Virulence mechanism of rice sheath blight -Identification of effectors-

• Project/Area Number: 18H02206
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: Okayama University
• Principal Investigator: Noutoshi Yoshiteru 岡山大学, 環境生命科学研究科, 准教授 (70332278)
• Co-Investigator(Kenkyū-buntansha): 津下 誠治 京都府立大学, 生命環境科学研究科, 教授 (10254319)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2020: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2019: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: 紋枯病菌 / Rhizoctonia solani / エフェクター / 感染生理 / ミナトカモジグサ / イネ / 活物寄生

Outline of Final Research Achievements

The filamentous fungus Rhizoctonia solani causes sheath blight, an important disease of rice. Its infection strategy remains unclear. In this study, we analyzed the expression of the small secretory protein (so-called effector) encoding genes of Rhizoctonia solani during the infection process in a model pathosystem developed using Brachypodium distachyon, an experimental monocotyledonous plant. As a result, we confirmed that the fungus uses them in the early infection stage before necrosis induction. In contrast to conventional recognition, it was suggested that the fungus undergoes a biotrophic stage of suppressing plant immunity before killing the host.

Academic Significance and Societal Importance of the Research Achievements

本菌は激しい壊死斑を誘導するため、宿主を殺して栄養を摂取する殺生菌に分類されています。本研究により、本菌は宿主を殺す前に組織内に侵入し、そこで分泌型のタンパク質であるエフェクターを放出することで、宿主免疫を抑制しながら寄生を成立させる活物寄生と呼ばれるステップを経ると推測されました。植物は活物寄生菌に対する効率的な免疫系を持つことから、その原理を利用した本菌への耐病性付与の有効性が示唆されました。

