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Elucidation of molecular mechanisms of host plant-primary parasitic fungi-secondary parasitic fungi interaction at the single-cell level

Research Project

Project/Area Number 18H02205
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionKobe University

Principal Investigator

Yoshida Kentaro  神戸大学, 農学研究科, 准教授 (40570750)

Co-Investigator(Kenkyū-buntansha) 八丈野 孝  愛媛大学, 農学研究科, 准教授 (10404063)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords1細胞 / RNAシークエンス / マイクロインジェクション / うどんこ病 / オオムギ / コムギ / うどんこ病菌 / エフェクター / 病害抵抗性 / RNA sequencing / レーザーインジェクション / 1細胞
Outline of Final Research Achievements

It is known that avirulence powdery mildew pathogens can infect plant cells that have already been infected with virulence powdery mildew pathogens, while virulence powdery mildew pathogens cannot infect host plant cells where avirulence powdery mildew pathogens have failed to infect. In this study, in order to elucidate the molecular mechanism behind these phenomena, we developed the method to comprehensively analyze the gene expression in a single cell infected with powdery mildew pathogens and the injection method to introduce proteins derived from the host plants and the powdery mildew pathogens into a single host plant cell.

Academic Significance and Societal Importance of the Research Achievements

植物が病気になるのは、糸状菌などの植物病原菌による。植物病原菌と植物の感染を巡る攻防は細胞レベルで起きている。これまでにうどんこ病菌とムギ類植物の1細胞レベルで詳細な観察がされてきたが、その分子の実体の全容は解明されていない。うどんこ病菌とムギ植物の攻防を巡る両者の分子の動態と機能を明らかにするための新たな研究技術を開発したことで、これらの分子を解明への貢献が期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (11 results)

All 2021 2020 2019 2018

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Nicotinamide Mononucleotide Potentiates Resistance to Biotrophic Invasion of Fungal Pathogens in Barley2021

    • Author(s)
      Ueda Kana、Nakajima Yuichi、Inoue Hiroshi、Kobayashi Kappei、Nishiuchi Takumi、Kimura Makoto、Yaeno Takashi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 5 Pages: 2696-2696

    • DOI

      10.3390/ijms22052696

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fast and Inexpensive Phenotyping and Genotyping Methods for Evaluation of Barley Mutant Population2020

    • Author(s)
      Kawamoto Yudai、Toda Hirotaka、Inoue Hiroshi、Kobayashi Kappei、Yamaoka Naoto、Araki Takuya、Yaeno Takashi
    • Journal Title

      Plants

      Volume: 9 Issue: 9 Pages: 1153-1153

    • DOI

      10.3390/plants9091153

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] High Humidity Causes Abnormalities in the Process of Appressorial Formation of Blumeria graminis f. sp. hordei.2020

    • Author(s)
      Koreyuki Sugai, Hiroshi Inoue, Chie Inoue, Mayuko Sato, Mayumi Wakazaki, Kappei Kobayashi, Masamichi Nishiguchi, Kiminori Toyooka, Naoto Yamaoka, Takashi Yaeno
    • Journal Title

      Pathogens

      Volume: 9 Issue: 1 Pages: 45-45

    • DOI

      10.3390/pathogens9010045

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Formvar membrane laid on artificial medium induces haustorium-like structure formation in powdery mildew fungi2019

    • Author(s)
      Naoto Yamaoka, Eiji Tanaka, Tsubasa Ogasahara, Honoka Tani, Kappei Kobayashi, Takashi Yaeno
    • Journal Title

      Mycoscience

      Volume: 60 Issue: 5 Pages: 298-301

    • DOI

      10.1016/j.myc.2019.06.006

    • NAID

      40022012811

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] レーザーマイクロインジェクション技術を用いたオオムギうどんこ病菌エフェクタータンパク質の単一細胞導入法の開発2020

    • Author(s)
      小川翔也・清水茜・吉田健太郎・小林活平・八丈野孝
    • Organizer
      令和2年度日本植物病理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] オオムギうどんこ病菌エフェクタータンパク質を単一細胞へ導入するためのマイクロインジェクション技術の開発2019

    • Author(s)
      小川翔也・清水茜・吉田健太郎・小林活平・八丈野孝
    • Organizer
      第14回ムギ類研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ムギ類うどんこ病菌の周期的な感染機構と宿主の防御応答2019

    • Author(s)
      川見祐樹・溝尾直子・宅見薫雄・吉田健太郎
    • Organizer
      第14回ムギ類研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Gene expression dynamics of the obligate filamentous pathogen Blumeria graminis and its host plant under the field environments2019

    • Author(s)
      Kentaro Yoshida, Naoko Mizoo, Kazumasa Wakamori, Kaori Oikawa, Hiroki Yaegashi, Satoshi Natsume, Aiko Uemura, Akira Abe, Ryohei Terauchi, Hiroshi Mineno, Shigeo Takumi
    • Organizer
      IS-MPMI XVIII Congress
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ムギ類うどんこ病菌感染細胞におけるsingle-cell RNA-seqの開発2019

    • Author(s)
      三野航司朗・久保稔・八丈野孝・宅見薫雄・吉田健太郎
    • Organizer
      平成31年度 日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] レーザーインジェクション技術によるsingle-cellエフェクター発現法の開発2019

    • Author(s)
      清水茜・吉田健太郎・小林括平・山岡直人・八丈野孝
    • Organizer
      平成31年度 日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ムギ類single-cell RNA-seq技術の開発2018

    • Author(s)
      三野航司朗・久保稔・八丈野孝・宅見薫雄・吉田健太郎
    • Organizer
      第13回 ムギ類研究会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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