Establishment mechanism of local auxin accumulation to determine the initiation position of plant organogenesis

• Project/Area Number: 18H02463
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 44030:Plant molecular biology and physiology-related
• Research Institution: Kobe University
• Principal Investigator: Fukaki Hidehiro 神戸大学, 理学研究科, 教授 (80324979)
• Co-Investigator(Kenkyū-buntansha): 郷 達明 奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (80511419)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000); Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: 器官形成 / 側根形成 / オーキシン / 細胞内膜輸送 / シロイヌナズナ / 器官発生

Outline of Final Research Achievements

To elucidate the establishment mechanism of local auxin accumulation that determines the initiation of plant organogenesis, this research focused on GNOM, which encodes an ADP-ribosylation factor guanine nucleotide-exchange factor (Arf GEF) for the Arf GTPase, and conducted physiological and developmental analyses using the fwr mutant and fsp suppressor mutants that restore lateral root formation in fwr. As a result, we clarified part of the establishment mechanism of local auxin accumulation via GNOM in the initiation of lateral root formation.

Academic Significance and Societal Importance of the Research Achievements

植物器官の形成開始を決定する局所的なオーキシン蓄積の確立機構を理解することは、植物生理学・発生学分野の進展に大きく貢献することが期待される。本研究は、その機構に関わる新たな細胞内膜輸送系を明らかにする点でインパクトがあるとともに、器官の形成開始位置を調節して農作物・園芸品種の器官形成パターンを改良する技術につながる点においても創造性がある。

