Chemical talking between white rot fungi and co-existing bacteria -identification of signaling chemicals and improvement of fungal functions-

• Project/Area Number: 18H02257
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 40020:Wood science-related
• Research Institution: University of Miyazaki
• Principal Investigator: Kamei Ichiro 宮崎大学, 農学部, 教授 (90526526)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2021: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2018: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: 白色腐朽菌 / 細菌 / 木材腐朽 / 微生物間相互作用 / シグナル物質 / 複合微生物系 / バイオレメディエーション / 共培養 / 共存細菌 / 菌糸伸長促進物質 / ケミカルコミュニケーション / バイオリファイナリー / バイオマス / シグナル分子 / 環境浄化 / 木質バイオリファイナリー / リグニン

Outline of Final Research Achievements

Curtobacterium sp. strain TN4W-19 which was a bacterium isolated from rotting-wood had the ability to enhance the growth of white rot fungus Stereum sp. TN4F. It was proved that ethyl acetate extract from the culture with bacterial strain TN4W-19 enhance the growth of white rot fungal strain TN4F. Then It was shown that three metabolites enhance the growth of mycelial growth of fungal strain TN4F. These results indicated that bacterium TN4W-19 enhance the growth of white rot fungus TN4F via bacterial metabolite.
The efect of co-culturing white-rot fungus Phlebia brevispora with growth-promoting bacterial strains Enterobacter sp. TN3W-14 and Pseudomonas sp. TN3W-8 on the degradation of polycyclic aromatic hydrocarbons (PAHs) was evaluated in liquid culture. The low level of degradation ability of fungal axenic culture toward benzo(a)pyrene was improved signifcantly by co-culturing the fungi with a mixture of bacterial strains TN3W-8 and TN3W-14 (mixed bacterial co-culture; MBC).

Academic Significance and Societal Importance of the Research Achievements

白色腐朽菌は木材腐朽能力に起因する高い有機物分解能力で、有害化合物の分解浄化やバイオマスの有価物への変換への応用が期待されている。本研究では、白色腐朽菌の成長を促進する要因の一つとして細菌が生産する化合物が関与することを初めて示したため学術的に新規性が高い。また、細菌との共培養により白色腐朽菌の機能を向上させることに成功した。これは、白色腐朽菌の応用研究において細菌との共培養という新しいツールを提供する点で意義がある。

