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Analysis of ethylene biosynthesis regulated by ACC synthase phosphorylation

Research Project

Project/Area Number 18H02196
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39030:Horticultural science-related
Research InstitutionNagoya University

Principal Investigator

Mori Hiroshi  名古屋大学, 生命農学研究科, 教授 (20220014)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
KeywordsACC合成酵素 / タンパク質リン酸化 / タンパク質脱リン酸化 / エチレン生合成 / エチレン / リン酸化 / protein phosphatase / 脱リン酸化 / 質量分析 / 質量分析計
Outline of Final Research Achievements

ACC synthase, ACS, which is rate limiting key enzyme of plant hormone ethylene biosynthesis is phosphorylated by protein kinase CDPK and MAPK respectively. Phosphorylated ACS is stable and dephosphorylated one is unstable in cell. Protein phosphatase, PP2A, consists of subunit A, B, C and B subunit recognizes substrate of specific phosphorylated protein. I tried to identify B” type subunit of PP2A type protein phosphatase in tomato as candidate that recognizes ACS as target. Some candidates of 16 kinds B subunits are selected, but cannot identify B” type subunit finally.

Academic Significance and Societal Importance of the Research Achievements

リン酸化・脱リン酸化によって酵素活性が活性化・不活性化される機構は、よく知られているが、ACC合成酵素のリン酸化は酵素活性に影響せず、酵素の分解に関与し細胞内の酵素量を制御するという新たな分子機構である。本申請課題で扱ったACC合成酵素のリン酸化を介した制御機構は、これまでに示されたプロテアソーム系とは、標的タンパク質を認識する機構が異なる新しいタンパク質分解系の制御機構である。このような新しい機構が明らかにされることの学術的な意義は高く、生命科学の発展に大きく貢献できると期待できる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (15 results)

All 2021 2020 2019 2018

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (14 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] A Ycf2-FtsHi heteromeric AAA-ATPase complex is required for chloroplast protein import2018

    • Author(s)
      Kikuchi Shingo、Asakura Yukari、Imai Midori、Nakahira Yoichi、Kotani Yoshiko、Hashiguchi Yasuyuki、Nakai Yumi、Takafuji Kazuaki、B?dard Jocelyn、Hirabayashi-Ishioka Yoshino、Mori Hitoshi、Shiina Takashi、Nakai Masato
    • Journal Title

      The Plant Cell

      Volume: 30 Issue: 11 Pages: 2677-2703

    • DOI

      10.1105/tpc.18.00357

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 質量分析計を用いたmultiple reaction monitoring (MRM)法による各種植物タンパク質の定量法2021

    • Author(s)
      森 仁志,香村 祐太朗,向井 遥之
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Biochemical Analysis of Stemness-Related Proteins in Arabidopsis2021

    • Author(s)
      槻木 竜二,森 仁志
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 質量分析計を用いたmultiple reaction monitoring (MRM)法によるアサガオFTタンパク質の定量法2021

    • Author(s)
      森 仁志,香村 祐太朗,向井 遥之
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 質量分析計MRM法を用いた各種植物タンパク質の定量法2020

    • Author(s)
      森 仁志,香村 祐太朗,向井 遥之
    • Organizer
      植物化学調節学会第55回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] エンドウの頂芽優勢に関するタンパク質量の検出法2020

    • Author(s)
      森仁志
    • Organizer
      園芸学会 令和2年度春季大会(開催中止)
    • Related Report
      2019 Annual Research Report
  • [Presentation] エンドウの頂芽優勢に関するタンパク質量の検出法2020

    • Author(s)
      森仁志
    • Organizer
      第61回日本植物生理学会年会(開催中止)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 質量分析計を用いたタンパク質量の検出法2019

    • Author(s)
      森仁志
    • Organizer
      植物化学調節学会第54回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 質量分析計を用いたタンパク質量の検出法2019

    • Author(s)
      森仁志
    • Organizer
      第37回日本植物細胞分子生物学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Identification and quantitative measurement of proteins of biosynthesis and signaling plant hormones concerning apical dominance using MRM assays by mass spectrometry2018

    • Author(s)
      H. MORI
    • Organizer
      Auxins and Cytokinins in Plant Development International Symposium 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification and quantitative measurement of proteins of ethylene biosynthesis using MRM assays by mass spectrometry2018

    • Author(s)
      H. MORI
    • Organizer
      The XI International Symposium on the Plant Hormone Ethylene
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification and quantitative measurement of proteins of biosynthesis and signaling plant hormones concerning apical dominance using MRM assays by mass spectrometry2018

    • Author(s)
      H. MORI
    • Organizer
      6th Plant Dormancy Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 質量分析計によるエチレン生合成に関するタンパク質の定量法2018

    • Author(s)
      森仁志
    • Organizer
      植物化学調節学会 第53回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] トマト果実のエチレン生合成・シグナル伝達に関するタンパク質量の検出法2018

    • Author(s)
      森仁志
    • Organizer
      園芸学会 平成30年度秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ラナンキュラス塊根の吸水発芽時に発現するα-galactosidaseタンパク質の発現解析2018

    • Author(s)
      森 仁志、村上恭子
    • Organizer
      園芸学会 平成30年度春季大会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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