Author(s): N. Mizuno, Y. Yasui
  • Journal Title: BMC Plant Biology Volume: 19 Issue: 1 Pages: 125-125
  • DOI: 10.1186/s12870-019-1730-1
  • NAID: 120006623974
  • Peer Reviewed / Open Access
• [Presentation] High genetic diversity within cultivated common buckwheat: implication of introgression from wild common buckwheat in China (2019)
  • Author(s): Yasuo Yasui
  • Organizer: 2019年第１回西南四省（自治区）遺伝学学会連合会
  • Int'l Joint Research / Invited
• [Presentation] ソバにおける異型花型自家不和合性遺伝子座のゲノム解析 (2019)
  • Author(s): 大田竜也, 相井城太郎, 上野まりこ, 大澤良, 齊藤大樹, 齊藤大樹, 白澤健太, 竹島亮馬, 中崎鉄也, 西村和紗, 原尚資, 原尚資, 平川英樹, FAWCETT J, 松井勝弘, 水野信之, 安井康夫
  • Organizer: 日本育種学会
• [Presentation] ソバ属植物におけるゲノムの進化 (2019)
  • Author(s): FAWCETT Jeffrey, 上野まりこ, 大澤良, 大田竜也, 齊藤大樹, 齊藤大樹, 白澤健太, 竹島亮馬, 中崎鉄也, 西村和紗, 原尚資, 原尚資, 平川英樹, 松井勝弘, 水野信之, 安井康夫
  • Organizer: 日本遺伝学会
• [Presentation] 研究者単位でのリファレンスゲノムの時代へ ーDeNovoMAGIC を用いたソバのリファレンス配列の作成とその利用についてー (2019)
  • Author(s): 安井康夫
  • Organizer: アグリゲノム産業研究会
  • Invited
• [Presentation] Identification of genes at the S-locus controlling heteromorphic self-incompatibility in buckwheat through genetic and genomic analyses (2018)
  • Author(s): Y. Yasui, N. Mizuno, J. Aii and T. Ota
  • Organizer: The Society for Molecular Biology & Evolution
  • Int'l Joint Research
• [Patent(Industrial Property Rights)] 機能欠損型転写因子様遺伝子及びこれを利用した自殖性ソバ属植物 (2021)
  • Inventor(s): 安井康夫
  • Industrial Property Rights Holder: 安井康夫
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2021-026492
  • Filing Date: 2021