Methane mitigation from ruminants by wide range application of alkylpenol-containing plants

• Project/Area Number: 18H02322
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 42010:Animal production science-related
• Research Institution: Hokkaido University
• Principal Investigator: Kobayashi Yasuo 北海道大学, 農学研究院, 教授 (50153648)
• Co-Investigator(Kenkyū-buntansha): 鈴木 裕 北海道大学, 農学研究院, 助教 (10793846) ; 小池 聡 北海道大学, 農学研究院, 准教授 (90431353)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2021: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: アルキルフェノール / ギンナン果肉 / カシュー殻液 / メタン / 温室効果 / 糞便 / ルーメン / 微生物相 / スラリー / 排泄物 / メタン低減 / カシューナッツ殻液 / 温暖化

Outline of Final Research Achievements

Methane mitigation potential of alkylphenol-containing plants such as ginkgo fruit (GF) and cashew nut shell liquid (CL) was evaluated by the application as manure and/or feed additive. Considering local availability of each material, GF was supposed to use for Japanese cattle, while CL was for native cattle and swamp buffaloes in Thailand. The evaluations were made by monitoring changes of fermentation products and microbiota in in vitro culture systems and also in feeding studies.
Both materials decreased methane production potential by the direct addition to feces with significant alteration of fecal microbial community, based on the selective anti-microbial actions. CL was further evaluated by feeding studies using Thani animals and was found to decrease methane production not only from the rumen fluid but also from feces. This accompanied with apparent changes in fecal microbiota.

Academic Significance and Societal Importance of the Research Achievements

畜産分野の主たる温室効果ガスであるウシからのメタンは、その削減が喫緊の課題となっている。ウシルーメンのみならず、排泄物からのメタンも無視できない量であり、この低減も講じる必要がある。選択性の高い抗菌作用を有するアルキルフェノールはルーメンの菌叢を変えメタン低減を導くが、本研究で初めて糞便の菌叢にも影響し、メタン生成を抑制することを証明した。アルキルフェノール含有植物は稀な存在であり量的確保可能なものが限られるが、ギンナン果肉とカシュー殻液が有力候補である。ただし、分布に地域性があるため、前者は日本国内で、後者はタイで、各々代表家畜の糞便に活用する方向で評価し、有益な成果を得た。