Research Products

• [Int'l Joint Research] Department of Chemistry/Faculty of Science/Institut Teknologi Sepuluh Nopember(インドネシア)
• [Int'l Joint Research] Faculty of Environment/Ha Long University(ベトナム)
• [Journal Article] Interaction and Effects of Bacteria Addition on Dichlorodiphenyltrichloroethane Biodegradation by Daedalea dickinsii (2021)
  • Author(s): Rizqi Hamdan Dwi、Purnomo Adi Setyo、Kamei Ichiro
  • Journal Title: Current Microbiology Volume: 78 Issue: 2 Pages: 668-678
  • DOI: 10.1007/s00284-020-02305-8
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 微生物反応のみでバイオマス変換を完結する (2021)
  • Author(s): 亀井一郎
  • Journal Title: 化学と生物 Volume: 59 Pages: 137-143
  • Peer Reviewed
• [Journal Article] Conservation of Xylose Fermentability in Phlebia Species and Direct Fermentation of Xylan by Selected Fungi (2020)
  • Author(s): Kamei Ichiro、Uchida Kana、Ardianti Virginia
  • Journal Title: Applied Biochemistry and Biotechnology Volume: 192 Issue: 3 Pages: 895-909
  • DOI: 10.1007/s12010-020-03375-x
  • Peer Reviewed
• [Journal Article] Butanol production from cellulosic material by anaerobic co-culture of white-rot fungus Phlebia and bacterium Clostridium in consolidated bioprocessing (2020)
  • Author(s): Tri Chu Luong、Kamei Ichiro
  • Journal Title: Bioresource Technology Volume: 305 Pages: 123065-123065
  • DOI: 10.1016/j.biortech.2020.123065
  • Peer Reviewed
• [Journal Article] Synergistic interaction of a consortium of the brown-rot fungus Fomitopsis pinicola and the bacterium Ralstonia pickettii for DDT biodegradation (2020)
  • Author(s): Purnomo Adi Setyo、Sariwati Atmira、Kamei Ichiro
  • Journal Title: Heliyon Volume: 6 Issue: 6 Pages: e04027-e04027
  • DOI: 10.1016/j.heliyon.2020.e04027
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ralstonia pickettii enhance the DDT biodegradation by Pleurotus eryngii (2019)
  • Author(s): Purnomo Adi Setyo、Maulianawati Diana、Kamei Ichiro
  • Journal Title: Journal of Microbiology and Biotechnology Volume: 29 Issue: 9 Pages: 1424-1433
  • DOI: 10.4014/jmb.1906.06030
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Indirect bacterial effect enhanced less recovery of neonicotinoids by improved activities of white-rot fungus Phlebia brevispora (2019)
  • Author(s): Harry-asobara Joy L, Kamei Ichiro
  • Journal Title: Journal of Microbiology and Biotechnology Volume: 印刷中 Issue: 5 Pages: 809-812
  • DOI: 10.4014/jmb.1809.09051
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Growth management of white-rot fungus Phlebia brevispora improved degradation of high-molecular-weight polycyclic aromatic hydrocarbons (2019)
  • Author(s): Harry-asobara Joy L.、Kamei Ichiro
  • Journal Title: 3 Biotech Volume: 9 Issue: 11
  • DOI: 10.1007/s13205-019-1932-0
  • Peer Reviewed
• [Journal Article] Characteristics of White-rot Fungus Phlebia brevispora TMIC33929 and Its Growth-Promoting Bacterium Enterobacter sp. TN3W-14 in the Decolorization of Dye-Contaminated Water (2019)
  • Author(s): Harry-asobara Joy L.、Kamei Ichiro
  • Journal Title: Applied Biochemistry and Biotechnology Volume: 189 Issue: 4 Pages: 1183-1194
  • DOI: 10.1007/s12010-019-03062-6
  • Peer Reviewed / Open Access
• [Journal Article] Bacterial strains isolated from cedar wood improve the mycelial growth and morphology of white rot fungus Phlebia brevispora on agar and liquid medium (2018)
  • Author(s): Harry-asobara Joy L.、Kamei Ichiro