Research Products

• [Journal Article] Identification of effector candidate genes of Rhizoctonia solani AG-1 IA expressed during infection in Brachypodium distachyon (2020)
  • Author(s): Abdelsalam, S.H.S., Kouzai, Y., Watanabe, M., Inoue, K., Matsui, H., Yamamoto, M., Ichinose, Y., Toyoda, K., Tsuge, S., Mochida, K., Noutoshi, Y.
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 14889-14889
  • DOI: 10.1038/s41598-020-71968-x
  • NAID: 120006891212
  • Peer Reviewed / Open Access
• [Journal Article] BdWRKY38 is required for the incompatible interaction of Brachypodium distachyon with the necrotrophic fungus Rhizoctonia solani (2020)
  • Author(s): 10)Kouzai, Y., Shimizu, M., Inoue, K., Uehara-Yamaguchi, Y., Takahagi, K., Nakayama, R., Matsuura, T., Mori, I., Hirayama, T., Abdelsalam, S.H.S., Noutoshi, Y., Mochida, K.
  • Journal Title: The Plant Journal Volume: 104 Issue: 4 Pages: 995-995
  • DOI: 10.1111/tpj.14976
  • NAID: 120006953495
  • Peer Reviewed / Open Access
• [Journal Article] 植物の病害抵抗性を高める物質 (2019)
  • Author(s): 能年義輝
  • Journal Title: アグリバイオ Volume: 3 Pages: 30-34
• [Journal Article] Benzothiadiazole, a plant defense inducer, negatively regulates sheath blight resistance in Brachypodium distachyon (2018)
  • Author(s): Kouzai Yusuke、Noutoshi Yoshiteru、Inoue Komaki、Shimizu Minami、Onda Yoshihiko、Mochida Keiichi
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 17358-17358
  • DOI: 10.1038/s41598-018-35790-w
  • Peer Reviewed / Open Access
• [Presentation] 紋枯病菌がミナトカモジグサ感染時に発現するエフェクター候補遺伝子の探索同定 (2020)
  • Author(s): Sobhy S.H. Abdelsalam, 香西雄介, 渡邉恵, 井上小牧, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 津下誠治, 持田恵一, 能年義輝
  • Organizer: 令和2年度日本植物病理学会関西部会
• [Presentation] Characterization of expression profiles of effector genes of Rhizoctonia solani on Brachypodium distachyon (2020)
  • Author(s): Abdelsalam, SSH., Isoya, S., Watanabe, M., Kouzai. Y., Matsui, M., Yamamoto, M., Ichinose, Y., Toyoda, K., Mochida, K., Tsuge, S., Noutoshi, Y.
  • Organizer: 令和2年度日本植物病理学会大会
• [Presentation] An approach to study rice sheath blight disease using Brachypodium distachyon (2019)
  • Author(s): Yoshiteru Noutoshi
  • Organizer: 2019 IS-MPMI XVIII Congress satellite meeting
  • Int'l Joint Research / Invited
• [Presentation] Time-series transcriptome analysis of Brachypodium distachyon in response to the infection by Rhizoctonia solani (2019)
  • Author(s): Kouzai, Y., Inoue, K, Shimizu, M., Uehara, Y., Takahagi, K., Noutoshi, Y., Mochida, K.
  • Organizer: 2019 IS-MPMI XVIII Congress
  • Int'l Joint Research
• [Presentation] Characterization of expression profiles of effector genes of Rhizoctonia solani on Brachypodium distachyon (2019)
  • Author(s): Abdelsalam, SSH., Isoya, S., Watanabe, M., Kouzai. Y., Matsui, M., Yamamoto, M., Ichinose, Y., Toyoda, K., Tsuge, S., Noutoshi, Y.
  • Organizer: 2019 IS-MPMI XVIII Congress
  • Int'l Joint Research
• [Presentation] Rapid activation of WRKY-dependent immunity facilitates native resistance against the sheath blight pathogen, Rhizoctonia solani, in Brachypodium distachyon (2019)
  • Author(s): Kouzai, Y., Inoue, K, Shimizu, M., Uehara-Yamaguchi, Y., Takahagi, K., Noutoshi, Y., Mochida, K
  • Organizer: The 4th international Brachypodium conference
  • Int'l Joint Research
• [Presentation] イネ科モデル植物ミナトカモジグサの微生物分子パターン応答性の解析 (2019)
  • Author(s): 小笠原翼, 香西雄介, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 持田恵一, 能年義輝
  • Organizer: 令和元年度日本植物病理学会関西部会
• [Presentation] Rhizoctonia solaniの感染初期過程に発現するエフェクター様遺伝子の同定 (2019)
  • Author(s): Sobhy S.H. Abdelsalam, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 香西雄介, 持田恵一, 津下誠治, 能年義輝
  • Organizer: 令和元年度日本植物感染生理談話会
• [Presentation] モデル単子葉植物ミナトカモジグサの微生物分子パターン応答性の解析 (2019)
  • Author(s): 小笠原翼, 香西雄介, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 持田恵一, 能年義輝
  • Organizer: 令和元年度日本植物感染生理談話会
• [Presentation] 紋枯病菌感染後におけるミナトカモジグサの時系列比較トランスクリプトーム解析 (2019)
  • Author(s): 香西雄介・井上小慎・清水みなみ、高萩航太郞、能年義輝、持田恵一
  • Organizer: H31年度日本植物病理学会大会
• [Presentation] 微生物分子パターンに対するミナトカモジグサの活性酸素種生成特性の解析 (2018)
  • Author(s): 小笠原翼・松井英譲・山本幹博・一瀬勇規・豊田和弘・香西雄介・能年義輝
  • Organizer: 第53回植物感染生理談話会
• [Presentation] 微生物分子パターンに対するミナトカモジグサの活性酸素種生成特性の解析 (2018)
  • Author(s): 小笠原翼・松井英譲・山本幹博・一瀬勇規・豊田和弘・香西雄介・能年義輝
  • Organizer: H30年度日本植物病理学会関西部会
• [Presentation] Production of reactive oxygen species in Brachypodium distachyon to various microbe-associated molecular patterns (2018)
  • Author(s): Tsubasa Ogasahara, Hidenori Matsui, Mikihiro Yamamoto, Yuki Ichinose, Kazuhiro Toyoda, Yusuke Kouzai, Yoshiteru Noutoshi
  • Organizer: 第1回オオムギ資源開発研究セミナー
• [Presentation] Characterization of expression profiles of effector genes of Rhizoctonia solani on Brachypodium distachyon (2018)
  • Author(s): Sobhy SH Abdelsalam, Hidenori Matsui, Mikihiro Yamamoto, Yuki Ichinose, Kazuhiro Toyoda, Yusuke Kouzai, Seiji Tsuge, Yoshiteru Noutoshi,
  • Organizer: 第1回オオムギ資源開発研究セミナー
• [Presentation] 紋枯病菌のエフェクター候補タンパク質のミナトカモジグサにおける病原性を評価するための白葉枯病菌を用いた異種発現系の構築 (2018)
  • Author(s): 能年義輝, 磯谷怜, 渡邉恵, 香西雄介, Sobhy SH Aldelsalam, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 津下誠治
  • Organizer: 第1回オオムギ資源開発研究セミナー