| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Outline of Final Research Achievements |
We achieved the aims of our project by identifying new, important mechanisms in the pathway leading from asymmetric crossover placement to the establishment of cohesion loss strictly on one of two domains on chromosomes of the nematode C. elegans. We showed that the synaptonemal complex central element protein SYP-1 and the HORMA domain protein HIM-3 both become phosphorylated specifically on the shorter of two domains created by the asymmetric placement of a single crossover, and that preventing this phosphorylation leads to failure of downstream recruitment of factors that promote chromosome segregation.
Additionally, we showed that the partitioning of phosphoproteins to the short arm depends globally on the number of crossovers in the nucleus, with partitioning failing when fewer than 4 crossovers are present. This result indicates global feedback mechanisms work to promote asymmetry of chromosome protein recruitment.
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| Academic Significance and Societal Importance of the Research Achievements |
Since chromosome segregation in meiosis is critical for fertility and correct development, our work leads to further insight about the possible causes of failure in human meiosis, which is responsible for a significant portion of both infertility and developmental atypicalities in humans.
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