Research Products

• [Journal Article] Tissue growth constrains root organ outlines into an isometrically scalable shape (2021)
  • Author(s): Fujiwara Motohiro、Goh Tatsuaki、Tsugawa Satoru、Nakajima Keiji、Fukaki Hidehiro、Fujimoto Koichi
  • Journal Title: Development Volume: 148 Issue: 4 Pages: 196253-196253
  • DOI: 10.1242/dev.196253
  • NAID: 120007028967
  • Peer Reviewed / Open Access
• [Journal Article] Root-knot nematodes induce gall formation by recruiting developmental pathways of post-embryonic organogenesis and regeneration to promote pluripotency (2020)
  • Author(s): Olmo, R., Cabrera, J., Diaz-Manzano, F., Ruiz-Ferrer, V., Barcala, M., Ishida, T., Garcia A., Andres, M., Lara, S.R., Verdugo, I., Ochoa, M., Fukaki, H., del Pozo, J. C., Moreno-Risueno, M., Kyndt, T., Gheysen L., Fenoll, C., Sawa, S., and Escobar, C.
  • Journal Title: New Phytologist Volume: 227 Issue: 1 Pages: 210-215
  • DOI: 10.1111/nph.16521
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Blue Light Regulates Phosphate Deficiency-Dependent Primary Root Growth Inhibition in Arabidopsis (2020)
  • Author(s): Yeh Chuan-Ming, Kobayashi Koichi, Fujii Sho, Fukaki Hidehiro, Mitsuda Nobutaka, Ohme-Takagi Masaru
  • Journal Title: Frontiers in Plant Science Volume: 10 Pages: 1803-1803
  • DOI: 10.3389/fpls.2019.01803
  • Peer Reviewed / Open Access
• [Journal Article] Peptide-Receptor Signaling Controls Lateral Root Development (2020)
  • Author(s): Jourquin J, Fukaki H, Beeckman T.
  • Journal Title: Plant Physiol. Volume: 182 Issue: 4 Pages: 1645-1656
  • DOI: 10.1104/pp.19.01317
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 根が分岐する間隔を調節する低分子分泌ペプチド (2020)
  • Author(s): 豊倉 浩一, 郷 達明, 深城 英弘
  • Journal Title: 化学と生物 Volume: 58 Pages: 322-324
  • Peer Reviewed
• [Journal Article] Editorial: Root Branching: From Lateral Root Primordium Initiation and Morphogenesis to Function (2019)
  • Author(s): Dubrovsky JG, Fukaki H, Laplaze L, Laskowski M.
  • Journal Title: Front Plant Sci. Volume: 10 Pages: 1462-1462
  • DOI: 10.3389/fpls.2019.01462
  • NAID: 120006823992
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cytoskeleton Dynamics Are Necessary for Early Events of Lateral Root Initiation in Arabidopsis (2019)
  • Author(s): Vilches Barro A, Stockle D, Thellmann M, Ruiz-Duarte P, Bald L, Louveaux M, von Born P, Denninger P, Goh T, Fukaki H, Vermeer JEM, Maizel A.
  • Journal Title: Curr Biol. Volume: 29 Issue: 15 Pages: 2443-2454
  • DOI: 10.1016/j.cub.2019.06.039
  • NAID: 120006823993
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lateral root initiation requires the sequential induction of transcription factors LBD16 and PUCHI in Arabidopsis thaliana (2019)
  • Author(s): Goh Tatsuaki、Toyokura Koichi、Yamaguchi Nobutoshi、Okamoto Yoshie、Uehara Takeo、Kaneko Shutaro、Takebayashi Yumiko、Kasahara Hiroyuki、Ikeyama Yoshifumi、Okushima Yoko、Nakajima Keiji、Mimura Tetsuro、Tasaka Masao、Fukaki Hidehiro
  • Journal Title: New Phytologist Volume: 224 Issue: 2 Pages: 749-760
  • DOI: 10.1111/nph.16065
  • Peer Reviewed / Open Access
• [Journal Article] Reactive oxygen species and reactive carbonyl species constitute a feed‐forward loop in auxin signaling for lateral root formation (2019)
  • Author(s): Biswas Md. Sanaullah、Fukaki Hidehiro、Mori Izumi C.、Nakahara Kazuha、Mano Jun'ichi
  • Journal Title: The Plant Journal Volume: 100 Issue: 3 Pages: 536-548
  • DOI: 10.1111/tpj.14456
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] PUCHI regulates very long chain fatty acid biosynthesis during lateral root and callus formation (2019)
  • Author(s): Trinh DC, Lavenus J, Goh T, Boutte Y, Drogue Q, Vaissayre V, Tellier F, Lucas M, Voss U, Gantet P, Faure JD, Dussert S, Fukaki H, Bennett MJ, Laplaze L, Guyomarc'h S.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 116 Issue: 28 Pages: 14325-14330
  • DOI: 10.1073/pnas.1906300116
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mitochondrial Pyruvate Dehydrogenase Contributes to Auxin-Regulated Organ Development (2019)
