Elucidation of the molecular mechanism to maintain hierarchical structure of vascular system and and tumour angiogenesis

Research Project

Project/Area Number	18H02363
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 42040:Laboratory animal science-related
Research Institution	Shiga University of Medical Science
Principal Investigator	Ema Masatsugu 滋賀医科大学, 動物生命科学研究センター, 教授 (60359578)
Co-Investigator(Kenkyū-buntansha)	水野聖哉筑波大学, 医学医療系, 准教授 (10633141) 杉山文博筑波大学, 医学医療系, 教授 (90226481)
Project Period (FY)	2018-04-01 – 2021-03-31
Project Status	Completed (Fiscal Year 2020)
Budget Amount *help	¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000) Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords	血管新生 / 血管内皮細胞 / マウス / 遺伝子改変 / リンパ管新生 / エキソサイトーシス / 腫瘍血管新生 / イメージング / ノックアウトマウス
Outline of Final Research Achievements	We have identified Exoc3-like family genes which are specifically expressed in developing endothelial cells. To investigate the physiological roles of Exoc3L genes, we have created Exoc3L2-GFP knock-in mouse and found that GFP (Exoc3L2) is expressed in endothelial cells and KO embryos die in utero due to breeding. We also created Exoc3L4-GFP knock-in mouse and found GFP (Exoc3L4) is expressed in endothelial cells as well as cardiomyocytes. Exoc3L4 KO embryos die around E12.5, due to unknown mechanism. We also created VEGFR3-Venus BAC Tg mouse useful for visualization of vascular and lymphatic endothelial cells.
Academic Significance and Societal Importance of the Research Achievements	Exoc3L遺伝子群の生理機能を体系的に明らかにし、その制御機構を解明することができれば、新たな腫瘍血管新生抑制療法の開発に繋がるものと期待される。