| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
The pluripotency of plant cells, an important characteristic, has been suggested to be associated with circadian rhythms. However, isolation of undifferentiated cells from plants has been challenging, making it difficult to determine whether the circadian clock is involved in cell differentiation and how specific clock genes are involved. In this study, we used the Vascular cell induction culture system using Arabidopsis leaves (VISUAL) system together with time-series single-cell transcriptome analysis using glass capillaries. We found that the clock gene LUX is specifically induced at the late stage of cell division and directly regulates the expression of cell cycle and cell differentiation-related genes.
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