  • Journal Title: Journal of Wood Science Volume: 64 Issue: 4 Pages: 444-450
  • DOI: 10.1007/s10086-018-1723-y
  • NAID: 40021638207
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effects of bacterium Ralstonia pickettii addition on DDT biodegradation by Daedalea dickinsii (2018)
  • Author(s): Adi Setyo Purnomo, Hamdan Dwi Rizqi, Sri Fatmawati, Herdayanto Sulistryo Putro and Ichiro Kamei
  • Journal Title: Research Journal of Chemistry and Environment Volume: 22 Pages: 151-156
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The improvement of sodium hydroxide pretreatment in bioethanol production from Japanese bamboo Phyllostachys edulis using the white rot fungus Phlebia sp. MG-60 (2018)
  • Author(s): Taichi Motoda, Megumi Yamaguchi, Taku Tsuyama, Ichiro Kamei
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 127 Issue: 1 Pages: 66-72
  • DOI: 10.1016/j.jbiosc.2018.06.017
  • Peer Reviewed
• [Journal Article] The improvement of sodium hydroxide pretreatment in bioethanol production from Japanese bamboo Phyllostachys edulis using the white rot fungus Phlebia sp. MG-60 (2018)
  • Author(s): Chu Luong Tri, Le Duy Khuong, Ichiro Kamei
  • Journal Title: International Biodeterioration & Biodegradation Volume: 133 Pages: 86-92
  • DOI: 10.1016/j.ibiod.2018.06.010
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 白色腐朽菌 Phlebia brevispora と共存細菌 Enterobacter sp.との接触が 誘導する菌糸伸長促進効果と細菌の移動 (2021)
  • Author(s): 下川 洋太，亀井 一郎
  • Organizer: 第71回日本木材学会大会
• [Presentation] 木材蒸気式乾燥廃液からのセクイリン C およびアガサレジノールの回収 (2021)
  • Author(s): 長田 健吾，菅本 和寛，亀井 一郎
  • Organizer: 第71回日本木材学会大会
• [Presentation] 耐塩性白色腐朽菌 Phlebia sp. MG-60 株の塩ストレス応答時における MAP キナーゼ HOG1 の役割 (2021)
  • Author(s): 元田 多一，亀井 一郎
  • Organizer: 第71回日本木材学会大会
• [Presentation] Diversity of bacterial communities on maitake cultivated log (2021)
  • Author(s): FU-CHIA CHEN, Ichiro Kamei
  • Organizer: 第71回日本木材学会大会
• [Presentation] Phlebia属担子菌と共存細菌Enterobacter sp. TN3W-14との 接触が誘導する菌糸伸長促進効果と細菌の移動 (2020)
  • Author(s): 下川洋太、亀井一郎
  • Organizer: 第70回日本木材学会大会
• [Presentation] エタノール発酵性担子菌Phlebia sp. MG-60とABE発酵性細菌Clostridium saccharoperbutylacetonicum NBRC109357株の共培養によるコナラ木粉の直接ブタノール発酵 (2020)
  • Author(s): 野村駿介、Chu Luong Tri, 亀井一郎
  • Organizer: 第70回日本木材学会大会
• [Presentation] 白色腐朽菌の新機能探索－代謝改変と複合微生物による新展開－ (2019)
  • Author(s): 亀井一郎
  • Organizer: 木質科学シンポジウム
  • Invited
• [Presentation] Grifola gargal におけるベンズアルデヒド生合成経路の解明に向けたトレーサー実験 (2019)
  • Author(s): 中磨怜那、原田栄津子、亀井一郎
  • Organizer: 第26回日本木材学会九州支部大会
• [Presentation] 共存細菌 Curtobacterium sp. TN4W-19 株が生産する、Stereum 属白色腐朽菌の菌糸伸長促進物質の解明 (2019)
  • Author(s): 小関直人、亀井一郎
  • Organizer: 第26回日本木材学会九州支部大会
• [Presentation] エタノール発酵性担子菌 Phlebia sp. MG-60 P2 株と ABE 発酵性細菌 Clostridium saccharoperbutylacetonicum NBRC109357 株との共培養 (2019)
  • Author(s): 野村駿介、Chu Luong Tri、亀井一郎
  • Organizer: 第26回日本木材学会九州支部大会
• [Presentation] Direct bio-butanol production from lignocellulosic material by the co-cultivation of white rot fungus and bacterium (2019)
  • Author(s): Chu Luong Tri, Ichiro Kamei
  • Organizer: 1st International Lignin Symposium in Sapporo
  • Int'l Joint Research
• [Presentation] Down-regulation of pyruvate decarboxylase gene of white-rot fungus Phlebia sp. MG-60 modify the productivity of extracellular peroxidase activity (2019)
  • Author(s): Taichi Motoda, Ichiro Kamei
  • Organizer: 1st International Lignin Symposium in Sapporo
  • Int'l Joint Research
• [Presentation] Direct bio-butanol production from lignocellulosic material by the co-cultivation of white rot fungus and bacterium (2019)
