Development of a method to control poultry red mites using recombinant Marek's disease virus that secretes a vaccine antigen

• Project/Area Number: 18H02332
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Hokkaido University
• Principal Investigator: Murata Shiro 北海道大学, 獣医学研究院, 助教 (10579163)
• Co-Investigator(Kenkyū-buntansha): 今内 覚 北海道大学, 獣医学研究院, 准教授 (40396304) ; 大橋 和彦 北海道大学, 獣医学研究院, 教授 (90250498)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000); Fiscal Year 2020: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: ワクモ / 外部寄生虫 / マレック病 / マレック病ウイルス / 組換えウイルス / 組換えワクチン / 弱毒生ワクチン / ウイルスベクター

Outline of Final Research Achievements

Poultry red mites (PRMs, Dermanyssus gallinae) are haematophagous ectoparasites which causes serious economic losses to the poultry industry worldwide, and the development of novel methods to control PRMs is required. We have investigated the application of vaccination and have shown that immunization with vaccine antigens could contribute to the reduction in the number of PRMs. In addition, we investigate the application of virus vectors to develop easy-to-use vaccines for farmers. In this study, we designed the recombinant virus that secrets vaccine antigens to improve the efficiency of vaccines using the recombinant virus. To produce an antigen-secreting virus, we inserted the signal peptides of a virus-derived secretory protein to the virus genome. However, the secretion of antigens was not confirmed, although the expression was observed in infected cells. Further manipulation is required to generate effective vaccines using virus vectors for controlling PRMs.

Academic Significance and Societal Importance of the Research Achievements

抗ワクモワクチンの実用化に向け、組換えタンパク質をワクチン抗原として用いる場合、アジュバントの添加や、複数回ワクチン接種を行う必要があり、鶏への負荷や飼養者への負担（時間やコスト等）の増加が懸念される。本研究ではウイルスベクターを応用・改良することで、効果の高い組換えウイルスを用いた抗ワクモワクチンの作製を試みた。今回の結果では、感染細胞からのワクチン抗原の分泌の確認までは至らなかった。しかし本研究の結果を基盤として、
プロモーターの改変や抗原遺伝子の挿入部位などのさらなる改良を加えることで、飼養者への負担を軽減させ、効果の高いワクモ防除用ワクチン開発に貢献すると考えられる。

Research Products

• [Journal Article] Selection of reference genes for quantitative PCR analysis in poultry red mite (<i>Dermanyssus gallinae</i>) (2021)
  • Author(s): Ariizuim, T., Murata, S., Fujisawa, S., Isezaki, M., Maekawa, N., Okagawa, T., Sato, T., Oishi, E., Taneno, A., Konnai, S., Ohashi, K.
  • Journal Title: Journal of Veterinary Medical Science Volume: 83 Issue: 4 Pages: 558-565
  • DOI: 10.1292/jvms.20-0677
  • NAID: 130008023262
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens (2021)
  • Author(s): Murata, S., Taniguchi, A., Isezaki, M., Fujisawa, S., Sakai, E., Taneno, A., Ichii, O., Ito, T., Maekawa, N., Okagawa, T., Konnai, S., Ohashi, K.
  • Journal Title: Parasite Volume: 28 Pages: 9-9
  • DOI: 10.1051/parasite/2021005
  • Peer Reviewed / Open Access
• [Journal Article] PD-L1 immunohistochemistry for canine cancers and clinical benefit of anti-PD-L1 antibody in dogs with pulmonary metastatic oral malignant melanoma (2021)
  • Author(s): Maekawa, N., Konnai, S., Nishimura, M., Kagawa, Y., Takagi, S., Hosoya, K., Ohta, H., Kim, S., Okagawa, T., Izumi, Y., Deguchi, T., Kato, Y., Yamamoto, S., Yamamoto, K., Toda, M., Nakajima, C., Suzuki, Y., Murata, S., Ohashi, K.
