| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
In this study, we aim to understand the mechanisms of heterochromatin formation in Tetrahymena DNA elimination. We found multiple HP1-like proteins localized to the heterochromatin, which regulates DNA elimination. We tethered these proteins individually to the artificially created locus and found that 4 HP1-like proteins can induce DNA elimination of the tethered site. Immunoprecipitation for the one of the HP1-like proteins Pdd1p specifically enriched the other HP1-like proteins, each of which was sufficient for the ectopic DNA elimination. These results suggest that multiple HP1-like proteins cooperatively recognize the methylated histones and form a core complex to recruit other effector proteins for DNA elimination.
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