Funtional analysis of hormone-like peptides encoded by novel small coding genes

• Project/Area Number: 18H02420
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 43050:Genome biology-related
• Research Institution: Kyushu Institute of Technology
• Principal Investigator: Hanada Kousuke 九州工業大学, 大学院情報工学研究院, 教授 (50462718)
• Co-Investigator(Kenkyū-buntansha): 望田 啓子 (桑田啓子) 名古屋大学, トランスフォーマティブ生命分子研究所, 特任講師 (70624352) ; LEE JAEMAN 九州大学, 農学研究院, 准教授 (50404083)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2020: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: シロイヌナズナ / ペプチドホルモン / ペプチド / 短い遺伝子 / プロテオーム解析 / 植物 / ホルモン / 進化 / ホルモン様ペプチド

Outline of Final Research Achievements

Currently, the genomes are determined in many species but molecular function of small coding genes (<100aa) tend not to have any molecular functions. We inferred a large number of candidates of small coding genes in the intergenic region of Arabidopsis thaliana. Some of novel small coding genes show stress tolerance on overexpressed transgenic plants. To clarify the physilogical signals involved in such the genes, we try to examine molecular functions of novel small coding genes.

Academic Significance and Societal Importance of the Research Achievements

本研究の特色は、ペプチド性遺伝子にコードされるペプチドが、新しい生理活性物質として、農業に利用するための基盤研究にもなる。これらのペプチドを利用すると、遺伝子組み換え技術を利用しない、新しい農業革命につながる可能性があり、応用研究への意義も高い。また、本研究は、植物だけでなく、全ての生物のゲノムに同定されずにいるペプチド性遺伝子の重要性を把握することに繋がる。ペプチド性遺伝子が、ヒトや動物への新しい治療薬として役に立つ可能性もあり、基礎的な意味でも本研究を推進する意義は高い。

Research Products

• [Journal Article] Effect of small coding genes on the circadian rhythms under elevated CO2 conditions in plants (2020)
  • Author(s): Higuchi-Takeuchi Mieko、Kondo Takayuki、Shimizu Minami、Kim You-Wang、Shinozaki Kazuo、Hanada Kousuke
  • Journal Title: Plant Molecular Biology Volume: 104 Issue: 1-2 Pages: 55-65
  • DOI: 10.1007/s11103-020-01023-w
  • Peer Reviewed
• [Journal Article] Degree of Functional Divergence in Duplicates Is Associated with Distinct Roles in Plant Evolution (2020)
  • Author(s): Ezoe Akihiro、Shirai Kazumasa、Hanada Kousuke
  • Journal Title: Molecular Biology and Evolution Volume: 38 Issue: 4 Pages: 1447-1459
  • DOI: 10.1093/molbev/msaa302
  • Peer Reviewed / Open Access
• [Journal Article] Hormone-like peptides and small coding genes in plant stress signaling and development (2019)
  • Author(s): FuminoriTakahashi, KousukeHanada, TakayukiKondo, KazuoShinozaki
  • Journal Title: Current Opinion in Plant Biology Volume: 51 Pages: 88-95
  • DOI: 10.1016/j.pbi.2019.05.011
  • Peer Reviewed
• [Journal Article] Contribution of Functional Divergence through Copy Number Variations to the Inter-species and Intra-species Diversity in Specialized Metabolites (2019)
  • Author(s): Kazumasa Shirai, Kousuke Hanada
  • Journal Title: Frontiers in Plant Science Volume: 10 Pages: 1567-1567
  • DOI: 10.3389/fpls.2019.01567
  • Peer Reviewed / Open Access
• [Journal Article] Tuning water-use efficiency and drought tolerance in wheat using abscisic acid receptors (2019)
  • Author(s): Ryosuke Mega, Fumitaka Abe, June-Sik Kim, Yuuri Tsuboi, Keisuke Tanaka, Hisato Kobayashi, Yoichi Sakata, Kousuke Hanada, Hisashi Tsujimoto, Jun Kikuchi, Sean R. Cutler & Masanori Okamoto
  • Journal Title: Nature Plants Volume: 5 Issue: 2 Pages: 153-159
  • DOI: 10.1038/s41477-019-0361-8
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Distinct gene expression signatures induced by viral transactivators of different HTLV-1 subgroups that confer a different risk of HAM/TSP. (2018)
  • Author(s): Naito T, Yasunaga JI, Mitobe Y, Shirai K, Sejima H, Ushirogawa H, Tanaka Y, Nakamura T, Hanada K, Fujii M, Matsuoka M, Saito M.
  • Journal Title: Retrovirology Volume: 15 Issue: 1 Pages: 72-72
  • DOI: 10.1186/s12977-018-0454-x
  • NAID: 120006847301
  • Peer Reviewed / Open Access
• [Journal Article] Efficient anchoring of alien chromosome segments introgressed into bread wheat by new Leymus racemosus genome-based markers (2018)
  • Author(s): Edet Offiong Ukpong、Kim June-Sik、Okamoto Masanori、Hanada Kousuke、Takeda Tomoyuki、Kishii Masahiro、Gorafi Yasir Serag Alnor、Tsujimoto Hisashi
  • Journal Title: BMC Genetics Volume: 19 Issue: 1 Pages: 18-18
  • DOI: 10.1186/s12863-018-0603-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] AtPep3 is a hormone-like peptide that plays a role in the salinity stress tolerance of plants (2018)
  • Author(s): Nakaminami Kentaro、Okamoto Masanori、Higuchi-Takeuchi Mieko、Yoshizumi Takeshi、Yamaguchi Yube、Fukao Yoichiro、Shimizu Minami、Ohashi Chihiro、Tanaka Maho、Matsui Minami、Shinozaki Kazuo、Seki Motoaki、Hanada Kousuke
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 115 Issue: 22 Pages: 5810-5815
  • DOI: 10.1073/pnas.1719491115
  • Peer Reviewed
• [Journal Article] Functional divergence of duplicate genes several million years after gene duplication in Arabidopsis (2018)
  • Author(s): Hanada Kousuke、Tezuka Ayumi、Nozawa Masafumi、Suzuki Yutaka、Sugano Sumio、Nagano Atsushi J、Ito Motomi、Morinaga Shin-Ichi
  • Journal Title: DNA Research Volume: 25 Issue: 3 Pages: 327-339
  • DOI: 10.1093/dnares/dsy005
  • Peer Reviewed