| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2022: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2019: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Outline of Final Research Achievements |
In this study, we tested inducible promoter systems and developed a system to comprehensively elucidate the functions of unknown genes using the minimal genome bacterium JCVI-syn3B. Specifically, we successfully introduced a CRISPR interference (CRISPRi) system capable of specific gene knockdown and confirmed that the tetracycline-inducible promoter system is the most effective. This system was applied to lots of functional unknown genes in JCVI-syn3B, establishing a procedure for gene function analysis. We obtained significant insights towards understanding the fundamental life functions of minimal genome bacteria.
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