Revisiting the hypothesis of steroid-induced sex determination in fish

• Project/Area Number: 18H02277
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 40040:Aquatic life science-related
• Research Institution: The University of Tokyo
• Principal Investigator: Kikuchi Kiyoshi 東京大学, 大学院農学生命科学研究科(農学部), 教授 (20292790)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2018: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: 性決定遺伝子 / 水産育種 / 性決定 / 魚類 / 内分泌

Outline of Final Research Achievements

Sex-determining genes can be used for sex identification, providing critical information for genetic breeding in aquaculture. Since identifying them is still challenging for most fish, we are exploring strategies using multiple approaches. In this study, we show strong evidence that the combination of Hsd17b1 alleles determines sex by modulating endogenous estrogen levels in three Seriola species. Our findings strongly support the hypothesis of steroid-induced sex determination in fish, which has been introduced more than 50 years ago but has never been rigorously tested.

Academic Significance and Societal Importance of the Research Achievements

本研究では、ブリ類3種で利用可能な性判別法を開発することに成功した。ブリ類3種が日本の海面養殖生産量の過半数を占めていること、さらに近年、ブリ類の養殖と品種改良が欧州・豪州・南北米で活発化していることを考慮すると、この性判別マーカーの開発は、グローバルな水産養殖の文脈で大きな意義をもつ可能性がある。また本研究により、「性ホルモンが性決定の最初のトリガーを引く」生物が存在することが明確に示された。この結果は、半世紀以上前に提唱されていたにも関わらず、決定的な証拠が欠けていた「性決定のステロイド誘導説」を強く支持するもであり、その学術上の意義は大きい。

