QTL analysis of high-temperature tolerant wheat lines and development of selection markers

• Project/Area Number: 18H02179
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 39010:Science in plant genetics and breeding-related
• Research Institution: Tottori University
• Principal Investigator: TSUJIMOTO Hisashi 鳥取大学, 乾燥地研究センター, 教授 (50183075)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2020: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: パンコムギ / 高温耐性 / 乾燥耐性 / タルホコムギ / 遺伝資源拡大 / ストレス耐性 / QTL解析 / MSD系統 / コムギ / 組換え近交系統 / 気候変動 / GWAS / アフリカ / マーカー選抜育種 / MSD集団 / 合成コムギ / スーダン / 高温耐性育種 / 異種遺伝資源 / 同質遺伝子系統

Outline of Final Research Achievements

The objective of this study was to elucidate the genetic behavior and the molecular mechanism of high temperature tolerance lines selected from a group of diversity lines introduced into practical wheat varieties from the diversity of a related wild species, Aegilops tauschii. For this purpose, backcrossing recombinant inbred lines (BILs) between high temperature tolerant lines selected in the high-temperature stressed field in Sudan and normal lines were developed and the agronomic traits related to high temperature tolerance and seed traits were analyzed. Also, molecular markers of high temperature response were identified.

Academic Significance and Societal Importance of the Research Achievements

増加する世界人口に対して必要とされる食糧を生産することは、人類社会の発展と安定のための大きい課題である。コムギはイネとともに世界でもっとも重要な穀物であるが、高温に弱く、今後の地球温暖化の環境下で収量が減少すると予想される。本研究では近縁野生種の多様性を実用パンコムギ品種に導入した系統群から選抜された高温耐性系統の量的遺伝子座を解明し、マーカー選抜育種法によって確実に選抜できる方法を開発した。

Research Products

• [Int'l Joint Research] Agricultural Research Corporation(スーダン)
• [Int'l Joint Research] Agricultural Research Corporation(スーダン)
• [Journal Article] Exploitation of tolerance of wheat kernel weight and shape-related traits from Aegilops tauschii under heat and combined heat-drought stresses (2021)
  • Author(s): Idris EGM, Mohamed KN, Gorafi YSA, Yamasaki Y, Takata K, Tahir ISA, Itam MO, Tanaka H, Tsujimoto H
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Issue: 4 Pages: 1830-1830
  • DOI: 10.3390/ijms22041830
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Aegilops tauschii Introgressions Improve Physio-Biochemical Traits and Metabolite Plasticity in Bread Wheat under Drought Stress. (2020)
  • Author(s): Itam M, Abdelrahman M, Yamasaki Y, Mega R, Gorafi Y, Akashi K, Tsujimoto H
  • Journal Title: Agronomy Volume: 10 Issue: 10 Pages: 1588-1588
  • DOI: 10.3390/agronomy10101588
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A population of wheat multiple synthetic derivatives: an effective platform to explore, harness and utilize genetic diversity of Aegilops tauschii for wheat improvement (2018)
  • Author(s): Gorafi Yasir Serag Alnor, Kim June-Sik, Elbashir Awad Ahmed Elawad, Tsujimoto Hisashi
  • Journal Title: Theoretical and Applied Genetics Volume: 131 Issue: 8 Pages: 1615-1626
  • DOI: 10.1007/s00122-018-3102-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] QTL hotspots for combined heat and drought stress resilience in bread wheat grown in Sudanese field (2021)
  • Author(s): Itam M, Gorafi Y, Tahir I, Tsujimoto H
  • Organizer: 第139回日本育種学会講演会
• [Presentation] Harnessing the genetic diversity of wild emmer wheat for genetic improvement of durum wheat (2020)
  • Author(s): Abdalla MYB, Gorafi YSA, Kamal NM, Tahir I, Tsujimoto H
  • Organizer: 第12回中国地域育種学談話会
• [Presentation] Physio-agronomic and metabolite profiling reveal the role of Aegilops tauschii introgressions in wheat lines under drought stress (2020)
  • Author(s): Itam M, Gorafi Y, Izzat T, Tsujimoto H
  • Organizer: 第15回ムギ類研究会
• [Presentation] 生育時の高温処理がパンコムギの高温発芽能力と脂肪酸組成に及ぼす影響 (2020)
  • Author(s): 松永幸子、アリザメグミ、山崎裕司、明石欣也、辻本壽
  • Organizer: 中国地域育種談話会
• [Presentation] チップ栽培を利用したコムギのスピードブリーディングについて (2020)
  • Author(s): 内田孝三、辻本壽
  • Organizer: 第12回中国地域育種学談話会
• [Presentation] パンコムギの高温発芽能力に及ぼす遺伝子および環境の影響 (2020)
  • Author(s): 塚田美彩子、辻本壽
  • Organizer: 第12回中国地域育種学談話会
• [Presentation] Can we find abiotic stress tolerance in wheat related-wild species? - Experiences and lessons learned in pre-breeding of wheat with drought and heat stress tolerance. (2019)
  • Author(s): Tsujimoto, H.
  • Organizer: The 1st International Expert Workshop on Pre-breeding Utilizing Crop Wild Relatives
  • Int'l Joint Research / Invited
• [Presentation] Wheat improvement for heat prone agro-ecologies of Sudan and Sub-Saharan Africa: Breeding and Pre-breeding approaches for climate change resilient varieties. (2019)
  • Author(s): Tahir, I.S.A., Meheesi, S. E. M, Mohammed, I. E. S, Gorafi, Y. S. A., Tsujimoto, H., Tadesse, W., Bassi, F. M. B., and Amiri, A.
  • Organizer: The 1st International Expert Workshop on Pre-breeding Utilizing Crop Wild Relatives
  • Int'l Joint Research / Invited
• [Presentation] Extensive wheat germplasm enhancement to secure food under climate change (2019)
  • Author(s): Tsujimoto, H., Gorafi, Y. S. A., Tahir, I. S. A.
  • Organizer: International Conference of Plant Chromosome Engineering and Functional Genomics for Breeding 2019
  • Int'l Joint Research / Invited
• [Presentation] Innovative wheat breeding technology using novel genetic resources of related wild species (2019)
  • Author(s): Tsujimoto, H.
  • Organizer: Multi-stakeholder Forum: Turning Agricultural and Environmental Challenges into Opportunities for Investment and Innovation in the Aral Sea Basin
  • Int'l Joint Research / Invited
• [Presentation] Mining wild genes/traits for wheat improvement in sustainable agriculture under climate change (2019)
  • Author(s): Tsujimoto, H.
  • Organizer: Cereal Endophyte Workshop
  • Int'l Joint Research / Invited