| Project/Area Number |
18H02364
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 42040:Laboratory animal science-related
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| Research Institution | Kyoto University (2019-2020)
Kobe University (2018) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
谷口 幸雄 京都大学, 農学研究科, 准教授 (10252496)
波多野 直哉 神戸大学, 医学研究科, 医学研究員 (10332280)
須山 幹太 九州大学, 生体防御医学研究所, 教授 (70452365)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | 糖尿病 / 疾患モデル / 遺伝素因 / 病態発症機構 / オミクス / 膵島障害 |
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| Outline of Final Research Achievements |
In this study, by using the Zucker fatty diabetes mellitus (ZFDM) rat, a novel rat model of type 2 diabetes which has been developed by the representative researcher’s group, we performed genetic analysis and exome analysis to identify diabetes susceptibility genes, CRISPR/Cas9-mediated genome editing of the candidate gene to verify the causality, mRNA-sequencing and metabolome analysis to elucidate longitudinal changes in metabolic dysfunction, and proteome analysis to identify abnormally modified proteins, resulting in the clarification of a part of genetic factors and mechanisms involved in the development and progression of the disease.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により明らかとなったZFDMラットにおける遺伝素因と膵島障害の発症・進展機構は、ヒト糖尿病の遺伝素因と病態発症・進展機構の解明や新規のメカニズムに基づく治療薬の開発の基盤となると考えられる。また、同様の研究手法が適用可能な様々の研究分野において疾患モデルの有用性を実証するものである。
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