Research Products

• [Journal Article] Precocious Maturation in Male Tiger Pufferfish Takifugu rubripes: Genetics and Endocrinology. (2020)
  • Author(s): Yoshikawa S., Chuda H., Hamasaki M., Kadomura K., Yamada T., Kikuchi K. Hosoya S.
  • Journal Title: Fisheries Science Volume: 86 Issue: 2 Pages: 339-351
  • DOI: 10.1007/s12562-019-01390-4
  • NAID: 40022196065
  • Peer Reviewed
• [Journal Article] Ⅰ-2. Sex-determining genes in <i>Takifugu</i> pufferfishes (2019)
  • Author(s): KIKUCHI KIYOSHI、IEDA RISA、FUJIKAWA DAIGAKU、AHAMMAD KABIR、KOYAMA TAKASHI、HOSOYA SHO
  • Journal Title: NIPPON SUISAN GAKKAISHI Volume: 85 Issue: 2 Pages: 187-187
  • DOI: 10.2331/suisan.WA2603-3
  • NAID: 130007622996
  • ISSN: 0021-5392, 1349-998X
  • Year and Date: 2019-03-15
  • Open Access
• [Journal Article] Ⅰ-3. Sex Determination Mechanism in <i>Seriola</i> Fishes (2019)
  • Author(s): KOYAMA TAKASHI、NAKAMOTO MASATOSHI、MORISHIMA KAGAYAKI、YAMASHITA TAKEFUMI、MIZUNO NAOKI、KIKUCHI KIYOSHI、SAKAMOTO TAKASHI
  • Journal Title: NIPPON SUISAN GAKKAISHI Volume: 85 Issue: 2 Pages: 188-188
  • DOI: 10.2331/suisan.WA2603-4
  • NAID: 130007622995
  • ISSN: 0021-5392, 1349-998X
  • Year and Date: 2019-03-15
  • Open Access
• [Journal Article] Foreword (2019)
  • Author(s): 菊池潔, 井尻成保, 北野健
  • Journal Title: NIPPON SUISAN GAKKAISHI Volume: 85 Issue: 2 Pages: 185-185
  • DOI: 10.2331/suisan.WA2603-1
  • NAID: 130007622992
  • ISSN: 0021-5392, 1349-998X
  • Year and Date: 2019-03-15
  • Open Access
• [Journal Article] A SNP in a Steroidogenic Enzyme Is Associated with Phenotypic Sex in Seriola Fishes (2019)
  • Author(s): T. Koyama, M. Nakamoto#, K. Morishima#, R. Yamashita#, T. Yamashita#, K. Sasaki, Y. Kuruma, N. Mizuno, M. Suzuki, K. Okada, R. Ieda, T. Uchino, S. Tasumi, S. Hosoya, S. Uno, J. Koyama, A. Toyoda, K. Kikuchi, T. Sakamoto
  • Journal Title: Current Biology Volume: 29 Issue: 11 Pages: 1901-1909
  • DOI: 10.1016/j.cub.2019.04.069
  • Peer Reviewed / Open Access
• [Journal Article] Random PCR-based genotyping by sequencing technology GRAS-Di reveals genetic structure of mangrove fishes (2019)
  • Author(s): Sho Hosoya, Shotaro Hirase, Kiyoshi Kikuchi, Kusuto Nanjo, Yohei Nakamura, Hiroyoshi Kohno, Mitsuhiko Sano
  • Journal Title: Molecular Ecology Resources Volume: 19 Issue: 5 Pages: 1153-1163
  • DOI: 10.1111/1755-0998.13025
  • Peer Reviewed
• [Journal Article] A highly flexible and repeatable genotyping method for aquaculture studies based on target amplicon sequencing using next-generation sequencing technology (2019)
  • Author(s): Sato Mana、Hosoya Sho、Yoshikawa Sota、Ohki Shun、Kobayashi Yuki、Itou Takuya、Kikuchi Kiyoshi
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 6904-6904
  • DOI: 10.1038/s41598-019-43336-x
  • Peer Reviewed / Open Access
• [Journal Article] The Genetic Basis of Scale-Loss Phenotype in the Rapid Radiation of Takifugu Fishes (2019)
  • Author(s): Dong In Kim, Wataru Kai, Sho Hosoya, Mana Sato, Aoi Nozawa, Miwa Kuroyanagi, Yuka Jo, Satoshi Tasumi, Hiroaki Suetake, Yuzuru Suzuki, Kiyoshi Kikuchi
  • Journal Title: Genes Volume: 10 Issue: 12 Pages: 1027-1027
  • DOI: 10.3390/genes10121027
  • Peer Reviewed / Open Access
• [Journal Article] Identification of the sex-determining locus in grass puffer (Takifugu niphobles) provides evidence for sex-chromosome turnover in a subset of Takifugu species (2018)
  • Author(s): Risa Ieda, Sho Hosoya, Shota Tajima, Kazufumi Atsumi, Takashi Kamiya, Aoi Nozawa, Yuma Aoki, Satoshi Tasumi, Takashi Koyama, Osamu Nakamura, Yuzuru Suzuki, Kiyoshi Kikuchi
  • Journal Title: PLOSONE Volume: 13 Issue: 1 Pages: e0190635-e0190635
  • DOI: 10.1371/journal.pone.0190635
  • Peer Reviewed / Open Access
• [Presentation] 超多検体トランスクリプトーム解析を志向したライブラリ作製法の立ち上げ (2020)
  • Author(s): 小山喬・菊池潔・豊田敦
  • Organizer: 平成32年度日本水産学会春季大会
• [Presentation] マイクロ流路チップを用いた水産生物の細胞解析 (2020)
  • Author(s): 小祝敬一郎・村上友樹・鈴木宏明・小山喬・菊池潔・ 豊田敦・津田宗一郎
  • Organizer: 平成32年度日本水産学会春季大会
• [Presentation] Genomic identification and characterization of sex-determining locus of Takifugu niphobles by a chromosome scale genome assembly (2020)
  • Author(s): Kabir Ahammad, Risa Ieda, Daigaku Fujikawa, Shotaro Hirase, Takashi Koyama, Atsushi Toyota, Shigehiro Kuraku, Sho Hosoya, Kiyoshi Kikuchi
  • Organizer: 平成32年度日本水産学会春季大会
• [Presentation] ブリ属3種の比較ゲノム解析 (2020)
  • Author(s): 車遥介・小山喬・菊池潔・森島輝・小林久人・志波優
  • Organizer: 平成32年度日本水産学会春季大会
• [Presentation] NGSによる性染色体同定法とステロイド依存型の性決定機構について (2019)
  • Author(s): 菊池潔
