Development of single-molecule measurement with angstrom precision
| Project/Area Number |
18H02418
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 43040:Biophysics-related
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| Research Institution | Institute for Molecular Science |
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Principal Investigator |
IINO Ryota 分子科学研究所, 生命・錯体分子科学研究領域, 教授 (70403003)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | 分子モーター / １分子計測 / 光学顕微鏡 / １分子イメージング |
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| Outline of Final Research Achievements |
We have developed an optical microscopic single-molecule method based on the scattering imaging of plasmonic metal nanoparticles, and achieved atomic-level (angstrom-level) localization precision and microsecond time resolution. In addition, the developed method has been successfully applied to linear and rotary molecular motors such as kinesin and V1-ATPase to elucidate their chemo-mechanical coupling mechanism and elementary process of motion in detail. Furthermore, we have developed a dual-color, high-speed/high-precision single-molecule measurement method using silver and gold nanoparticles as probes, and a method estimating chemical state-dependent free energy profile from the single-molecule trajectory of the movement of molecular motor.
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| Academic Significance and Societal Importance of the Research Achievements |
生体１分子オングストローム計測法により、高度な機能を発現する生体分子機械の作動原理を原子レベルの位置決定精度で解明することが可能になり、生体分子モーターのエネルギー変換機構の多様性や普遍性が明らかとなりつつある。また本手法は、生体分子機械よりもずっと小さな人工分子機械の原子レベルの動きの可視化にも有用と考えられ、生物学のみならず化学分野における応用展開も期待される。さらに、デュアルカラー高速高精度１分子計測法は３色以上のマルチカラーに発展させることが可能であり、従来の蛍光イメージング代わる汎用的バイオイメージング技術となり得る可能性が期待される。
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Report
(4 results)
Research Products
(17 results)