  • Journal Title: npj Precision Oncology Volume: 5 Issue: 1 Pages: 10-10
  • DOI: 10.1038/s41698-021-00147-6
  • Peer Reviewed / Open Access
• [Journal Article] The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle infected with <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (2021)
  • Author(s): Sajiki, Y., Konnai, S., Nagata, R., Kawaji, S., Nakamura, H., Fujisawa, S., Okagawa, T., Mawkawa, N., Kato, Y., Suzuki, Y., Murata, S., Mori, Y., Ohashi, K.
  • Journal Title: Journal of Veterinary Medical Science Volume: 83 Issue: 2 Pages: 162-166
  • DOI: 10.1292/jvms.20-0590
  • NAID: 130007990713
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Direct evidence of the preventive effect of milk replacer?based probiotic feeding in calves against severe diarrhea (2021)
  • Author(s): Kayasaki, F., Okagawa, T., Konnai, S., Kohara, J., Sajiki, Y., Watari, K., Ganbaatar, O., Goto, S., Nakamura, H., Shimakura, H., Minato, E., Kobayashi, A., Kubota, M., Terasaki, N., Takeda, A., Noda, H., Honma, M., Maekawa, N., Murata, S., Ohashi, K.
  • Journal Title: Veterinary Microbiology Volume: 254 Pages: 108976-108976
  • DOI: 10.1016/j.vetmic.2020.108976
  • Peer Reviewed / Open Access
• [Journal Article] Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression (2021)
  • Author(s): Sajiki, Y., Konnai, S., Ikenaka, Y., Gulay, K. C. M., Kobayashi, A. Parizi, L. F., Joo, B. C., Watari, K., Fujisawa, S., Okagawa, T., Maekawa, N., Logullo, C.,da Silva Vaz, I.,Murata, S., Ohashi, K.
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 1063-1063
  • DOI: 10.1038/s41598-020-80251-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A TLR7 agonist activates bovine Th1 response and exerts antiviral activity against bovine leukemia virus (2021)
  • Author(s): Sajiki, Y., Konnai, S., Okagawa, T., Maekawa, N., Nakamura, H., Kato, Y., Suzuki, Y., Murata, S., Ohashi, K.
  • Journal Title: Developmental & Comparative Immunology Volume: 114 Pages: 103847-103847
  • DOI: 10.1016/j.dci.2020.103847
  • Peer Reviewed / Open Access
• [Journal Article] Molecular detection and genetic characterization of infectious laryngotracheitis virus in poultry in Myanmar (2020)
  • Author(s): Yang, Z., Murata, S., Fujisawa, S., Takehara, M., Katakura, Ken., Hmoon, M. M., Win, S. Y., Bawm, S., Konnai, S., Ohashi, K.
  • Journal Title: BMC Veterinary Research Volume: 16 Issue: 1 Pages: 453-453
  • DOI: 10.1186/s12917-020-02666-z
  • Peer Reviewed / Open Access
• [Journal Article] Distinct polymorphisms in a single herpesvirus gene are capable of enhancing virulence and mediating vaccinal resistance (2020)
  • Author(s): Conradie, A. M., Bertzbach, L. D., Trimpert, J., Patria, J. N., Murata, S., Parcells M. S.,Kaufer, B. B.
  • Journal Title: PLOS Pathogens Volume: 16 Issue: 12 Pages: e1009104-e1009104
  • DOI: 10.1371/journal.ppat.1009104
  • Peer Reviewed / Open Access
• [Journal Article] Genetic characterization of a Marek’s disease virus strain isolated in Japan (2020)
  • Author(s): Murata, S., Machida, Y., Isezaki, M., Maekawa, N., Okagawa, T., Konnai, S., Ohashi, K.
