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Signaling network of phototropin-mediated stomatal opening

Research Project

Project/Area Number 18H02468
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionYamaguchi University

Principal Investigator

Takemiya Atsushi  山口大学, 大学院創成科学研究科, 准教授 (80448406)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywordsフォトトロピン / 気孔 / 青色光 / シグナル伝達 / タンパク質リン酸化 / 気孔開口
Outline of Final Research Achievements

Stomata open in response to light, facilitating the uptake of CO2 for photosynthetic carbon fixation. Phototropins, plant-specific blue light photoreceptor kinases, phosphorylate their substrate BLUS1 kinase, leading to activation of the plasma membrane H+-ATPase and stomatal opening. In this study, we revealed the detailed regulatory mechanisms of BLUS1 kinase activity and showed that the decrease of intercellular CO2 concentration by photosynthesis in addition to the BLUS1-mediated H+-ATPase activation is necessary for light-dependent stomatal opening. Furthermore, we showed that phototropins mediate stomatal closure as well as stomatal opening in response to blue light, thereby strictly controlling stomatal aperture. We also found that autophagy sustains light-dependent stomatal opening through regulating ROS homeostasis in guard cells.

Academic Significance and Societal Importance of the Research Achievements

本研究ではこれまで未解明であった光シグナルと光合成による相乗的な気孔開口のメカニズムに加え、フォトトロピンによる気孔閉鎖やオートファジーによる気孔開口制御など、これまで想像されていなかった気孔開閉制御機構の存在を初めて示した。今回見出された新たな気孔開閉制御のメカニズムを分子レベルで解明することで、高い生産性や環境適応能力を備えた農作物の開発に繋がり、将来的に地球規模での環境・食料・エネルギー問題の解決に貢献することが期待される。

Report

(4 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (27 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (18 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Remarks (2 results)

  • [Int'l Joint Research] ETH Zurich(スイス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Glasgow(英国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Henan University(中国)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] A BLUS1 kinase signal and a decrease in intercellular CO2 concentration are necessary for stomatal opening in response to blue light2021

    • Author(s)
      Hosotani Sakurako、Yamauchi Shota、Kobayashi Haruki、Fuji Saashia、Koya Shigekazu、Shimazaki Ken-ichiro、Takemiya Atsushi
    • Journal Title

      The Plant Cell

      Volume: - Issue: 5 Pages: 1813-1827

    • DOI

      10.1093/plcell/koab067

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] CIPK23 regulates blue light-dependent stomatal opening in Arabidopsis thaliana.2020

    • Author(s)
      1.Inoue S, Kaiserli E, Zhao X, Waksman T, Takemiya A, Okumura M, Takahashi H, Seki M, Shinozaki K, Endo Y, Sawasaki T, Kinoshita T, Zhang X, Christie JM, Shimazaki K
    • Journal Title

      The Plant Journal

      Volume: 104 Issue: 3 Pages: 679-692

    • DOI

      10.1111/tpj.14955

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Guard Cell Starch Degradation Yields Glucose for Rapid Stomatal Opening in Arabidopsis2020

    • Author(s)
      Flutsch Sabrina、Wang Yizhou、Takemiya Atsushi、Vialet-Chabrand Silvere R. M.、Klejchov Martina、Nigro Arianna、Hills Adrian、Lawson Tracy、Blatt Michael R.、Santelia Diana
    • Journal Title

      The Plant Cell

      Volume: 32 Issue: 7 Pages: 2325-2344

    • DOI

      10.1105/tpc.18.00802

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Autophagy controls reactive oxygen species homeostasis in guard cells that is essential for stomatal opening2019

    • Author(s)
      Yamauchi, S., Mano, S., Oikawa, K., Hikino, K., Teshima, M.K., Kimori, Y., Nishimura, M., Shimazaki, K., and Takemiya, A.
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 116 Issue: 38 Pages: 19187-19192

    • DOI

      10.1073/pnas.1910886116

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] 青色光による気孔開口には BLUS1を介したH+-ATPaseの活性化と光合成による葉内CO2濃度の低下が必要である2021

    • Author(s)
      細谷桜子, 山内翔太, 小林遥貴, 冨士彩紗, 古屋繁一, 島崎研一郎, 武宮淳史
    • Organizer
      第62回日本植物生理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 青色光によるBEC1のリン酸化は孔辺細胞のデンプン分解を誘導し気孔開口を促進する2021

