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Multi-dimensional studies on structure and dynamics plasticity of the VNS-regulon governing xylem cell differentiation

Research Project

Project/Area Number 18H02466
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionNara Institute of Science and Technology

Principal Investigator

Demura Taku  奈良先端科学技術大学院大学, デジタルグリーンイノベーションセンター, 教授 (40272009)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Keywordsメタボローム解析 / 化学生物学的解析 / 順遺伝学 / ユビキチン化 / 進化的変遷 / 木部細胞分化 / 転写ネットワーク / マルチオミクス / HDAC阻害剤 / 蛍光相関分光法 / 道管 / 遺伝子発現制御ネットワーク / 進化 / オミクス解析 / ケミカルバイオロジー
Outline of Final Research Achievements

In this study, we analyzed in detail the structure and dynamic plasticity of the VNS regulon, a gene expression regulatory system that plants have evolutionarily developed to optimize the function of water conduction and body-support by vascular xylem cells, in a multilayered approach. The results revealed that 1) degradation of a group of proteins is important for the optimization of xylem cell functions, 2) the cell cycle and intracellular environment are deeply involved in the regulation of xylem cell differentiation, and 3) the basic structure of the VNS regulon is conserved in a moss (Sphagnum palustre) and a conifer (Pinus taeda).

Academic Significance and Societal Importance of the Research Achievements

本研究が対象とする木部細胞は木質バイオマスの本体であり、本研究で得られた成果をもとに木質バイオマスの高度利用(バイオエタノール変換、高エネルギー化)に向けた木質改良につながる点で社会的な意義をもつ。また、本研究が扱う「VNSレギュロン」は植物における遺伝子発現制御システムの代表例であり、この解析で得られた知見と解析手法が他の様々な局面で起動するレギュロンの解析に転用可能である点で学術的な意義は大きい。

Report

(4 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (37 results)

All 2020 2019 2018 Other

All Int'l Joint Research (11 results) Journal Article (13 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 13 results,  Open Access: 10 results) Presentation (12 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Funded Workshop (1 results)

  • [Int'l Joint Research] ブリティッシュコロンビア大学(カナダ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Universite de Picardie Jules Verne(フランス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] ペンシルバニア州立大学/ジョージア大学(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] 浙江農林大学/中国林業科学院(中国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] MPI(ドイツ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] ブリティッシュコロンビア大学(カナダ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] CNRS(フランス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] 浙江農林大学/中国林業科学院(中国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] MPI(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ブリティッシュコロンビア大学(カナダ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] MPI(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Impact of abiotic stress on the regulation of cell wall biosynthesis in <i>Populus trichocarpa</i>2020

    • Author(s)
      Hori Chiaki、Yu Xiang、Mortimer Jenny C.、Sano Ryosuke、Matsumoto Tomoko、Kikuchi Jun、Demura Taku、Ohtani Misato
    • Journal Title

      Plant Biotechnology

      Volume: 37 Issue: 3 Pages: 273-283

    • DOI

      10.5511/plantbiotechnology.20.0326a

    • NAID

      130007907472

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2020-09-25
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Hechtian Strands Transmit Cell Wall Integrity Signals in Plant Cells2020

    • Author(s)
      Yoneda Arata、Ohtani Misato、Katagiri Daisuke、Hosokawa Yoichiroh、Demura Taku
    • Journal Title

      Plants

      Volume: 9 Issue: 5 Pages: 604-604

    • DOI

      10.3390/plants9050604

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Isolation of dominant Arabidopsis seive mutants defective in VND7-induced xylem vessel cell differentiation2020

    • Author(s)
      Pawittra Phookaew、Suzuki Takaomi、Kawabe Harunori、Takebayashi Arika、Demura Taku、Ohtani Misato
    • Journal Title

      Plant Biotechnology

      Volume: 37 Issue: 3 Pages: 311-318

    • DOI

      10.5511/plantbiotechnology.20.0427a

    • NAID

      130007907448

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure and Biomechanics during Xylem Vessel Transdifferentiation in Arabidopsis thaliana2020

    • Author(s)
      Roumeli Eleftheria、Ginsberg Leah、McDonald Robin、Spigolon Giada、Hendrickx Rodinde、Ohtani Misato、Demura Taku、Ravichandran Guruswami、Daraio Chiara
    • Journal Title

      Plants

      Volume: 9 Issue: 12 Pages: 1715-1715

    • DOI

      10.3390/plants9121715

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Plant-specific Dof transcription factors VASCULAR-RELATED DOF1 and VASCULAR-RELATED DOF2 regulate vascular cell differentiation and lignin biosynthesis in Arabidopsis2020

    • Author(s)
      Ramachandran Vasagi、Tobimatsu Yuki、Masaomi Yamamura、Sano Ryosuke、Umezawa Toshiaki、Demura Taku、Ohtani Misato
    • Journal Title

      Plant Molecular Biology

      Volume: 104 Issue: 3 Pages: 263-281

    • DOI

      10.1007/s11103-020-01040-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis FLYING SAUCER 2 Functions Redundantly with FLY1 to Establish Normal Seed Coat Mucilage2020

    • Author(s)
      Tadashi Kunieda、Ikuko Hara-Nishimura、Taku Demura、George W. Haughn
    • Journal Title

      Plant and Cell Physiology

      Volume: 61 Issue: 2 Pages: 308-317

    • DOI

      10.1093/pcp/pcz195

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Interspecific signaling between the parasitic plant and the host plants regulate xylem vessel cell differentiation in haustoria of Cuscuta campestris.2020

    • Author(s)
      Kaga Y., Yokoyama R., Sano, R., Ohtani, M., Demura, T., Kuroha, T., Shinohara, N., Nishitani, K..
    • Journal Title