  • Journal Title: Virology Journal Volume: 17 Issue: 1 Pages: 186-186
  • DOI: 10.1186/s12985-020-01456-1
  • Peer Reviewed / Open Access
• [Journal Article] Detection and molecular identification of Leucocytozoon and Plasmodium species from village chickens in different areas of Myanmar (2020)
  • Author(s): Win, S. Y., Chel, H. M., Hmoon, M. M., Htun, L. L., Bawm, S., Win, M. M., Murata, S., Nonaka, N., Nakao, R., Katakura, K.
  • Journal Title: Acta Tropica Volume: 212 Pages: 105719-105719
  • DOI: 10.1016/j.actatropica.2020.105719
  • Peer Reviewed
• [Journal Article] Transcriptome dynamics of blood-fed and starved poultry red mites, Dermanyssus gallinae (2020)
  • Author(s): Fujisawa, S., Murata, S., Isezaki, M., Oishi, E., Taneno, A., Maekawa, N., Okagawa, T., Konnai, S., Ohashi, K.
  • Journal Title: Parasitology International Volume: 78 Pages: 102156-102156
  • DOI: 10.1016/j.parint.2020.102156
  • NAID: 120007160301
  • Peer Reviewed
• [Journal Article] Enhanced Immunotherapeutic Efficacy of Anti?PD-L1 Antibody in Combination with an EP4 Antagonist (2020)
  • Author(s): Sajiki Yamato、Konnai Satoru、Cai Zimeng、Takada Kensuke、Okagawa Tomohiro、Maekawa Naoya、Fujisawa Sotaro、Kato Yukinari、Suzuki Yasuhiko、Murata Shiro、Ohashi Kazuhiko
  • Journal Title: ImmunoHorizons Volume: 4 Issue: 12 Pages: 837-850
  • DOI: 10.4049/immunohorizons.2000089
  • Peer Reviewed / Open Access
• [Journal Article] The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine Mycoplasmosis (2020)
  • Author(s): Sajiki Yamato、Konnai Satoru、Goto Shinya、Okagawa Tomohiro、Ohira Kosuke、Shimakura Honami、Maekawa Naoya、Gondaira Satoshi、Higuchi Hidetoshi、Tajima Motoshi、Hirano Yuki、Kohara Junko、Murata Shiro、Ohashi Kazuhiko
  • Journal Title: Frontiers in Veterinary Science Volume: 7 Pages: 2564-2569
  • DOI: 10.3389/fvets.2020.609443
  • NAID: 120007193553
  • Peer Reviewed / Open Access
• [Journal Article] PD-L1 expression in equine malignant melanoma and functional effects of PD-L1 blockade (2020)
  • Author(s): Ganbaatar, O., Konnai, S. Okagawa, T., Nojima, Y., Maekawa, N., Minato, E., Kobayashi, A., Ando, R., Sasaki, N., Miyakoshi, D., Ichii, O., Kato, Y., Suzuki, Y., Murata, S., Ohashi, K.
  • Journal Title: PLOS ONE Volume: 15 Issue: 11 Pages: e0234218-e0234218
  • DOI: 10.1371/journal.pone.0234218
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification and functional analysis of ferritin 2 from the Taiga tick Ixodes persulcatus Schulze (2020)
  • Author(s): Githaka, N. W., Konnai, S., Isezaki, M., Goto, S., Xavier, M. A., Fujisawa, S., Yamada, S., Okagawa, T., Maekawa, N., Logullo, C., da Silva Vaz, I., Murata, S., Ohashi, K. 4.巻 11
  • Journal Title: Ticks and Tick-borne Diseases Volume: 11 Issue: 6 Pages: 101547-101547
  • DOI: 10.1016/j.ttbdis.2020.101547
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Expression Analysis of Canine CMTM6 and CMTM4 as Potential Regulators of the PD-L1 Protein in Canine Cancers (2020)
  • Author(s): Takeuchi, H., Konnai, S., Maekawa, N., Minato, E., Ichikawa, Y., Kobayashi, A., Okagawa, T., Murata, S., Ohashi, K.