  • Organizer: DMYの会
• [Presentation] ブリ類の初期性分化メカニズム解明を目指したscRNA-seqの活用 (2019)
  • Author(s): 小山喬・小祝敬一郎・津田宗一郎・菊池潔
  • Organizer: シングルセル研究会
• [Presentation] Revisiting steroids as natural inducers of sex determination: A SNP in a steroidogenic enzyme is associated with phenotypic sex in Seriola fishes (2019)
  • Author(s): Kiyoshi Kikuchi
  • Organizer: Asian Sex Differentiation Network (8th Gonad Biology Joint Meeting, 2019)
  • Int'l Joint Research / Invited
• [Presentation] ブリ属の性決定領域ゲノム配列比較 (2019)
  • Author(s): 小山喬・車遥介・菊池潔・森島輝・豊田敦
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] 半数体ブリゲノムを用いた性拮抗遺伝子の探索 (2019)
  • Author(s): 車遥介・小山喬・菊池潔・森島輝・小林久人
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] トラフグ白子早熟形質のQTL 解析 (2019)
  • Author(s): 吉川壮太・濱崎将臣・山田敏之・中田久・細谷将・菊池潔
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] ブリ属ステロイド代謝関連遺伝子の発現解析 (2019)
  • Author(s): 車遥介・小山喬・菊池潔・中本正俊・坂本崇
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] カンパチ性分化関連遺伝子の網羅的探索 (2019)
  • Author(s): 小山喬・菊池潔
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] Single-Molecule sequencing has resolved the complex genomic region of the sex-determining locus in Kusafugu Takifugu niphobles (2019)
  • Author(s): Kabir Ahammad・Risa Ieda・Daigaku Fujikawa・Mana Sato・Shotaro Hirase・Takashi Koyama・Atsushi Toyota・Sho Hosoya・Kiyoshi Kikuchi
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] クサフグで生じた性的拮抗の解消 (2019)
  • Author(s): 藤川大学・家田梨櫻・Kabir Ahammad・小山喬・細谷将・菊池潔
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] 半数体ゲノムのマルチプレックスシーケンシング－ FSTを指標とした性拮抗遺伝子の探索 － (2019)
  • Author(s): 小山喬・車遥介・菊池潔・小林久人
  • Organizer: 東京農業大学生物資源ゲノム解析拠点シンポジウム
• [Presentation] トラフグ属魚類の性決定遺伝子と魚類育種素材としての野生魚の利用可能性 (2019)
  • Author(s): 細谷将・小林久人・菊池潔
  • Organizer: 東京農業大学生物資源ゲノム解析拠点シンポジウム
• [Presentation] 魚類における性染色体のターンオーバー (2019)
  • Author(s): Kabir Ahammad・藤川大学・家田梨櫻・小林久人・豊田敦・細谷将・菊池潔
  • Organizer: 東京農業大学生物資源ゲノム解析拠点シンポジウム
• [Presentation] The genetic and steroidogenic sex determination in Seriola fishes (2018)
  • Author(s): Takashi Koyama, Masatoshi Nakamoto, Kagayaki Morishima, Ryohei Yamashita, Takefumi Yamashita, Kohei Sasaki, Naoki Mizuno, Yosuke Kuruma, Moe Suzuki, Risa Ieda, Tsubasa Uchino, Satoshi Tasumi, Sho Hosoya, Seiichi Uno, Jiro Koyama, Atsushi Toyoda, Kiyoshi Kikuchi, Takashi Sakamoto
  • Organizer: 日本分子生物学会第41回（2018年）年会
  • Invited
• [Presentation] フグ類の性決定遺伝子たち (2018)
  • Author(s): 菊池潔
  • Organizer: 平成３０年度日本水産学会秋季大会
• [Presentation] Turnover of sex chromosomes in Takifugu fishes (2018)
  • Author(s): Ieda R, Tajima S, Atsumi K, Fujikawa D, Sato M, Ahammad K, Nozawa A, Koyama T, Toyoda A, Kobayashi H, Tasumi S, Hosoya S, Kikuchi K
  • Organizer: The 2018 International Symposium of Genetics in Aquaculture
  • Int'l Joint Research
• [Presentation] Trans-species SNP in a steroidogenic gene is associated with sex determination in Seriola species (2018)
  • Author(s): Koyama T, Nakamoto M, Morishima K, Yamashita R, Yamashita T, Sasaki K, Suzuki M, Kuruma Y, Ieda R, Uchino T, Tasumi S, Hosoya S, Uno S, Koyama J, Toyoda A, Kikuchi K, Sakamoto T
  • Organizer: The 2018 International Symposium of Genetics in Aquaculture
  • Int'l Joint Research
• [Presentation] Revisiting the steroid theory of sex inducer; Trans-species SNP in a steroidogenic enzyme, Hsd17b1, is associated with sex in Seriola species. (2018)
  • Author(s): Takashi Koyama, Masatoshi Nakamoto, Kagayaki Morishima, Ryohei Yamashita, Takefumi Yamashita, Sasaki Kohei, Naoki Mizuno, Moe Suzuki, Risa Ieda, Tsubasa Uchino, Satoshi Tasumi, Sho Hosoya, Seiichi Uno, Jiro Koyama, Atsushi Toyoda, Takashi Sakamoto, Kiyoshi Kikuchi
  • Organizer: Eight International Symposium on Vertebrate Sex Determination
  • Int'l Joint Research
• [Presentation] Turnover of sex chromosomes in Takifugu pufferfishes (2018)
  • Author(s): Risa Ieda, Shota Tajima, Kazufumi Atsumi, Daigaku Fujikawa, Mana Sato, Yosuke Kuruma, Shun Ohki, Aoi Nozawa, Takashi Koyama, Satoshi Tasumi, Sho Hosoya, Kiyoshi Kikuchi
  • Organizer: Eight International Symposium on Vertebrate Sex Determination
  • Int'l Joint Research
• [Remarks] ブリ類の性決定遺伝子を発見
  • URL: https://www.a.u-tokyo.ac.jp/topics/topics_20190605-1.html
• [Remarks] 東京大学大学院農学生命科学研究科 附属水産実験所
  • URL: http://www.se.a.u-tokyo.ac.jp/japanese.html
• [Patent(Industrial Property Rights)] ブリ類の性別識別方法 (2018)
  • Inventor(s): 坂本崇、中本正俊、菊池潔、小山喬
  • Industrial Property Rights Holder: 坂本崇、中本正俊、菊池潔、小山喬
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2018-027260
  • Filing Date: 2018