Structural basis and mechanism of Sec holo translocon complex
| Project/Area Number |
18H02405
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 43030:Functional biochemistry-related
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
Tsukazaki Tomoya 奈良先端科学技術大学院大学, 先端科学技術研究科, 教授 (80436716)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Keywords | タンパク質の膜透過 / 構造生物学 / 膜蛋白質 / タンパク質 / トランスロコン / protein translocation |
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| Outline of Final Research Achievements |
To unveil the essential biological processes of living organisms, it is crucial to elucidate these molecular mechanisms. Protein translocation across the membrane is a fundamental mechanism in all living cells. In a model organism, Escherichia coli, a supramolecular protein complex including the membrane protein complex SecYEG is involved in the protein translocation. In this study, we tried to elucidate the structure and function of the supramolecular protein complex by X-ray crystallography, cryo-electron microscopy, and atomic force microscopy.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、必須の生命現象を分子レベル、原子レベルで明らかとしようとしている。有機化合物である分子がいかに生命活動を行っていくのかについては、いまだほとんど解明されていない状況である。学術的にこれらを明らかとしていくためには一つ一つの生命現象を解き明かす必要があり、本研究を達成することで、タンパク質の膜透過という一つの生命現象の詳細な理解につながる。そのため、本研究は学術的に極めて意義深いものである。
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Structural basis of sarco/endoplasmic reticulum Ca2+-ATPase 2b regulation via transmembrane helix interplay2019
Author(s)
Michio Inoue, Nanami Sakuta, Satoshi Watanabe, Yuxia Zhang, Kunihito Yoshikaie, Yoshiki Tanaka, Ryo Ushioda, Yukinari Kato, Junichi Takagi, Tomoya Tsukazaki, Kazuhiro Nagata, Kenji Inaba
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Journal Title
Cell Reports
Volume: 27
Issue: 4
Pages: 1221-1230
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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