  • Author(s): Ohbayashi I, Huang S, Fukaki H, Song X, Sun S, Morita MT, Tasaka M, Millar AH, Furutani M.
  • Journal Title: Plant Physiol. Volume: 180 Issue: 2 Pages: 896-909
  • DOI: 10.1104/pp.18.01460
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 側根形成の制御機構に関する研究の新展開 (2019)
  • Author(s): 深城英弘, 豊倉浩一, 郷 達明
  • Journal Title: 植物の生長調節 Volume: 54 Pages: 129-136
  • NAID: 130008060490
  • Peer Reviewed
• [Journal Article] Lateral inhibition by a peptide hormone-receptor cascade during Arabidopsis lateral root founder cell formation. (2019)
  • Author(s): Toyokura K, Goh T, Shinohara H, Shinoda A, Kondo Y, Okamoto Y, Uehara T, Fujimoto K, Okushima Y, Ikeyama Y, Nakajima K, Mimura T, Tasaka M, Matsubayashi Y and Fukaki H.
  • Journal Title: Developmental Cell Volume: 48 Issue: 1 Pages: 64-75
  • DOI: 10.1016/j.devcel.2018.11.031
  • Peer Reviewed / Open Access
• [Journal Article] Root branching toward water involves posttranslational modification of transcription factor ARF7 (2018)
  • Author(s): Orosa-Puente B、Leftley N、von Wangenheim D、Banda J、Srivastava AK.、Hill K、Truskina J、Bhosale R、Morris E、Srivastava M、Kmpers B、Goh T、Fukaki H、Vermeer JEM.、Vernoux T、Dinneny JR.、French AP.、Bishopp A、Sadanandom A、Bennett MJ.
  • Journal Title: Science Volume: 362 Issue: 6421 Pages: 1407-1410
  • DOI: 10.1126/science.aau3956
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Jasmonic acid inhibits auxin-induced lateral rooting independently of the CORONATINE INSENSITIVE 1 receptor (2018)
  • Author(s): Ishimaru Yasuhiro、Hayashi Ken-ichiro、Suzuki Takeshi、Fukaki Hidehiro、Prusinska Justyna、Meesters Christian、Quareshy Mussa、Egoshi Yuusuke、Matsuura Hideyuki、Takahashi Kosaku、Kato Nobuki、Kombrink Erich、Napier Richard M.、Hayashi Kengo、Ueda Minoru
  • Journal Title: Plant Physiology Volume: 177 Issue: 4 Pages: 1704-1716
  • DOI: 10.1104/pp.18.00357
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 側根形成能が顕著に低下するシロイヌナズナの変異体 fewer roots (fwr) のサプレッサー変異体 fewer roots suppressor 1 (fsp1)の解析 (2021)
  • Author(s): 後藤千恵子，池上聡，郷達明，笠原博幸，近藤侑貴，石崎公庸，三村徹郎，深城英弘
  • Organizer: 第62回日本植物生理学会年会
• [Presentation] 発光レポーター遺伝子を用いた側根プレパターニング変異体の単離と解析 (2021)
  • Author(s): 小笹綾香，後藤千恵子，郷達明，近藤侑貴，石崎公庸，三村徹郎，深城英弘
  • Organizer: 第62回日本植物生理学会年会
• [Presentation] 側根形成を負に制御するTOLS2ペプチド応答性が変化する変異体の解析 (2020)
  • Author(s): 西丸陸, 青木優佳, 豊倉浩一, 篠田明徳, 郷達明, 石崎公庸, 三村徹郎, 深城英弘
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] 発光レポーター遺伝子を用いた側根プレパターニング変異体の単離と解析 (2020)
  • Author(s): 小笹綾香, 郷達明, 石崎公庸, 三村徹郎, 深城英弘
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] 側根形成におけるオーキシン作用の二面性 (2020)
  • Author(s): 深城英弘
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] TOLS2/PIPL3 peptide-RLK7 receptor cascade regulates lateral root spacing during Arabidopsis lateral root founder cell formation (2019)
  • Author(s): Hidehiro Fukaki
  • Organizer: 第23回国際植物生長物質会議
  • Int'l Joint Research / Invited
• [Presentation] レポーター遺伝子を用いたシロイヌナズナ側根プレパターニング機構の解析 (2019)
  • Author(s): 小笹 綾香, 郷 達明, 石崎 公庸, 三村 徹郎, 深城 英弘
  • Organizer: 日本植物学会第83回大会
• [Presentation] TOLS2ペプチドによる側根形成関連遺伝子の発現制御に異常を示す変異体の解析 (2019)
  • Author(s): 西丸 陸, 青木 優佳, 豊倉 浩一, 篠田 明徳, 郷 達明, 石崎 公庸, 三村徹郎, 深城 英弘
  • Organizer: 日本植物学会第83回大会
• [Presentation] シロイヌナズナとミチタネツケバナにおける根の皮層形成の分子機構の比較 (2019)
  • Author(s): 豊倉浩一, 郷達明, 坂根雅人, Yrjo Helariutta, 柿本辰男, 工藤洋, 深城英弘
  • Organizer: 第60回日本植物生理学会年会 2019年3月
• [Presentation] 西丸陸, 青木優佳, 豊倉浩一, 篠田明徳, 郷達明, 石崎公庸, 三村徹郎, 深城英弘 (2019)
  • Author(s): シロイヌナズナ側根形成を抑制するTOLS2 ペプチドによる遺伝子の発現制御に異常を示す変異体の解析
  • Organizer: 第60回日本植物生理学会年会 2019年
• [Presentation] シロイヌナズナ側根形成能が顕著に低下するfewer roots変異体のサプレッサー変異体の解析 (2018)
  • Author(s): 田井中 芳樹, 郷 達明, 石崎 公庸, 三村 徹郎, 深城 英弘
  • Organizer: 日本植物学会第82回大会 2018年9月
• [Presentation] シロイヌナズナ側根形成を抑制するTOLS2ペプチドに対する応答異常変異体の解析 (2018)
  • Author(s): 西丸 陸, 青木 優佳, 雨川 智美, 豊倉 浩一, 篠田 明徳, 郷 達明, 石崎 公庸, 三村 徹郎, 深城 英弘
  • Organizer: 日本植物学会第82回大会 2018年9月
• [Remarks] 神戸大学大学院理学研究科生物学専攻・深城研究室ウェブサイト
  • URL: http://www.research.kobe-u.ac.jp/fsci-fukaki/fukaki/fukaki_laboratory.html
• [Remarks] 神戸大学大学院理学研究科生物学専攻・理学部生物学科ウェブサイト（教員紹介）
  • URL: http://www.edu.kobe-u.ac.jp/fsci-biol/faculty/fukaki.html