  • Journal Title: Frontiers in Veterinary Science Volume: 7 Pages: 330-330
  • DOI: 10.3389/fvets.2020.00330
  • Peer Reviewed / Open Access
• [Journal Article] Upregulation of PD-L1 Expression by Prostaglandin E2 and the Enhancement of IFN-γ by Anti-PD-L1 Antibody Combined With a COX-2 Inhibitor in Mycoplasma bovis Infection (2020)
  • Author(s): Goto Shinya、Konnai Satoru、Hirano Yuki、Kohara Junko、Okagawa Tomohiro、Maekawa Naoya、Sajiki Yamato、Watari Kei、Minato Erina、Kobayashi Atsuhi、et al.
  • Journal Title: Frontiers in Veterinary Science Volume: 7 Pages: 12-12
  • DOI: 10.3389/fvets.2020.00012
  • Peer Reviewed / Open Access
• [Journal Article] Immunosuppressive Effects of Sialostatins L and L2 Isolated from the Taiga Tick Ixodes persulcatus Schulze (2020)
  • Author(s): Sajiki Y, Konnai S, Ochi A, Okagawa T, Githaka N, Isezaki M, Yamada S, Ito T, Ando S, Kawabata H, Da Silva Vaz Jr I, Logullo C, Maekawa N, Murata S, Ohashi K.
  • Journal Title: Ticks Tick born Dis. Volume: 11(2) Issue: 2 Pages: 101332-101332
  • DOI: 10.1016/j.ttbdis.2019.101332
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Haematophagous mites on poultry farms in the Republic of the Union of Myanmar (2019)
  • Author(s): Takehara M, Murata S, Katakura K, Fujisawa S, Hmoon MM, Win SY, Bawm S, Htun LL, Aung YH, Win MM, Isezaki M, Maekawa N, Okagawa T, Konnai S, Ohashi K.
  • Journal Title: Heliyon Volume: 5 Issue: 4 Pages: e01544-e01544
  • DOI: 10.1016/j.heliyon.2019.e01544
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular detection and genetic characterization of Mycoplasma gallisepticum, Mycoplama synoviae, and infectious bronchitis virus in poultry in Myanmar (2019)
  • Author(s): Fujisawa S, Murata S, Takehara M, Katakura K, Hmoon MM, Win SY, Ohashi K.
  • Journal Title: BMC Veterinary Research Volume: 15 Issue: 1 Pages: 261-261
  • DOI: 10.1186/s12917-019-2018-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prostaglandin E2 Induced Immune Exhaustion and Enhancement of Antiviral Effects by Anti-PD-L1 Antibody Combined with COX-2 Inhibitor in Bovine Leukemia Virus Infection (2019)
  • Author(s): Sajiki Yamato、Konnai Satoru、Okagawa Tomohiro、Nishimori Asami、Maekawa Naoya、Goto Shinya、Watari Kei、Minato Erina、Kobayashi Atsushi、et al.
  • Journal Title: The Journal of Immunology Volume: 203 Issue: 5 Pages: 1313-1324
  • DOI: 10.4049/jimmunol.1900342
  • Peer Reviewed / Open Access
• [Journal Article] Immune inhibitory function of bovine CTLA-4 and the effects of its blockade in IFN-γ production (2019)
  • Author(s): Watari K, Konnai S, Maekawa N, Okagawa T, Suzuki Y, Murata S, Ohashi K.