Research Products

• [Int'l Joint Research] Kasetsart University(タイ)
• [Int'l Joint Research] Faculty of Agriculture/Kasetsart University(タイ)
• [Journal Article] Microbial community structure of the bovine rumen as affected by feeding cashew nut shell liquid, a methane‐inhibiting and propionate‐enhancing agent. (2021)
  • Author(s): Su, C., Shinkai, T., Miyazawa, N., Mitsumori, M., Enishi, O., Nagashima, K., Koike,S., Kobayashi, Y.
  • Journal Title: Animal Science Journal Volume: 92 Issue: 1
  • DOI: 10.1111/asj.13503
  • NAID: 120007181808
  • Peer Reviewed
• [Journal Article] Feeding cashew nut shell liquid decreases methane production from feces by altering fecal bacterial and archaeal communities in Thai local ruminants. (2021)
  • Author(s): Tamori, K., Matsunaga, B., Boonsaen, P., Khongpradit, A., Sawanon, S.,Nagashima, K., Koike, S., Kobayashi, Y.
  • Journal Title: Animal Science Journal Volume: 92 Issue: 1
  • DOI: 10.1111/asj.13569
  • NAID: 120007181804
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Growth and morphologic response of rumen methanogenic archaea and bacteria to cashew nut shell liquid and its alkylphenol components. (2021)
  • Author(s): Wakai, M., Hayashi, S., Chiba, Y., Koike, S., Nagashima, K., Kobayashi, Y.
  • Journal Title: Animal Science Journal Volume: 92 Issue: 1
  • DOI: 10.1111/asj.13598
  • Peer Reviewed
• [Journal Article] Addition of ginkgo fruit to cattle feces and slurry suppresses methane production by altering the microbial community structure. (2021)
  • Author(s): Shintani, R., Oh, S., Suzuki, Y., Koike, S., Kobayashi, Y.
  • Journal Title: Animal Science Journal Volume: 92 Issue: 1
  • DOI: 10.1111/asj.13620
  • Peer Reviewed
• [Journal Article] Cashew nut shell liquid potentially mitigates methane emission from the feces of Thai native ruminant livestock by modifying fecal microbiota. (2021)
  • Author(s): Narabe, C., Kamiyama, S., Saito, M., Boonsaen, P., Khongpradit, A., Sawanon, S., Suzuki , Y., Koike , S, Kobayashi , Y.
  • Journal Title: Animal Science Journal Volume: 92 Issue: 1
  • DOI: 10.1111/asj.13614
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 環境調和をみすえる家畜栄養学の展開：温暖化ガスの低減に向けて (2020)
  • Author(s): 小林泰男
  • Journal Title: Nihon Chikusan Gakkaiho Volume: 91 Issue: 3 Pages: 304-306
  • DOI: 10.2508/chikusan.91.304
  • NAID: 130007922113
  • ISSN: 1346-907X, 1880-8255
  • Year and Date: 2020-08-25
• [Journal Article] Effect of ruminal administration of soy sauce oil on rumen fermentation, milk production and blood parameters in dairy cows. (2020)
  • Author(s): Konno, D., Takahashi, M., Osaka, I,, Orihashi, T., Sakai, K., Sera, K., Obara, Y., Kobayashi, Y.
  • Journal Title: Asian-Australasian Journal of Animal Sciences Volume: 33 Issue: 11 Pages: 1779-1786
  • DOI: 10.5713/ajas.19.0617
  • Peer Reviewed / Open Access
• [Journal Article] Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle (2020)
  • Author(s): Khongpradit, A., Boonsaen, O., Homwong, N., Suzuki, Y., Koike, S., Sawanon, S., Kobayashi, Y.
  • Journal Title: Animal Science Journal Volume: 91 Issue: 1
  • DOI: 10.1111/asj.13459
  • NAID: 120007151359
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The bio‐surfactant mannosylerythritol lipid acts as a selective antibacterial agent to modulate rumen fermentation (2020)
  • Author(s): Okuhira, K., Koike, S., Ito, S., Kobayashi, Y.
  • Journal Title: Animal Science Journal Volume: 91 Issue: 1
  • DOI: 10.1111/asj.13464
  • Peer Reviewed
• [Journal Article] Seasonal differences in rumen bacterial flora of wild Hokkaido sika deer and partial characterization of an unknown bacterial group possibly involved in fiber digestion in winter (2019)
  • Author(s): Yamano, H., Ichimura, Y., Sawabe, Y., Koike, S., Suzuk, Y., Kobayashi, Y.
  • Journal Title: Animal Science Journal Volume: 90 Issue: 6 Pages: 790-798
  • DOI: 10.1111/asj.13203
  • NAID: 120006847762
  • Peer Reviewed
• [Journal Article] Effect of cashew nut shell liquid feeding on fermentation and microbiota in the rumen of Thai native cattle and swamp buffaloes (2019)
  • Author(s): Konda, S., Onodera, R., Kanchanasatit, E., Boonsaen, P., Sawanon, S., Nagashima, K., Suzuki, Y., Koike, S., Kobayashi, Y.
  • Journal Title: Livestock Science Volume: 226 Pages: 99-106
  • DOI: 10.1016/j.livsci.2019.06.011
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Selection of plant oil as a supplemental energy source by monitoring rumen profiles and its dietary application in Thai crossbred beef cattle (2019)
  • Author(s): Matsuba, K., Padlom, A., Khongpradit, A., Boonsaen, P., Thirawong, P., Sawanon, S., Suzuki, Y., Koike, S., Kobayashi, Y.
  • Journal Title: Asian-Australasian Journal of Animal Science Volume: 32 Issue: 10 Pages: 1511-1520
  • DOI: 10.5713/ajas.18.0946
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rumen responses to dietary supplementation with cashew nut shell liquid and its cessation in sheep (2018)
  • Author(s): Kang S, Suzuki R, Suzuki Y, Koike S, Nagashima K, Kobayashi Y
  • Journal Title: Animal Science Journal Volume: 89 Issue: 11 Pages: 1549-1555
  • DOI: 10.1111/asj.13100
  • NAID: 120006734168
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Rumen methane suppression toward low carbon ruminant production. (2021)
  • Author(s): Kobayashi, Y.
  • Organizer: International webinar on rumen methane suppression. Online operated by Seoul National University. September 17
  • Int'l Joint Research / Invited
• [Presentation] Mitigation of methane from cattle through controlling gut microbiota and Fermentation. (2021)
  • Author(s): Kobayashi, Y.
  • Organizer: Materials Research Meeting 2021. Pacifico Yokohama. Decmber 13.
  • Int'l Joint Research / Invited
• [Presentation] Effect of early roughage feeding on growth and hindgut environment of pre-weaned calves (2021)
  • Author(s): Heshan Kodithuwakku・大和田尚・平野和夫・三浦広卓・鈴木裕・小林泰男・小池聡
  • Organizer: 日本畜産学会第128回大会
• [Presentation] 他菌の発酵産物がルーメン細菌Selenomonas ruminantium の運動性と増殖性に及ぼす影響 (2021)
  • Author(s): 宮崎黎・鈴木裕・小林泰男・小池聡
  • Organizer: 日本畜産学会第128回大会
• [Presentation] Recent challenges for mitigation of methane emission from ruminants (2020)
  • Author(s): Yasuo Kobayashi
  • Organizer: Korean Society of Rumen Function Studies
  • Int'l Joint Research / Invited
• [Presentation] 消化管環境の制御による家畜生産性の向上と代謝障害の予防 (2019)
  • Author(s): 小林泰男
  • Organizer: 家畜感染症学会
  • Invited
• [Presentation] 第一胃微生物からみたウシの疾病と栄養制御 (2019)
  • Author(s): 小林泰男
  • Organizer: 大動物臨床研究会
  • Invited
• [Presentation] ルーメン微生物研究グループからの発表 (2018)
  • Author(s): 小林泰男
  • Organizer: 第2回畜産分野での温室効果ガス排出削減に向けた研究ネットワーク会合
  • Invited
• [Book] 畜産学概論 (2021)
  • Author(s): 小林泰男 他9名
  • Total Pages: 184
  • Publisher: 朝倉書店
  • ISBN: 9784254450316
• [Remarks] 研究テーマ１：動物機能栄養学
  • URL: https://anim-func-nutr.agr.hokudai.ac.jp/introduction/theme01/
• [Remarks]
  • URL: https://anim-func-nutr.agr.hokudai.ac.jp/
• [Remarks] 動物機能栄養学研究室
  • URL: https://www.agr.hokudai.ac.jp/r/lab/animal-function-and-nutrition?from=us