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Studies on signal transduction pathway of regulation of secondary metabolism in plant-protective bacteria

Research Project

Project/Area Number 18H02209
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

Takeuchi Kasumi  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (40370663)

Co-Investigator(Kenkyū-buntansha) 諸星 知広  宇都宮大学, 工学部, 准教授 (90361360)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2020: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords植物保護細菌 / 二次代謝制御 / シグナル物質 / 抗菌性物質 / 調節型small RNA / 比較トランスクリプトーム解析
Outline of Final Research Achievements

Pseudomonas protegens and other root-colonizing fluorescent pseudomonads are effective strains that suppress plant diseases by producing several extracellular enzymes and secondary metabolites with antibiotic activities. Based on the findings of RNA-seq gene expression data analysis of P. protegens, we identified and characterized an effective molecule that regulates the expression of genes supposed to be involved in secondary metabolism. This molecule increases the biocontrol efficacy of P. protegens for the suppression of soil-borne pathogen caused by oomycete Pythium ultimum. These results suggest an important role of this molecule in the niche adaptation and disease suppressive activity of P. protegens in the rhizosphere.

Academic Significance and Societal Importance of the Research Achievements

植物保護細菌が周辺環境の変化に応じて遺伝子発現の調節を行っていることをRNA Seq等により解析したことにより、網羅的な発現変動パターンの精査なデータの取得に成功した。これは植物保護能力との機能の相関を示す重要な成果であるとともに、遺伝子発現制御の新規メカニズムを示したものである。シグナル認識に関わる因子については推定遺伝子を同定し引き続き解析を行っている。また本課題で同定した亢進シグナルについて、植物保護細菌とともに土壌に処理したところ、農業上問題となっている卵菌類による土壌病害に対し植物保護細菌の効果を高めることが明らかになったことから、農業利用に資する成果となった。

Report

(5 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Products Report
  • Research Products

    (17 results)

All 2023 2022 2021 2020 2019 2018 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 1 results)

  • [Journal Article] Comparative Genome Analysis Reveals Differences in Biocontrol Efficacy According to Each Individual Isolate Belonging to Rhizospheric Fluorescent Pseudomonads2021

    • Author(s)
      Sakuraoka Ryohei、Someya Nobutaka、Takeuchi Kasumi、Suzuki Tomohiro、Morohoshi Tomohiro
    • Journal Title

      Microbes and Environments

      Volume: 36 Issue: 3 Pages: n/a

    • DOI

      10.1264/jsme2.ME21034

    • NAID

      130008068651

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Discovery of an Antibiotic-Related Small Protein of Biocontrol Strain Pseudomonas sp. Os17 by a Genome-Mining Strategy2020

    • Author(s)
      Takeuchi Kasumi、Tsuchiya Wataru、Fujimoto Zui、Yamada Kosumi、Someya Nobutaka、Yamazaki Toshimasa
    • Journal Title

      Frontiers in Microbiology

      Volume: 11

    • DOI

      10.3389/fmicb.2020.605705

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Diversity of Antibiotic Biosynthesis Gene-possessing Rhizospheric Fluorescent Pseudomonads in Japan and Their Biocontrol Efficacy2020

    • Author(s)
      Someya Nobutaka、Kubota Masaharu、Takeuchi Kasumi、Unno Yusuke、Sakuraoka Ryohei、Morohoshi Tomohiro
    • Journal Title

      Microbes and Environments

      Volume: 35 Issue: 2 Pages: n/a

    • DOI

      10.1264/jsme2.ME19155

    • NAID

      130007829092

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An overview of recent genomic research on biocontrol Pseudomonad strains isolated from the field in Japan2019

    • Author(s)
      Kasumi Takeuchi, Nobutaka Someya
    • Journal Title

      Japan Agricultural Research Quarterly

      Volume: 53 Pages: 87-91

    • NAID

      130007636825

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 植物保護細菌によるダイズ茎疫病の防除効果に対するグルタミン酸の影響2022

    • Author(s)
      竹内香純、姜昌杰、小木曽真佐代、瀬尾茂美
    • Organizer
      令和4年度 日本植物病理学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 植物生育促進効果を有するPseudomonas sp. Sm006のゲノム解析2021

    • Author(s)
      竹内香純、諸星知広、染谷信孝
    • Organizer
      令和3年度 日本植物病理学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Pseudomonas protegensの抗菌性変異株の比較トランスクリプトーム解析2020

    • Author(s)
      竹内香純、染谷信孝、諸星知広
    • Organizer
      平成30年度 日本植物病理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物保護細菌における二次代謝産物産生能間クロストーク2019

    • Author(s)
      染谷信孝、竹内香純、諸星知広
    • Organizer
      日本微生物生態学会 第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物保護細菌の調節型small RNAの発現と病害防除効果に対するアミノ酸の影響2019

    • Author(s)
      竹内香純、瀬尾茂美
    • Organizer
      平成30年度 日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Identification of antibiotic-related genes of biocontrol strain Pseudomonas sp. Os17 by genome mining strategies2018

    • Author(s)
      Kasumi Takeuchi, Wataru Tsuchiya, Nobutaka Someya, Toshimasa Yamazaki
    • Organizer
      第11回 国際PGPR研究会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細菌の生存戦略から学んだこと2018

    • Author(s)
      竹内香純
    • Organizer
      アカデミアで活躍されている女性研究者から在学生への助言~岡山大学農学部同窓会セミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Book] 植物保護能力を有する蛍光性Pseudomonasの機能と生態 : 植物保護細菌の多彩な武器2019

    • Author(s)
      染谷信孝、竹内香純、諸星知広
    • Total Pages
      8
    • Publisher
      化学と生物 : 日本農芸化学会会誌
    • Related Report
      2019 Annual Research Report
  • [Remarks] 農研機構 生物機能利用研究部門 植物・微生物機能利用研究領域 植物微生物機能ユニット

    • URL

      http://www.naro.affrc.go.jp/laboratory/nias/introduction/chart/0403/index.html

    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] ダイズの土壌伝染性病害防除方法2023

    • Inventor(s)
      竹内 香純, 姜 昌杰, 瀬尾 茂美
    • Industrial Property Rights Holder
      国立研究開発法人農業・食品産業技術総合研究機構
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2023
    • Related Report
      Products Report
  • [Patent(Industrial Property Rights)] 植物成長促進又は植物保護の能力を有する微生物2020

    • Inventor(s)
      竹内香純、染谷信孝
    • Industrial Property Rights Holder
      竹内香純、染谷信孝
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-206870
    • Filing Date
      2020
    • Related Report
      2020 Annual Research Report
  • [Patent(Industrial Property Rights)] 植物の土壌伝染性病害防除方法2020

    • Inventor(s)
      竹内香純、瀬尾茂美
    • Industrial Property Rights Holder
      竹内香純、瀬尾茂美
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2020 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] ダイズの土壌伝染性病害防除方法2020

    • Inventor(s)
      竹内香純、姜 昌杰、瀬尾茂美
    • Industrial Property Rights Holder
      竹内香純、姜 昌杰、瀬尾茂美
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2019 Annual Research Report

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Published: 2018-04-23   Modified: 2024-03-28  

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