Engineering of translation molecules for synthetic biology approach on the genetic code
| Project/Area Number |
18H02416
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 43040:Biophysics-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Sakamoto Kensaku 国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (50240685)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Keywords | 拡張遺伝暗号 / 非天然アミノ酸 / tRNA / アミノアシルtRNA合成酵素 / タンパク質合成 / 抗体 / 抗原親和性 / Fab / メチルグルタミン / 酵素変異体 / 合成生物学 / タンパク質工学 / 翻訳後修飾 / 遺伝暗号 / 非天然型アミノ酸 / 古細菌 / ピロリジン / アミノアシル化 / 立体構造 |
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| Outline of Final Research Achievements |
The field of ‘genetic code expansion’ or ‘genetic code engineering’ encompasses two directions: Technology developments for in vivo incorporation of non-natural amino acids into proteins and applications of this technology in medical and industrial spheres. The present study successfully addressed both aspects of the field. A new approach for creating ‘identityless’ tRNAs, which are of fundamental importance for expanding the code, was thus established. Another achievement was a novel method to improve the antigen-binding of antibodies, which illuminated a new direction of protein engineering based on expanded codes.
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| Academic Significance and Societal Importance of the Research Achievements |
遺伝暗号は全ての生き物にとっての“OS（オペレーティングシステム）”と言っても過言ではなく、拡張遺伝暗号とはその遺伝暗号がバージョンアップされたものである。合成生物学とは、生体分子、微生物等における有用な新規機能を将来に渉って持続的に開発することを可能にする学問であり、本課題では、非天然アミノ酸をタンパク質に導入するための遺伝暗号技術を一層発展させるとともに、社会的ニーズに答えるための応用研究を推進した。
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Report
(5 results)
Research Products
(17 results)
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[Journal Article] An integrated computational pipeline for designing high-affinity nanobodies with expanded genetic codes2021
Author(s)
Aditya K Padhi, Ashutosh Kumar, Ken-ichi Haruna, Haruna Sato, Hiroko Tamura, Satoru Nagatoishi, Kouhei Tsumoto, Atushi Yamaguchi, Fumie Iraha, Mihoko Takahashi, Kensaku Sakamoto, Kam Y J Zhang
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Journal Title
Briefings in Bioinformatics
Volume: 22
Issue: 6
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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