| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2022: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Outline of Final Research Achievements |
This study aimed to clarify the nature of cleaved histone H3, which is abundantly observed in testicular germ cells, and its transgenerational effects. We found that at least two types of cleaved histone H3s exist in the germ cell differentiation-dependent manner, and one of them was cleaved at the Ala21-Thr22 of the N-terminal tail (designated as csH3). The csH3 was cleaved by cathepsin L (CTSL) in the testis, and it accumulated in the XY body of the spermatocytes, which occurred downstream of the DNA damage response. To test its transgenerational effect by ICSI, we attempted to induce differentiation of spermatocytes without csH3 to spermatids but failed to obtain spermatids due to the impaired differentiation. This study has provided insight into the presence and ontogeny of cleaved histones in spermatogenesis, their subcellular localization, cleaving enzymes, and signaling pathways upstream of cleavage.
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