| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2018: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
A fundamental of life is to coordinate the chromosome architecture with cell cycle progression in order to faithfully propagate genetic information. In this study, we aimed at addressing the molecular and cellular mechanisms controlling cell division in coordination with chromosome replication termination. We identified ZapT as a novel cell division protein associated with DNA. ZapT is required for normal cell division, ensuring strict colocalization of divisome with chromosome terminus. We show that ZapT has the affinity for both divisome and chromosome terminus, which help to organize the terminus DNA into a clustered structure over the divisome. Biochemical characterization revealed that ZapT and the divisome subunits ZauP and ZapA interacted directly to form a highly ordered ternary complex. Together, we propose that ZauP-dependent clustering of ZapT-DNA complexes plays a distinct role in organizing the replication terminus and the Z-ring.
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