| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
Intramembrane cleaving proteases (I-CLiPs) are known to act in a variety of important cellular processes in diverse organisms. RseP, an Escherichia coli S2P family intramembrane cleaving protease, is involved in the regulation of the sigma E pathway extracytoplasmic stress response and membrane quality control through specific cleavage of substrates within the lipid bilayer. We have identified novel substrates of RseP and analyzed the physiological significance of their RseP-dependent cleavage. Also, we tried to construct an in vitro assay system that enables quantitative analysis of the RseP activity. These results would contribute to the elucidation of the cellular roles of and the mechanism underlying proteolysis by RseP.
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