Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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Outline of Final Research Achievements |
Stomata open in response to light, facilitating the uptake of CO2 for photosynthetic carbon fixation. Phototropins, plant-specific blue light photoreceptor kinases, phosphorylate their substrate BLUS1 kinase, leading to activation of the plasma membrane H+-ATPase and stomatal opening. In this study, we revealed the detailed regulatory mechanisms of BLUS1 kinase activity and showed that the decrease of intercellular CO2 concentration by photosynthesis in addition to the BLUS1-mediated H+-ATPase activation is necessary for light-dependent stomatal opening. Furthermore, we showed that phototropins mediate stomatal closure as well as stomatal opening in response to blue light, thereby strictly controlling stomatal aperture. We also found that autophagy sustains light-dependent stomatal opening through regulating ROS homeostasis in guard cells.
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