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Creating a biomolecular motor that moves on artificial tracks

Research Project

Project/Area Number 18H02417
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 43040:Biophysics-related
Research InstitutionNational Institute of Information and Communications Technology

Principal Investigator

Furuta Ken'ya  国立研究開発法人情報通信研究機構, 未来ICT研究所フロンティア創造総合研究室, 主任研究員 (40571831)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords分子モーター / DNAナノテクノロジー / DNA結合タンパク質 / モータータンパク質 / 合成生物学
Outline of Final Research Achievements

We have developed a chimeric molecule in which the microtubule-binding site of dynein is replaced with the DNA-binding site of a transcription factor to create a new protein-based molecular motor that moves on DNA. DNA nanotubes were prepared as tracks for this new molecular motor. The recognition sequences of DNA binding sites were incorporated into the surface of the DNA nanotubes. As a result, the DNA nanotubes showed a smooth sliding movement with an average speed of 220 nm/s. Moreover, we succeeded in creating a dimeric motor that moves processively on DNA nanotubes.

Academic Significance and Societal Importance of the Research Achievements

近年発達してきたナノテクノロジーでは自律的な小さな機械を数多く集めて使うことで複雑な仕事をさせるというアイディアに基づいて活発な研究が進められているが、これまで人工の分子マシンと、天然の生物分子モーターにはそれぞれに短所があり、どちらも応用には遠いと考えられていた。本研究で、天然の分子モーターと遜色のない速度を持ち、制御性の高いDNAナノ構造体上を動く実用的なモーターが得られたので、今後、DNAナノ構造体を高速で動かしたり、分子を自律的に輸送し濃縮したり計算したりするような新しいデバイスの構築に使用できる可能性があると考えている。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (7 results)

All 2020 2019 2018

All Presentation (7 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results)

  • [Presentation] タンパク質モータを創る2020

    • Author(s)
      古田 健也
    • Organizer
      未来ICTシンポジウム2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] Cytoplasmic dynein stepping on crowded microtubules resolved using dark-field imaging with high spatio-temporal resolution2019

    • Author(s)
      熊谷 優佑、柴田 桂太朗、古田 健也、本田 元、小嶋 寛明
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Collective motility of Chlamydomonas outer arm dynein measured using its intermediate chain as a scaffold for motility assays2019

    • Author(s)
      松田 祐佳、古田 茜、大岩 和弘、小嶋 寛明、古田 健也
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Measuring mechanical responses of Chlamydomonas axonemal dynein arrays to external load2019

    • Author(s)
      佐川 美咲、古田 茜、小嶋 寛明、大岩 和弘、古田 健也
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Re-design of linear molecular motors2019

    • Author(s)
      指宿 良太、古田 茜、森下 達矢、大岩 和弘、小嶋 寛明、古田 健也
    • Organizer
      第63回米国生物物理学会年会Saturday Subgroup Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生物分子モーターを再設計する2018

    • Author(s)
      古田健也
    • Organizer
      第15回AMO討論会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Re-design of linear molecular motors2018

    • Author(s)
      古田健也
    • Organizer
      仙台ワークショップ"An Update on Molecular Motors:Open Challenges and New Perspectives"
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2018-04-23   Modified: 2022-01-27  

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