| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
We have succeeded in optimizing PA-Tet gene expression system. The gene expression control activity of PA-Tet can be regulated by Dox and blue light. By using PA-Tet system, we manipulated gene expression in neural stem cells and analyzed the molecular mechanism underlying processes in activation from quiescent state and neuronal differentiation. In addition, we showed neural stem cells in the adult mouse brain can also be controlled by light with the developed PA-Tet gene expression system. From these achievements, we showed that the dynamic gene expression changes in Ascl1 and Hes1 are critically important for regulations of neural stem cells.
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