| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
In Caenorhabditis elegans embryos, paternally provided organelles, including mitochondria, are eliminated by selective autophagy, and this serves as the mechanism by which mitochondrial DNA is inherited maternally. We further identified an autophagy adaptor ALLO-1 that controls this process. ALLO-1 localizes on the paternal organelles very shortly after fertilization and this localization depends on its C-terminal region. We also found that ALLO-1 physically interacts with not only LGG-1, the worm homolog of LC3/ATG8 family, but also a component of the ATG1 complex. ALLO-1 is also required for recruitment of the ATG1 complex on the substrates. Our results suggest that this ALLO-1-dependent recruitment of the ATG1 complex could explain how the autophagosome formation is initiated on substrates.
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