    • Author(s)
      山内翔太, 杉山直幸, 島崎研一郎, 武宮淳史
    • Organizer
      第62回日本植物生理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] amiRNAスクリーニングによるフォトトロピン情報伝達の新規制御因子の同定2021

    • Author(s)
      三藤有紗, 小林遥貴, 松本理緒, 武宮淳史
    • Organizer
      第62回日本植物生理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 青色光による気孔開口におけるBLUS1キナーゼドメインの機能解析2021

    • Author(s)
      冨士彩紗, 田原京佳, 山内翔太, 細谷桜子, 岡島公司, 島崎研一郎, 武宮淳史
    • Organizer
      第62回日本植物生理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 赤外線サーモグラフィを用いた植物の気孔応答の研究2020

    • Author(s)
      武宮淳史
    • Organizer
      Biothermology Workshop 2020
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] オートファジーは孔辺細胞のROSホメオスタシスを制御することで青色光依存の気孔開口を可能にする2020

    • Author(s)
      山内翔太, 真野昌二, 及川和聡, 曳野和美, 手島康介, 木森義隆, 西村幹夫, 島崎研一郎, 武宮 淳史
    • Organizer
      第61回日本植物生理学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 活性カルボニル種はROSシグナル伝達を仲介し青色光による気孔開口を阻害する2020

    • Author(s)
      村上奈々香, 真野純一, 武宮淳史
    • Organizer
      第61回日本植物生理学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] E3ユビキチンリガーゼBEC1はデンプン分解を誘導し気孔開口を促進させる2019

    • Author(s)
      山内翔太, 杉山直幸, 島崎研一郎, 武宮淳史
    • Organizer
      生物系三学会中四国支部会(広島大会)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 活性カルボニル種(RCS)は青色光による気孔開口を阻害する2019

    • Author(s)
      村上奈々香, 真野純一, 武宮淳史
    • Organizer
      生物系三学会中四国支部会(広島大会)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 孔辺細胞において青色光に応じてリン酸化されるプロテインキナーゼの機能解析2019

    • Author(s)
      冨士彩紗, 武宮淳史
    • Organizer
      生物系三学会中四国支部会(広島大会)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Signaling network of phototropin-regulated stomatal opening2019

    • Author(s)
      Atsushi Takemiya
    • Organizer
      Japan-Taiwan Plant Biology 2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 気孔孔辺細胞において青色光に応答してリン酸化されるE3ユビキチンリガーゼの機能解析2019

    • Author(s)
      山内翔太、杉山直幸、島崎研一郎、武宮淳史
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 孔辺細胞青色光シグナル伝達におけるBLUS1のC末端領域の機能解析2019

    • Author(s)
      細谷桜子、古屋繁一、島崎研一郎、武宮淳史
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Molecular mechanism of blue light signaling for stomatal opening2018

    • Author(s)
      Atsushi Takemiya, Shota Yamauchi, Asami Hiyama, Ken-ichiro Shimazaki
    • Organizer
      Japan-Finland Seminar 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 気孔孔辺細胞において青色光依存的にリン酸化される情報伝達因子の機能解析2018

    • Author(s)
      山内翔太、武宮淳史
    • Organizer
      生物系三学会中四国支部会(山口大会)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 青色光による気孔開口の必須因子BLUS1のC末端領域の機能解析2018

    • Author(s)
      細谷桜子、古屋繁一、島崎研一郎、武宮淳史
    • Organizer
      生物系三学会中四国支部会(山口大会)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 光によらず暗所で気孔が開口するシロイヌナズナG7-9D変異体の表現型解析2018

    • Author(s)
      楢原康太、石川和弥、島崎研一郎、武宮淳史
    • Organizer
      生物系三学会中四国支部会(山口大会)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 青色光特異的な気孔開口に異常を示すシロイヌナズナ突然変異体の単離と機能解析2018

    • Author(s)
      小林遥貴、武宮淳史
    • Organizer
      生物系三学会中四国支部会(山口大会)
    • Related Report
      2018 Annual Research Report
  • [Remarks] 光による気孔開口制御の仕組みを解明 ~高い光合成能力とストレス耐性能力を併せ持つ植物の開発に期待~

    • URL

      http://www.yamaguchi-u.ac.jp/weeklynews/_8427/_9082.html

    • Related Report
      2020 Annual Research Report
  • [Remarks] オートファジーは活性酸素の蓄積を抑え気孔開口を可能にする!

    • URL

      http://www.yamaguchi-u.ac.jp/weeklynews/_7735/_7989.html

    • Related Report
      2019 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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