Development of Information Base on Microbiota of Japanese Forests

• Project/Area Number: 18H02233
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 40010:Forest science-related
• Research Institution: The University of Tokyo
• Principal Investigator: Isobe Kazuo 東京大学, 大学院農学生命科学研究科(農学部), 助教 (30621833)
• Co-Investigator(Kenkyū-buntansha): 大手 信人 京都大学, 情報学研究科, 教授 (10233199)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000); Fiscal Year 2020: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2019: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2018: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
• Keywords: 土壌微生物 / 森林土壌 / 窒素循環 / 生物地理 / 森林

Outline of Final Research Achievements

We investigated the soil microbial community at 40 forest sites in 22 regions across Japan. We aimed to clarify the geographic distribution of species composition and physiological traits of soil microbial communities, and their regulation on nitrogen cycling. We also aimed to develop the information base on soil microbial communities of Japanese forests. The results showed that the geographic distribution of species composition of soil microbial communities can be largely explained by the physiological traits of microbial species, and that the species composition is directly reflected in the rate of nitrogen cycling in forests.

Academic Significance and Societal Importance of the Research Achievements

森林土壌において微生物は物質循環の形成や植物への養分供給などの機能を担い、生態系を支えている。森林において微生物が果たす機能的役割の大きさ、あるいは腸内細菌や病原菌など微生物に対する社会的認知が広がっていることを鑑みても、日本の森林土壌の微生物相に関する基本情報を整備・体系化し、生態学的研究を支える情報基盤を構築する必要がある。本研究の成果は、日本の森林が有する特徴を新たな視点から捉え直す機会を提供すると同時に、微生物相の生態的・社会的影響の評価を可能とするための情報基盤となる。

Research Products

• [Int'l Joint Research] カリフォルニア大学アーバイン校(米国)
• [Int'l Joint Research] カリフォルニア大学アーバイン校(米国)
• [Int'l Joint Research] カリフォルニア大学アーバイン校(米国)
• [Journal Article] Distinct Community Composition of Previously Uncharacterized Denitrifying Bacteria and Fungi across Different Land-Use Types (2020)
  • Author(s): Fujimura Reiko、Azegami Yoichi、Wei Wei、Kakuta Hiroko、Shiratori Yutaka、Ohte Nobuhito、Senoo Keishi、Otsuka Shigeto、Isobe Kazuo
  • Journal Title: Microbes and Environments Volume: 35 Issue: 1 Pages: n/a
  • DOI: 10.1264/jsme2.ME19064
  • NAID: 130007792089
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access
• [Journal Article] Phylogenetic conservation of soil bacterial responses to simulated global changes (2020)
  • Author(s): Isobe Kazuo、Bouskill Nicholas J.、Brodie Eoin L.、Sudderth Erika A.、Martiny Jennifer B. H.
  • Journal Title: Philosophical Transactions of the Royal Society B: Biological Sciences Volume: 375 Issue: 1798 Pages: 1-12
  • DOI: 10.1098/rstb.2019.0242
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Consequences of microbial diversity in forest nitrogen cycling: diverse ammonifiers and specialized ammonia oxidizers (2020)
  • Author(s): Kazuo Isobe, Yuta Ise, Hiroyu Kato, Tomoki Oda, Christian E Vincenot, Keisuke Koba, Ryunosuke Tateno, Keishi Senoo, Nobuhito Ohte
  • Journal Title: The ISME journal Volume: 14 Issue: 1 Pages: 12-25
  • DOI: 10.1038/s41396-019-0500-2
  • NAID: 120006800755
  • Peer Reviewed / Open Access
• [Journal Article] Phylogenetic conservation of bacterial responses to soil nitrogen addition across continents (2019)
  • Author(s): Isobe Kazuo、Allison Steven D.、Khalili Banafshe、Martiny Adam C.、Martiny Jennifer B. H.
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 1-8
  • DOI: 10.1038/s41467-019-10390-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] High soil microbial activity in the winter season enhances nitrogen cycling in a cool-temperate deciduous forest (2018)
  • Author(s): Isobe Kazuo、Oka Hiroaki、Watanabe Tsunehiro、Tateno Ryunosuke、Urakawa Rieko、Liang Chao、Senoo Keishi、Shibata Hideaki
  • Journal Title: Soil Biology and Biochemistry Volume: 124 Pages: 90-100
  • DOI: 10.1016/j.soilbio.2018.05.028
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Highly abundant acidophilic ammonia-oxidizing archaea causes high rates of nitrification and nitrate leaching in nitrogen-saturated forest soils (2018)
  • Author(s): Isobe Kazuo、Ikutani Junko、Fang Yunting、Yoh Muneoki、Mo Jiangming、Suwa Yuichi、Yoshida Makoto、Senoo Keishi、Otsuka Shigeto、Koba Keisuke
  • Journal Title: Soil Biology and Biochemistry Volume: 122 Pages: 220-227
  • DOI: 10.1016/j.soilbio.2018.04.021
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 日本の森林における土壌微生物群集の物質代謝機能 -メタゲノムと生物地理- (2019)
  • Author(s): 伊澤航太郎、伊勢裕太、髙見英人、大手信人、妹尾啓史、磯部一夫
  • Organizer: 日本微生物生態学会
• [Presentation] 土壌のバクテリア群集の窒素応答は進化系統的に保存されている (2019)
  • Author(s): 磯部一夫
  • Organizer: 日本微生物生態学会
• [Presentation] 土壌微生物群集の環境応答を 予測することは可能なのか -窒素応答を例に- (2019)
  • Author(s): 磯部一夫
  • Organizer: 日本土壌肥料学会
• [Presentation] Phylogenetic conservation of bacterial responses to soil nitrogen addition across continents (2018)
  • Author(s): Kazuo Isobe, Steven D Allison, Banafshe Khalili, Adam C Martiny, Jennifer BH Martiny
  • Organizer: 17 th International Symposium of Microbial Ecology
  • Int'l Joint Research
• [Presentation] Forest response to climate change: microbial ecological perspective. (2018)
  • Author(s): Kazuo Isobe
  • Organizer: 2018 NTU-UTokyo Joint Conference
  • Int'l Joint Research / Invited
• [Book] 森林学の百科事典 (2021)
  • Author(s): （一社）日本森林学会
  • Total Pages: 694
  • Publisher: 丸善出版
  • ISBN: 9784621305843
• [Book] 森林と土壌（柴田 英昭 編） (2018)
  • Author(s): 磯部 一夫
  • Total Pages: 50
  • Publisher: 共立出版
  • ISBN: 9784320058231