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Elucidation of insect steroid hormone biosynthetic pathway

Research Project

Project/Area Number 18H02214
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39050:Insect science-related
Research InstitutionKyoto University

Principal Investigator

Ono Hajime  京都大学, 農学研究科, 准教授 (70452282)

Co-Investigator(Kenkyū-buntansha) 片岡 宏誌  東京大学, 大学院新領域創成科学研究科, 教授 (60202008)
Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2020: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2018: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywordsエクダイソン / ステロイドホルモン / 生合成 / 前胸腺 / 重水素ラベル体 / 生理活性物質 / ショウジョウバエ / カイコ / 立体異性体 / 有機合成 / 細胞培養 / 植物トリテルペノイド / 生合成阻害 / 生合成酵素 / S2細胞 / 脱皮・変態 / 7-デヒドロコレステロール
Outline of Final Research Achievements

The biosynthetic pathway of the insect steroid hormone ecdysone has not been elucidated. Notably, the reaction process in which the ecdysteroid-specific skeleton has not been characterized. The pathway has been considered as the rate-limiting step, and therefore, is an important biological reaction process. In this study, two novel compounds, T1-a and T2-c, have been identified to be possible candidate intermediates. We synthesized deuterium-labeled T1-a and T2-c compounds. The synthesized deuterium-labeled compounds were incubated with the prothoracic glands of the silkworm which is the steroid hormone biosynthetic tissue, and we confirmed that the labeled compounds were converted to labeled ecdysone. Thus, compounds T1-a and T2-c were converted to ecdysone in the prothoracic glands, therefore, we conclude that these compounds are intermediates of the unknown pathway.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果により、エクダイソンが関与する昆虫の発育制御や環境応答の機構の解明につながると同時に、農薬開発等の応用研究にも結びつくことが期待できる。また、昆虫のみならず哺乳類のステロイドホルモン生合成の中間体でもある7-デヒドロコレステロールの重水素ラベル体の簡易合成法を確立した。本法を用いることで7-デヒドロコレステロールおよび類縁化合物のラベル体の有機合成が可能となる。ステロイドホルモンは様々な生物の発育を制御しているため、基礎研究における波及性が大きいと考えられる。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (21 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (3 results) Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (10 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Remarks (3 results)

  • [Int'l Joint Research] North Carolina State University(米国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Southwest University(中国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Universiti Putra Malaysia(マレーシア)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Oviposition stimulants underlying different preferences between host races in the leaf-mining moth Acrocercops transecta (Lepidoptera: Gracillariidae)2022

    • Author(s)
      Katte Tomoko、Shimoda Shota、Kobayashi Takuya、Wada-Katsumata Ayako、Nishida Ritsuo、Ohshima Issei、Ono Hajime
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 14498-14498

    • DOI

      10.1038/s41598-022-18238-0

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Functional characterization of an olfactory receptor in the Oriental fruit fly, Bactrocera dorsalis, that responds to eugenol and isoeugenol2022

    • Author(s)
      Hajime Ono
    • Journal Title

      Comparative Biochemistry and Physiology Part B

      Volume: 258 Pages: 110696-110696

    • DOI

      10.1016/j.cbpb.2021.110696

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The plant-derived triterpenoid, cucurbitacin B, but not cucurbitacin E, inhibits the developmental transition associated with ecdysone biosynthesis in Drosophila melanogaster2021

    • Author(s)
      Miwako Toyofuku, Daiki Fujinaga, Kazue Inaba, Tomoki Funahashi, Yuuta Fujikawa, Hideshi Inoue, Hiroshi Kataoka, Ryusuke Niwa, Hajime Ono
    • Journal Title

      Journal of Insect Physiology

      Volume: 134 Pages: 104294-104294

    • DOI

      10.1016/j.jinsphys.2021.104294

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recent advancements in studies on chemosensory mechanisms underlying detection of semiochemicals in Dacini fruit flies of economic importance ( Diptera: Tephritidae ),2021

    • Author(s)
      Hajime Ono, Alvin Kah-Wei Hee, Hongbo Jiang
    • Journal Title

      Insects

      Volume: 12 Issue: 2 Pages: 106-106

    • DOI

      10.3390/insects12020106

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Predominant accumulation of a 3-hydroxy-γ-decalactone in the male rectal gland complex of the Japanese orange fly, Bactrocera tsuneonis2020