      Frontiers in Plant Science

      Volume: 11 Pages: 193-193

    • DOI

      10.3389/fpls.2020.00193

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Creating vessel elements in vitro: Towards a comprehensive understanding of the molecular basis of xylem vessel element differentiation2019

    • Author(s)
      Tan Tian Tian、Demura Taku、Ohtani Misato
    • Journal Title

      Plant Biotechnology

      Volume: 36 Issue: 1 Pages: 1-6

    • DOI

      10.5511/plantbiotechnology.18.1119b

    • NAID

      130007628755

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2019-03-25
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Affinity‐based high‐resolution analysis of DNA binding by VASCULAR‐RELATED NAC‐DOMAIN7 via fluorescence correlation spectroscopy2019

    • Author(s)
      Tamura Taizo、Endo Hitoshi、Suzuki Atsunobu、Sato Yutaka、Kato Ko、Ohtani Misato、Yamaguchi Masatoshi、Demura Taku
    • Journal Title

      The Plant Journal

      Volume: 100 Issue: 2 Pages: 298-313

    • DOI

      10.1111/tpj.14443

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Progression of Xylem Vessel Cell Differentiation is Dependent on the Activity Level of VND7 in Arabidopsis thaliana2019

    • Author(s)
      Hirai Risaku、Higaki Takumi、Takenaka Yuto、Sakamoto Yuki、Hasegawa Junko、Matsunaga Sachihiro、Demura Taku、Ohtani Misato
    • Journal Title

      Plants

      Volume: 9 Issue: 1 Pages: 39-39

    • DOI

      10.3390/plants9010039

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes2019

    • Author(s)
      Saelim Laddawan、Akiyoshi Nobuhiro、Tan Tian Tian、Ihara Ayumi、Yamaguchi Masatoshi、Hirano Ko、Matsuoka Makoto、Demura Taku、Ohtani Misato
    • Journal Title

      Journal of Plant Research

      Volume: 132 Issue: 1 Pages: 117-129

    • DOI

      10.1007/s10265-018-1074-1

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The quest for transcriptional hubs of lignin biosynthesis: beyond the NAC-MYB-gene regulatory network model2019

    • Author(s)
      Ohtani Misato、Demura Taku
    • Journal Title

      Current Opinion in Biotechnology

      Volume: 56 Pages: 82-87

    • DOI

      10.1016/j.copbio.2018.10.002

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Patterned deposition of xylan and lignin is independent from the secondary wall cellulose of Arabidopsis xylem vessels2018

    • Author(s)
      Takenaka Yuto、Watanabe Yoichiro、Schuetz Mathias、Unda Faride、Hill Joseph Lee、Phookaew Pawittra、Yoneda Arata、Mansfield Shawn D.、Samuels A. Lacey、Ohtani Misato、Demura Taku
    • Journal Title

      The Plant Cell

      Volume: 30 Issue: 11 Pages: 2663-2676

    • DOI

      10.1105/tpc.18.00292

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] シロイヌナズナ木部道管様細胞において原形質分離は二次細胞壁形成を抑制する2020

    • Author(s)
      水元康裕, 國枝正, 出村拓
    • Organizer
      2020年度(第9回)近畿植物学会講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 原子間力顕微鏡を用いた二次細胞壁形成過程における細胞の力学的強度変化の解析2020

    • Author(s)
      川村純平, 國枝正, 岸田佳祐, 秋田絵理, 細川陽一郎, 出村拓
    • Organizer
      2020年度(第9回)近畿植物学会講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナ花茎における細胞壁の変化がヤング率に与える影響2020

    • Author(s)
      仲尾真男, 中田未友希, 出村拓
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Tracking CELLULOSE SYNTHASE movement during secondary cell wall formation2020

    • Author(s)
      石尾寛乃, 渡邊洋一郎, 出村拓
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナ木部道管細胞分化における細胞の力学的強度変化の解析2020

    • Author(s)
      川村純平, 國枝正, 岸田佳祐, 秋田絵理, 岡野和宣, 細川陽一郎, 出村拓
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 木部道管様細胞の二次壁形成における一次細胞壁の役割2020

    • Author(s)
      水元康裕, 國枝正, 出村拓
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] AC転写因子VND7は道管細胞においてユビキチンE3リガーゼFLYの発現を制御する2020

    • Author(s)
      國枝正, 地福海月, George W. Haughn, 西村いくこ, 出村拓
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 生物学から見たCNF生合成のしくみ2020

    • Author(s)
      大谷美沙都, 出村拓
    • Organizer
      日本化学会第100回春季大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Functional Conservation of VNS NAC Transcription Factors That Regulate Cell Wall Formation and Programmed Cell Death in Land Plants2019

    • Author(s)
      Taku Demura
    • Organizer
      Marchantia Workshop 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional Analysis of Polygalacturonases During Water-Conducting Cell Formation Required for Plant Adaptation to Land2019

    • Author(s)
      Taku Demura
    • Organizer
      Carbohydrate-Active Enzymes for Glycan Conversions (Gordon Research Conference)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] EVOLUTIONAL CHANGES IN CIS-ELEMENT BINDING AFFINITY OF VNS FAMILY TRANSCRIPTION FACTORS, MASTER REGULATORS OF SECONDARY CELL WALL FORMATION2019

    • Author(s)
      Nobuhiro Akiyoshi, Taizo Tamura, Misato Ohtani and Taku Demura
    • Organizer
      XV Cell Wall Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 木部道官細胞分化におけるユビキチンE3リガーゼFLYの発現制御機構の解析2019

    • Author(s)
      地福海月, 國枝正, George W. Haughn, 西村いくこ and 出村拓
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Funded Workshop] France-Japan Plant Cell Wall Workshop "Pectin Lovers Meeting"2019

    • Related Report
      2019 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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