| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Outline of Final Research Achievements |
In this project, we made two lines of knock-in mice that express meiosis-specific cohesin subunit, RAD21L or REC8, tagged with 3×FLAG. We determined the expression ratio of RAD21L and REC8 and the absolute amount of cohesins in a mouse spermatocyte by western blot analysis with anti-3×FLAG antibody. Furthermore, we sought to identify RAD21L- and REC8-associated proteins by MS/MS analysis following immunoprecipitation using testes extracts from wild-type (control) and knock-in mice. As a result, besides the proteins that are known to associate with mitotic cohesin, a novel protein whose interaction with cohesin has never known was found in the immunoprecipitates.
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