| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Outline of Final Research Achievements |
We synthesized new ganglioside probes which partitioned into raft fraction. Single-fluorescent molecule imaging of these new ganglioside probes revealed that 1) all the observed gangliosides formed transient homodimers, 2) the ganglioside homodimers were formed by the specific glycan-glycan interactions, 3) the ganglioside homodimers transiently recruited other raftophilic molecules, and formed tiny rafts. Furthermore, 4) the homodimer formation of gangliosides by specific glycan-glycan interactions was supported by the all-atom molecular dynamics simulations, and we revealed that 5) dimerization and activation of EGF receptors are inhibited by the specific glycan-glycan interactions of GM3 homodimers with N-glycans of EGF receptors.
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