    • Author(s)
      Hajime Ono, Masataka Nakahira, Satoshi Ohno, Jun Otake, Tomoya Kanno, Isao Tokushima, Yoshimitsu Higashiura, Ichiro Nishi, Ritsuo Nishida
    • Journal Title

      Bioscience, biotechnology, and biochemistry

      Volume: 84 Issue: 1 Pages: 25-30

    • DOI

      10.1080/09168451.2019.1664892

    • NAID

      120006773161

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Chemical substances underlying different host utilization between host races in the leaf-mining moth Acrocercops transecta (Lepidoptera: Gracillariidae)2022

    • Author(s)
      Hajime Ono, Issei Ohshima
    • Organizer
      3rd Joint Meeting of International Society of Chemical Ecology and Asia-Pacific Association of Chemical Ecologists
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物精油を用いたミバエ科昆虫嗅覚受容体のリガンド探索2022

    • Author(s)
      小野 肇
    • Organizer
      第66回日本応用動物昆虫学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] クルミホソガの寄主転換に関わるネジキ有毒成分の探索2021

    • Author(s)
      江原 瑶柄、小林 拓矢、大島 一正、小野 肇
    • Organizer
      第65回日本応用動物昆虫学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ウンシュウミカン被害果からのリアルタイムPCR法によるミカンバエDNAの検出2021

    • Author(s)
      大田 祥平、菅野 伸哉、小野 肇
    • Organizer
      第65回日本応用動物昆虫学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ステロール代謝およびエクダイソン生合成を介した植物トリテルペノイドcucurbitacin 類がショウジョウバエの発育に及ぼす影響2020

    • Author(s)
      小野 肇、豊福 美和子、藤永 大輝、稲葉 和恵、船橋 智輝、藤川 雄太、井上英史、片岡 宏誌、丹羽 隆介
    • Organizer
      日本農薬学会 第45回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Ecdysone 生合成未知経路の解明2020

    • Author(s)
      船橋 智輝,藤永 大輝,齊藤 惇基,片岡 宏誌,小野 肇
    • Organizer
      日本農薬学会 第45回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物トリテルペノイド cucurbitacin B によるエクダイソン生合成の阻害2020

    • Author(s)
      小野 肇、豊福 美和子、藤永 大輝、稲葉 和恵、船橋 智輝、藤川 雄太、井上英史、片岡 宏誌、丹羽 隆介
    • Organizer
      第64回 日本応用動物昆虫学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Functional characterization of olfactory receptors in Dacini fruit flies (Diptera: Tephritidae) that respond to semiochemicals2019

    • Author(s)
      Hajime Ono, Hitomi Miyazaki, Jun Otake, Hidefumi Mitsuno, Katsuhisa Ozaki, Ryohei Kanzaki, Anna Chui-Ting Chieng, Alvin Kah-Wei Hee, Ritsuo Nishida
    • Organizer
      Asia-Pacific Association of Chemical Ecologists
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] エクジステロイド生合成関連酵素Noppera-boの阻害物質の探索2019

    • Author(s)
      豊福美和子,稲葉和恵,藤川雄太,井上英史,丹羽隆介,小野肇
    • Organizer
      日本農薬学会第44回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Ecdysone 生合成未知経路の解明に向けて : 7-dehydrocholesterol 類縁体の脱皮活性評価2019

    • Author(s)
      船橋 智輝,齊藤 惇基,小野 肇
    • Organizer
      第63回日本応用動物昆虫学会大会
    • Related Report
      2018 Annual Research Report
  • [Remarks] 小野 肇 Hajime Ono

    • URL

      https://sites.google.com/view/hajime-ono/home

    • Related Report
      2022 Annual Research Report
  • [Remarks] 化学生態学分野

    • URL

      http://www.chemeco.kais.kyoto-u.ac.jp/

    • Related Report
      2022 Annual Research Report 2018 Annual Research Report
  • [Remarks] 化学生態学分野

    • URL

      https://www.chemeco.kais.kyoto-u.ac.jp/access.html

    • Related Report
      2020 Annual Research Report

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Published: 2018-04-23   Modified: 2024-01-30  

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