| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
The pathogenesis of Neospora-induced abnormal births was analyzed to prevent abortions in domestic animals due to infection with Neospora caninum. A mouse model of abnormal birth due to infection was established and the inflammatory response in utero-placental tissue was observed. We constructed a cell screening system based on the signal transduction of inflammatory response and found NcGRA7 as a protozoan-derived factor inducing abnormal birth. NcGRA7-deficient strain was generated and their virulence was tested in non-pregnant and pregnant mouse models, showing that NcGRA7 deficiency reduced the parasite virulence and increased normal pregnancy rates. Host protein X, which binds to NcGRA7, was also identified by mass spectrometry. Expression of NcGRA7 was also observed in tissue samples from bovine neosporosis, suggesting that NcGAR7 will be involved in Neospora abortion.
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