  • Journal Title: BMC Vet Res. Volume: 15(1) Issue: 1 Pages: 380-380
  • DOI: 10.1186/s12917-019-2082-7
  • Peer Reviewed / Open Access
• [Journal Article] Effects of bovine tumor necrosis factor alpha decoy receptors on cell death and inflammatory cytokine kinetics: potential for bovine inflammation therapy (2019)
  • Author(s): Fujisawa Sotaro、Konnai Satoru、Okagawa Tomohiro、Maekawa Naoya、Tanaka Akina、Suzuki Yasuhiko、Murata Shiro、Ohashi Kazuhiko
  • Journal Title: BMC Veterinary Research Volume: 15 Issue: 1 Pages: 68-68
  • DOI: 10.1186/s12917-019-1813-0
  • Peer Reviewed / Open Access
• [Journal Article] Cooperation of PD-1 and LAG-3 in the exhaustion of CD4+ and CD8+ T cells during bovine leukemia virus infection (2018)
  • Author(s): Okagawa Tomohiro、Konnai Satoru、Nishimori Asami、Maekawa Naoya、Goto Shinya、Ikebuchi Ryoyo、Kohara Junko、Suzuki Yasuhiko、Yamada Shinji、Kato Yukinari、Murata Shiro、Ohashi Kazuhiko
  • Journal Title: Veterinary Research Volume: 49 Issue: 1 Pages: 50-50
  • DOI: 10.1186/s13567-018-0543-9
  • Peer Reviewed / Open Access
• [Journal Article] Identification of immuno-inhibitory molecules in Mongolian native cattle and yak (2018)
  • Author(s): Ochirkhuu Nyamusuren、Konnai Satoru、Odbileg Raadan、Okagawa Tomohiro、Murata Shiro、Ohashi Kazuhiko
  • Journal Title: Jouranl of Japanese Veterinary Research Volume: 66 Pages: 177-192
  • NAID: 120006498199
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Prostaglandin E2 induction suppresses the Th1 immune responses in cattle with Johne's disease. (2018)
  • Author(s): Sajiki Y, Konnai S, Okagawa T, Nishimori A, Maekawa N, Goto S, Ikebuchi R, Nagata R, Kawaji S, Kagawa Y, Yamada S, Kato Y, Nakajima C, Suzuki Y, Murata S, Mori Y, Ohashi K.
  • Journal Title: Infect. Immun. Volume: in press Issue: 5
  • DOI: 10.1128/iai.00910-17
  • Peer Reviewed
• [Presentation] 牛白血病ウイルス感染症における免疫抑制受容体TIM-3の発現解析および機能解析 (2020)
  • Author(s): 中村 隼人, 今内 覚, 岡川 朋弘, 佐治木 大和, 渡 慧, 神谷 可菜, 齋藤 麻矢, 前川 直也, 村田 史郎, 大橋 和彦
  • Organizer: 第163回日本獣医学会学術集会
• [Presentation] ネコCytotoxic T-lymphocyte antigen-4 (CTLA-4) を標的とした新規免疫抑制製剤の開発における基礎的検討 (2020)
  • Author(s): 大塚 拓海, 今内 覚, 前川 直也, 渡 慧, 岡川 朋弘, 村田 史郎, 大橋 和彦
  • Organizer: 第163回日本獣医学会学術集会
• [Presentation] ワクモにおける定量的PCR法確立のための内在性コントロール遺伝子の探索 (2020)
  • Author(s): 有泉 拓馬, 村田 史郎, 藤澤 宗太郎, 伊勢崎 政美, 前川 直也, 岡川 朋弘, 種子野 章, 大石 英司, 今内 覚, 大橋 和彦
  • Organizer: 第163回日本獣医学会学術集会
• [Presentation] マレック病に関する最近の知見について ―日本に分布するマレック病ウイルスの性状 (2019)
  • Author(s): 村田 史郎、今内 覚、大橋 和彦
  • Organizer: 2019年度佐賀県支部第1回鶏病技術研修会
  • Invited
• [Presentation] ワクモ由来Adipocyte plasma membrane-associated proteinの抗ワクモワクチン抗原としての評価 (2019)
  • Author(s): 竹原 昌生、森田 鮎、村田 史郎、伊勢崎 政美、藤澤 宗太郎、種子野 章、酒井 英史、宇野 有紀子、小川 遼、市居 修、前川 直也、岡川 朋弘、今内 覚、大橋和彦
  • Organizer: 令和元年度 鶏病研究会北海道支部技術検討会
• [Presentation] Transcriptome analysis in blood-fed and starved poultry red mites, Dermanyssus gallinae (2019)
  • Author(s): Fujisawa, S., Murata, S.,, Takehara, M., Isezaki, M., Ogawa, R., Uno, Y., Taneno, A., Okagawa, T., Maekawa, N., Konnai, S., Ohashi, K.
