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Creation of novel plant-microbe hybrid remediation system by a POPs-degrading endophytic bacteria and Cucurbitaceae plant.

Research Project

Project/Area Number 18H02319
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 41050:Environmental agriculture-related
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

Takagi Kazuhiro  国立研究開発法人農業・食品産業技術総合研究機構, 農業環境変動研究センター, 主席研究員 (70354074)

Co-Investigator(Kenkyū-buntansha) 片岡 良太  山梨大学, 大学院総合研究部, 助教 (00635104)
清田 洋正  岡山大学, 環境生命科学研究科, 教授 (30234397)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords植物内生菌 / 新規PCP分解菌 / 新規分解代謝物 / 単結晶X線構造解析法 / 残留性有機汚染物質(POPs) / 汚染土壌修復 / 内生分解細菌 / 植物・微生物複合系 / ウリ科植物 / DDT、PCP / POPs汚染土壌修復 / 有機塩素系殺虫剤 / 残留生有機汚染物質 / 新規DDT分解菌 / 分解代謝物 / 環境修復 / DDT, PCP / 新規DDT分解細菌 / 分解代謝経路 / 分解酵素遺伝子 / DDT,PCP
Outline of Final Research Achievements

We tried the approach from the academic question, “Does it possible for remediation by constructing a plant-microbial complex system?” Cucurbitaceae plants that specifically uptake POPs were used in this study, and we revealed endophytic bacterial community in Cucurbitaceae plants. On the other hand, although we explored DDT and PCP-degrading bacteria from the endophytic bacteria living in Cucurbitaceae plants, unfortunately we could not find any DDT-degrading bacteria. However, we succeeded in identifying PCP-degrading bacteria, and it was clarified that PCP was converted to more hydrophilic unknown metabolite. We identified the unknown metabolite as PCP-phosphate using an advanced single crystal X-ray diffraction measurement. PCP phosphate is a novel metabolite and terminal compound. There have been no reports of POPs degradation by endophytic bacteria to date, and this is the first achievement all over the world.

Academic Significance and Societal Importance of the Research Achievements

本研究を通して、植物-微生物複合系構築によるレメディエーションは可能か?という当初の問いかけに対して回答し得る一定の成果を得た。つまり、植物の内部にはヒトの腸内と同じように数多くの微生物が生息しており、その中にはPOPsのような難分解性有機化合物を分解できる微生物も存在していることが明らかとなった。これにより、これまで課題とされてきた分解菌を汚染現場に定着させ安定的に機能を維持させるという難題を、分解菌を接種し保持させた植物を汚染現場に適応することで解決できる可能性がある。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (11 results)

All 2021 2020 2019

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (6 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Dichlorodiphenyltrichloroethane (DDT) degradation by Streptomyces sp. isolated from DDT contaminated soil2021

    • Author(s)
      Ito Koji、Mahmood Ahmad、Kataoka Ryota、Takagi Kazuhiro
    • Journal Title

      Bioremediation Journal

      Volume: 25 Issue: 2 Pages: 1-14

    • DOI

      10.1080/10889868.2020.1867050

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Study on the biodegradation of persistent organic pollutants (POPs)2020

    • Author(s)
      Kazuhiro Takagi
    • Journal Title

      Journal of Pesticide Science

      Volume: 45 Issue: 2 Pages: 119-123

    • DOI

      10.1584/jpestics.J19-06

    • NAID

      130007844649

    • ISSN
      1348-589X, 1349-0923
    • Year and Date
      2020-05-20
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biochemical characterization of NADH:FMN oxidoreductase HcbA3 from <i>Nocardioides</i> sp. PD653 in catalyzing aerobic HCB dechlorination2020

    • Author(s)
      Ito Koji、Takagi Kazuhiro、Kataoka Ryota、Kiyota Hiromasa
    • Journal Title

      Journal of Pesticide Science

      Volume: 45 Issue: 3 Pages: 125-131

    • DOI

      10.1584/jpestics.d20-23

    • NAID

      130007889122

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dissipation, dehalogenation, and denitration of chloroaromatic compounds by <i>Nocardioides</i> sp. strain PD653: Characterization of the substrate specificity2019

    • Author(s)
      Ito K., Takagi K., Kataoka R., Kiyota H., Iwasaki A.
    • Journal Title

      Journal of Pesticide Science

      Volume: 44 Issue: 3 Pages: 171-176

    • DOI

      10.1584/jpestics.D19-024

    • NAID

      130007691510

    • ISSN
      1348-589X, 1349-0923
    • Year and Date
      2019-08-20
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in Endophytic Bacterial Communities During Different Growth Stages of Cucumber (Cucumis sativus L.)2019

    • Author(s)
      Mahmood A., Takagi K., Ito K., Kataoka R.
    • Journal Title

      World Journal of Microbiology and Biotechnology

      Volume: 35 Issue: 7 Pages: 104-104

    • DOI

      10.1007/s11274-019-2676-z

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] 放線菌PD653株によるヘキサクロロベンゼンおよびペンタクロロフェノールの好気的脱塩素機構に関する研究2021

    • Author(s)
      伊藤虹児
    • Organizer
      日本農薬学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 好気的HCB脱塩素反応に関与するフラビンリダクターゼHcbA3の生化学的解析2020

    • Author(s)
      伊藤 虹児, 髙木 和広, 片岡 良太, 清田 洋正
    • Organizer
      日本農薬学会第45回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Temporal Endophytic Bacterial Dynamics of Cucumber; A Culture Dependent and Independent Approach2019

    • Author(s)
      Mahmood A., Takagi K., Ito K., Kataoka R.
    • Organizer
      International Soil Congress in Turkey
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The substrate specificity of Nocardioides sp. PD653 capable of degrading hexachlorobenzene.2019

    • Author(s)
      Ito K.., Takagi K., Kataoka R., Kiyota H.
    • Organizer
      XIX International Plant Protection Congress(IPPC 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bioremediation of hexachlorocyclohexanes (HCHs)-contaminated soil using charcoal enriched with a constructed bacterial consortium.2019

    • Author(s)
      Takagi K., Kataoka R., Ito K.
    • Organizer
      XIX International Plant Protection Congress(IPPC 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 好気的HCB脱塩素分解菌Nocardioides sp. PD653株の基質特異性と脱塩素酵素の精製2019

    • Author(s)
      伊藤 虹児, 髙木 和広, 片岡 良太, 清田 洋正
    • Organizer
      第37回農薬環境科学研究会
    • Related Report
      2019 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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