| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
We have developed an optical microscopic single-molecule method based on the scattering imaging of plasmonic metal nanoparticles, and achieved atomic-level (angstrom-level) localization precision and microsecond time resolution. In addition, the developed method has been successfully applied to linear and rotary molecular motors such as kinesin and V1-ATPase to elucidate their chemo-mechanical coupling mechanism and elementary process of motion in detail. Furthermore, we have developed a dual-color, high-speed/high-precision single-molecule measurement method using silver and gold nanoparticles as probes, and a method estimating chemical state-dependent free energy profile from the single-molecule trajectory of the movement of molecular motor.
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