  • Organizer: 4th International Symposium on Parasite Infection in Poultry
  • Int'l Joint Research
• [Presentation] Cysteine protease and ferritin 2 as vaccine antigens to control poultry red mites, Dermanyssus gallinae (2019)
  • Author(s): Murata, S., Isezaki, M., Taniguchi, A., Fujisawa, S., Taneno, A., Sakai, E., Uno, Y., Ogawa, R., Ichii, O., Ito, T., Takehara, M., Morita, A., Maekawa, N., Okagawa, T., Konnai, S., Ohashi, K.
  • Organizer: 4th International Symposium on Parasite Infection in Poultry
  • Int'l Joint Research
• [Presentation] Establishment of anti-bovine PD-1 chimeric antibody and a pilot clinical study (2019)
  • Author(s): Konnai, S., Okagawa, T., Maekawa, N., Murata, S., Ohashi, K.
  • Organizer: International Veterinary Immunology Symposium 2019
  • Int'l Joint Research
• [Presentation] Contribution of prostaglandin E2 to disease progression and enhancement of antiviral effects by anti-PD-L1 antibody combined with COX-2 inhibitor in bovine leukemia virus infection (2019)
  • Author(s): Sajiki, Y., Konnai, S., Okagawa, T., Maekawa, N., Murata, S., Ohashi, K.
  • Organizer: International Veterinary Immunology Symposium 2019
  • Int'l Joint Research
• [Presentation] Immunomodulatory effects of sialostatin L and sialostatin L2 from Ixodes persulcatus Schulze, Taiga tick (2019)
  • Author(s): Konnai, S., Okagawa, T., Maekawa, N., Murata, S., Ohashi, K.
  • Organizer: International Veterinary Immunology Symposium 2019
  • Int'l Joint Research
• [Presentation] The induction of immunosuppression via prostaglandin E2 and the enhancement of anti-bacterial effects by anti-PD-L1 antibody combined with COX-2 inhibitor in Mycoplasma bovis infection (2019)
  • Author(s): Goto, S., Konnai, S., Okagawa, T., Maekawa, N., Murata, S., Ohashi, K.
  • Organizer: International Veterinary Immunology Symposium 2019
  • Int'l Joint Research
• [Presentation] Functional analysis of bovine CTLA-4 in bovine leukemia virus infection (2019)
  • Author(s): Watari, K., Konnai, S., Okagawa, T., Maekawa, N., Murata, S., Ohashi, K.
  • Organizer: International Veterinary Immunology Symposium 2019
  • Int'l Joint Research
• [Presentation] Effects of bovine tumor necrosis factor alpha decoy receptors on cell death and inflammatory cytokine kinetics: potential for bovine inflammation therapy (2019)
  • Author(s): Fujisawa, S., Konnai, S., Okagawa, T., Maekawa, N., Murata, S., Ohashi, K.
  • Organizer: International Veterinary Immunology Symposium 2019
  • Int'l Joint Research
• [Presentation] Development of a sensitive PD-L1 immunohistochemistry of canine cancers and clinical efficacy of an anti-PD-L1 antibody in canine oral malignant melanoma with pulmonary metastasis (2019)
  • Author(s): Maekawa, N., Konnai, S., Okagawa, T., Murata, S., Ohashi, K.
