| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2020: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2018: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Outline of Final Research Achievements |
The biosynthetic pathway of the insect steroid hormone ecdysone has not been elucidated. Notably, the reaction process in which the ecdysteroid-specific skeleton has not been characterized. The pathway has been considered as the rate-limiting step, and therefore, is an important biological reaction process. In this study, two novel compounds, T1-a and T2-c, have been identified to be possible candidate intermediates. We synthesized deuterium-labeled T1-a and T2-c compounds. The synthesized deuterium-labeled compounds were incubated with the prothoracic glands of the silkworm which is the steroid hormone biosynthetic tissue, and we confirmed that the labeled compounds were converted to labeled ecdysone. Thus, compounds T1-a and T2-c were converted to ecdysone in the prothoracic glands, therefore, we conclude that these compounds are intermediates of the unknown pathway.
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