| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Outline of Final Research Achievements |
In this study, we established a method for observing nuclear chromatin dynamics during follicle development and maturation, and evaluated it by live-cell imaging. In particular, as a result of observing the nuclear localization of the pericentromeric region existing near the chromosomal centromere, we succeeded in continuously evaluating the dynamics of methylated DNA in the nucleus in three dimensions. We also revealed that nuclear chromatin dynamics during follicle maturation are associated with development of embryos. Moreover, we succeeded in evaluating the nuclear chromatin dynamics during follicle development and oocyte maturation under minimally invasive conditions and linking them to the subsequent development. The methodology established in the present study provided a new methodology for the study of oogenesis.
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