  • Organizer: International Veterinary Immunology Symposium 2019
  • Int'l Joint Research
• [Presentation] ロタウイルス実験感染子牛モデルを用いた3.5倍発酵代用乳の腸炎抑制効果の検証 (2019)
  • Author(s): 茅先 史, 今内 覚, 久保田 学, 岡川朋弘, 佐治木大和, 渡 慧, 小原潤子, 前川直也, 村田史郎, 大橋 和彦
  • Organizer: 北海道獣医師会学術集会
• [Presentation] BLV感染症に対するCOX-2阻害剤と 抗PD-L1抗体併用法の抗ウイルス効果の検討 (2019)
  • Author(s): 佐治木 大和、今内 覚、岡川 朋弘、前川 直也、後藤 伸也、小原 潤子、 山田 慎二、加藤 幸成、鈴木 定彦、村田 史郎、大橋 和彦
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] Mycoplasma bovis感染症に対する抗PD-L1キメラ抗体を用いた臨床試験 (2019)
  • Author(s): 後藤 伸也、今内 覚、岡川 朋宏、前川 直也、小原 潤子、平野 佑気、鈴木 定彦、村田 史郎、大橋 和彦
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] COX-2阻害剤の併用による抗PD-L1抗体を用いたイヌ腫瘍免疫療法の効果増強に向けた基礎的検討 (2019)
  • Author(s): 前川 直也、今内 覚、浅野 裕美恵、岡川 朋弘、高木 哲、村田 史郎、大橋 和彦
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] イヌ腫瘍由来細胞株におけるCKLF-like MARVEL transmembrane domain containing protein 6 (CMTM6) および4 (CMTM4) の発現解析 (2019)
  • Author(s): 竹内 寛人、今内 覚、岡川 朋弘、前川 直也、村田 史郎、大橋 和彦
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] イヌ腫瘍組織およびイヌ腫瘍由来細胞株におけるイヌHER2の発現解析 (2019)
  • Author(s): 吉武 志江奈、今内 覚、前川 直也、賀川 由美子、西村 麻紀、岡川 朋弘、鈴木 定彦、高木 哲、中川 貴之、村田 史郎、大橋 和彦
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] Molecular detection and genetic characterization of Mycoplasma gallisepticum, Mycoplama synoviae, infectious bronchitis virus and infectious laryngotracheitis virus in poultry farms in Myanmar (2019)
  • Author(s): Yang Zhiyuan、藤澤 宗太郎、村田 史郎、竹原 昌生、片倉 賢、Myint Myint Hmoon、Shwe Yee Win、Saw Bawm、Lat Lat Htun、Ye Htut Aung、Mar Mar Win、今内 覚、大橋 和彦
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] ワクモ由来Adipocyte plasma membrane-associated proteinの抗ワクモワクチン抗原としての評価 (2019)
  • Author(s): 森田 鮎、竹原 昌生、村田 史郎、伊勢崎 政美、藤澤 宗太郎、種子野 章、酒井 英史、宇野 有紀子、小川 遼、市居 修、前川 直也、岡川 朋弘、今内覚、大橋和彦
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] RNA-Seqを用いたワクモ（Dermanyssus gallinae）の吸血状態別における遺伝子発現解析 (2019)
  • Author(s): 藤澤 宗太郎、村田 史郎、竹原 昌生、伊勢崎 政美、小川 遼、宇野 有紀子、種子野 章、前川 直也、岡川 朋弘、今内 覚、大橋 和彦
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] Molecular detection of Marek's disease virus in poultry farms in Myanmar (2018)
  • Author(s): Shiro Murata、Masaki Takehara、Ken Katakura、Myint Myint Hmoon、Shwe Yee Win、Masayoshi Isezaki、Satoru Konnai、Kazuhiko Ohashi
  • Organizer: The 12th International Symposium on Marek’s disease and Avian Herpesviruses
