| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
Genetic information is continuously modified by DNA damage and repair. In multicellular organisms, the genetic stability is differentially regulated depending on developmental stages and cell types. Among the various cell lineages, germline cells and stem cell systems are thought to maintain their genetic information more stably than differentiated somatic cells. However, how the genetic stability is properly coordinated with developmental programs is still not well understood. In this study, we carried out in depth comparative analyses of the genetic stability of mouse embryonic stem (ES) cells, their in vitro differentiated derivatives (ectoderm and mesendoderm etc), germline stem (GS) cells and embryonic fibroblasts etc. We obtained the proof of concept (POC) that the genetic stability of mouse embryonic stem cells can be artificially enhanced by modulating cell cycle activities and metabolic pathways while maintaining pluripotent differentiation capacities in vitro.
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