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Transmission mechanisms of a novel clade of bacterial endosymbionts of the coconut beetle that manipulate host reproduction

Research Project

Project/Area Number 18H02207
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionKyushu University

Principal Investigator

Takano Shun-ichiro  九州大学, 農学研究院, 助教 (90725045)

Co-Investigator(Kenkyū-buntansha) 古屋 成人  九州大学, 農学研究院, 教授 (10211533)
津田 みどり  九州大学, 農学研究院, 准教授 (20294910)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Keywords共生微生物 / 生物的防除 / 侵入害虫 / 細胞質不和合 / 共生細菌 / 胞質不和合 / 侵入種
Outline of Final Research Achievements

In this study, we have proposed a new genus and species, “Candidatus Mesenet longicola”, for the bacterial symbionts, which induce cytoplasmic incompatibility (CI) in the coconut beetle, based on the draft genome and morphological properties of the bacteria. Field studies showed that there were beetles infected with both Ca. M. longicola and Wolbachia in Vietnam. Cross tests indicate that doubly infected insects have a reproductive advantage relative to that of single infected insects and uninfected insects. These findings are considered important to develop a pest control strategy using Ca. M. longicola.

Academic Significance and Societal Importance of the Research Achievements

昆虫の細胞内に共生し、寄主の母から子に伝播する細菌は非感染雌が感染雄と交尾した時、その雌の卵を殺すという能力がある。これは細胞質不和合性(CI)と呼ばれており、感染雄を不妊雄とできるため、害虫防除への応用が期待されているが、これまでの研究はWolbachiaに限定されていた。今回、我々がCIを引き起こす新たな細菌のドラフトゲノムを得て、分類学的地位を明らかにし、新属新種として命名提案したことは、CI研究の発展に寄与すると考えられる。また、野外、室内実験により、重複感染がCIに影響を及ぼす可能性を示唆したことは、これら細菌を利用する害虫防除法の確立のため重要と考えられる。

Report

(4 results)
  • 2022 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (6 results)

All 2021 2020 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results) (of which Invited: 1 results)

  • [Int'l Joint Research] Nong Lam University(ベトナム)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] 南西諸島におけるキムネクロナガハムシの分布2021

    • Author(s)
      高野俊一郎
    • Journal Title

      昆虫と自然

      Volume: 56(12) Pages: 7-11

    • Related Report
      2020 Annual Research Report
  • [Journal Article] “Candidatus Mesenet longicola”: Novel Endosymbionts of Brontispa longissima that Induce Cytoplasmic Incompatibility2021

    • Author(s)
      Takano Shun-ichiro、Gotoh Yasuhiro、Hayashi Tetsuya
    • Journal Title

      Microbial Ecology

      Volume: - Issue: 2 Pages: 512-522

    • DOI

      10.1007/s00248-021-01686-y

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] “Candidatus Mesenet longicola”キムネクロナガハムシに感染し細胞質不和合(CI)を引き起こす新規細菌2021

    • Author(s)
      高野俊一郎
    • Organizer
      第65回日本応用動物昆虫学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 外来生物の侵入メカニズムと生物的防除法2021

    • Author(s)
      高野俊一郎
    • Organizer
      第379回昆虫学土曜セミナー
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ベトナムにおける侵入害虫キムネクロナガハムシに感染する新規ボルバキア様細菌の分布とCI能力2020

    • Author(s)
      高野俊一郎
    • Organizer
      日本応用動物昆虫学会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2024-01-30  

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