  • Int'l Joint Research
• [Presentation] ミャンマー連邦共和国における鶏の吸血性外部寄生虫の分布状況調査 (2018)
  • Author(s): 竹原昌生、村田史郎、片倉賢、Myint Myint Hmoon、Shwe Yee Win、Saw Bawm、Lat Lat Htun、Ye Htut Aung、Mar Mar Win、伊勢崎政美、今内覚、大橋和彦
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] 動物難治性疾病の免疫学的解析を基盤とした新規制御法の開発 (2018)
  • Author(s): 今内覚、村田史郎、大橋和彦
  • Organizer: 第161回日本獣医学会学術集会
  • Invited
• [Presentation] マレック病ウイルスの病原性新海機構の解明ー病原性試験や全ゲノム解析の試み (2018)
  • Author(s): 大橋和彦、村田史郎、町田柚香、伊勢崎政美、今内覚
  • Organizer: 第161回日本獣医学会学術集会
  • Invited
• [Presentation] 免疫抑制因子PD-1およびPD-L1を標的としたイヌ腫瘍に対する新規免疫療法の検討 (2018)
  • Author(s): 前川直也、今内覚、村田史郎、大橋和彦
  • Organizer: 第161回日本獣医学会学術集会
  • Invited
• [Presentation] ウシCytotoxic T-lymphocyte antigen-4 (CTLA-4)の機能解析および慢性感染症における発現解析 (2018)
  • Author(s): 渡慧、今内覚、岡川朋弘、前川直也、後藤伸也、佐治木大和、村田史郎、鈴木定彦、大橋和彦
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] ヨーネ病におけるプロスタグランジンE2（PGE2）動態解析及びCOX-2阻害剤の免疫活性効果の検討 (2018)
  • Author(s): 佐治木大和、今内覚、岡川朋弘、西森朝美、前川直也、後藤伸也、永田礼子、川治聡子、森康行、村田史郎、大橋和彦
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] Mycoplasma bovis感染症における免疫抑制機序の解析 (2018)
  • Author(s): 後藤伸也、今内覚、岡川朋弘、前川直也、佐治木大和、渡慧、樋口豪起、小岩政照、田島誉士、鈴木定彦、村田史郎、大橋和彦
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] 地方病性牛白血病若齢発症牛におけるプロウイルス挿入部位の網羅的解析 (2018)
  • Author(s): 岡川朋弘、今内覚、西森朝美、田中晶菜、前川直也、戸塚知恵、千葉由純、池田昌穂、村田史郎、大橋和彦
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] 地方病性牛白血病若齢発症牛の簡易鑑別診断基準の確立と若齢発症牛の実態調査 (2018)
  • Author(s): 田中晶菜、今内覚、岡川朋弘、西森朝美、前川直也、戸塚知恵、千葉由純、池田昌穂、村田史郎、大橋和彦
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] 肺転移のある悪性黒色腫罹患犬に対するPD-L1を標的とした抗体薬の治療効果 (2018)
  • Author(s): 前川直也、今内覚、高木哲、細谷謙次、賀川由美子、岡川朋弘、和泉雄介、出口辰弥、鈴木定彦、山本啓一、村田史郎、大橋和彦
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] イヌCytotoxic T-lymphocyte antigen-4 (CTLA-4)を標的としたバイオ医薬品の開発に向けた基礎的検討 (2018)
  • Author(s): 石原悠太郎、今内覚、岡川朋弘、前川直也、鈴木定彦、大田寛、村田史郎、大橋和彦
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] 抗ワクモワクチン開発に向けた試み (2018)
  • Author(s): 村田史郎
  • Organizer: 第２８４回鶏病事例検討会
  • Invited
• [Presentation] ワクモ（Dermanyssus gallinae）由来カテプシンL様タンパク質およびフェリチン2を用いた抗ワクモワクチンの開発 (2018)
  • Author(s): 村田史郎、宇野有紀子、酒井英史、種子野章、伊勢崎政美、谷口綾香、竹原昌生、今内覚、大橋和彦
  • Organizer: 平成30年度慧病研究会北海道支部技術検討会
• [Remarks] 北海道大学大学院獣医学研究院 感染症学教室ホームページ
  • URL: https://lab-inf.vetmed.hokudai.ac.jp
• [Remarks] 北海道大学大学院獣医学研究院 感染症学教室ホームページ
  • URL: https://lab-inf.vetmed.hokudai.ac.jp