  • Author(s): Chu Luong Tri, Ichiro Kamei
  • Organizer: The 14th Asian Biohydrogen, Biorefinery and Bioprocess Symposium
  • Int'l Joint Research
• [Presentation] 共存細菌Curtobacterium sp. TN4W-19株が生産する、白色腐朽菌Stereum sp. TN4F株の菌糸伸長促進物質の探索 (2018)
  • Author(s): 小関 直人，亀井 一郎
  • Organizer: 第69回日本木材学会大会
• [Presentation] 白色腐朽菌のエタノール発酵能抑制がもたらす木材腐朽度の低下及び腐朽材組織観察 (2018)
  • Author(s): 加藤 優美子，元田 多一，津山 濯，雉子谷 佳男，亀井 一郎
  • Organizer: 第69回日本木材学会大会
• [Presentation] 水分環境の変化が白色腐朽菌Phlebia sp. MG-60株の木質分解関連遺伝子および浸透圧調節関連遺伝子の発現に与える影響 (2018)
  • Author(s): 元田 多一，亀井 一郎
  • Organizer: 第69回日本木材学会大会
• [Presentation] 耐塩性白色腐朽菌Phlebia sp. Strain MG-60が生産するManganese peroxidaseの特性比較 (2018)
  • Author(s): 富高 奈南，山崎 有美，亀井 一郎
  • Organizer: 第69回日本木材学会大会
• [Presentation] 木材腐朽時に白色腐朽菌カワラタケが分泌する細胞外小胞の分画と酵素活性 (2018)
  • Author(s): 大塚晃生、津山濯、亀井一郎
  • Organizer: 第25回日本木材学会九州支部大会
• [Presentation] 白色腐朽菌Phlebia sp. MG-60 P2株のエタノール発酵能抑制が木片腐朽に与える影響 (2018)
  • Author(s): 加藤優美子、元田多一、亀井一郎
  • Organizer: 第25回日本木材学会九州支部大会
• [Presentation] Phlebia属担子菌と共生細菌Enterobacter sp. TN3W-14との接触による菌糸伸長促進効果と細菌の移動 (2018)
  • Author(s): 下川洋太、亀井一郎
  • Organizer: 第25回日本木材学会九州支部大会
• [Presentation] 木材の主成分及びコナラ（Quercus serrata）の抽出成分が白色腐朽菌Stereum sp. TN4F株の菌糸伸長に与える影響 (2018)
  • Author(s): 小関直人、亀井一郎
  • Organizer: 第25回日本木材学会九州支部大会
• [Presentation] 単糖蓄積性白色腐朽菌と ABE 発酵性細菌の共培養によるセルロースの直接ブタノール発酵 (2018)
  • Author(s): 溝口佑真、亀井一郎
  • Organizer: 第25回日本木材学会九州支部大会
• [Presentation] POPs分解性菌類とその分解代謝経路 (2018)
  • Author(s): 亀井一郎
  • Organizer: 第36回農薬環境科学研究会シンポジウム
  • Invited
• [Presentation] Molecular breeding of white rot fungus Phlebia sp. MG-60 to convert lignocellulose materials (2018)
  • Author(s): Ichiro Kamei, Taichi Motoda, Taku Tsuyama
  • Organizer: Lignobiotech 2018
  • Int'l Joint Research
• [Presentation] Cloning and expression analysis of aquaporins in white-rot fungus Phlebia sp. MG-60 (2018)
  • Author(s): Taichi Motoda, Ichiro Kamei
  • Organizer: International Seminar on Chemistry 2018
  • Int'l Joint Research
• [Presentation] Integrated fungal fermentation for the conversion of woody biomass by the multifunctional fungus (2018)
  • Author(s): Ｉｃｈｉｒｏ Ｋａｍｅｉ
  • Organizer: International Seminar on Chemistry 2018
  • Int'l Joint Research / Invited
• [Presentation] The effect of different xylan in the ethanol production by the white-rot fungi Phlebia genera species (2018)
  • Author(s): Virginia Ardianti, Kana Uchida, Ichiro Kamei
  • Organizer: International Seminar on Chemistry 2018
  • Int'l Joint Research
• [Book] Fungi in fuel biotechnology (Chapter 6 Wood-Rotting Fungi for Biofuel Production) (2020)
  • Author(s): Ichiro Kamei
  • Total Pages: 25
  • Publisher: Springer
  • ISBN: 9783030444877
• [Remarks] 森林緑地研究の最前線
  • URL: http://www.miyazaki-u.ac.jp/green/member/kamei.pdf
• [Remarks] 農学図鑑
  • URL: http://www.miyazaki-u.ac.jp/agr/books/post-27.html
• [Remarks] 森林バイオマス科学研究室
  • URL: https://www.cc.miyazaki-u.ac.jp/kamei/index.html
• [Patent(Industrial Property Rights)] 発光きのこおよび発光菌糸体の栽培方法 (2019)
  • Inventor(s): 亀井一郎、原田栄津子
  • Industrial Property Rights Holder: 亀井一郎、原田栄津子
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2019-153252
  • Filing Date: 2019
• [Patent(Industrial Property Rights)] 木材乾燥廃液から得られる金属補給体並びに木材乾燥廃液の脱色方法及び木材心材成分の回収方法 (2019)
  • Inventor(s): 亀井一郎
  • Industrial Property Rights Holder: 亀井一郎
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2019-146938
  • Filing Date: 2019