Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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Outline of Final Research Achievements |
We have identified Exoc3-like family genes which are specifically expressed in developing endothelial cells. To investigate the physiological roles of Exoc3L genes, we have created Exoc3L2-GFP knock-in mouse and found that GFP (Exoc3L2) is expressed in endothelial cells and KO embryos die in utero due to breeding. We also created Exoc3L4-GFP knock-in mouse and found GFP (Exoc3L4) is expressed in endothelial cells as well as cardiomyocytes. Exoc3L4 KO embryos die around E12.5, due to unknown mechanism. We also created VEGFR3-Venus BAC Tg mouse useful for visualization of vascular and lymphatic endothelial cells.
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