| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
In this study, we aimed to understand the mechanisms of how sister chromatid cohesion in heterochromatin region is established. Dalmatian (Dmt) is known as a cohesion establishment factor in Drosophila melanogaster. Through non-biased screening of Dmt-binding proteins, we identified 25 candidate genes, of which knockdown exhibited abnormal cohesion. Among them, we found that BAP180, a subunit of PBAP chromatin remodeling complex, is essential role in cohesion. In BAP180-depleted cells, Dmt couldn’t be localized to heterochromatin, however cohesin itself normally associated with chromatin. Overexpression of Dmt restored cohesion in BAP180-depleted cells. These results suggest that BAP180 has a crucial role in cohesion establishment in heterochromatin by regulating Dmt localization.
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