| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2021: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2020: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2018: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
|
|---|
| Outline of Final Research Achievements |
Centrosomes form spindle microtubules and the centrosomal microtubules plays in role of chromosome capture and alignment in animal cells. The centrosomes are lost in plant cells, and how spindle microtubules are formed and how chromosomes are captured by them are unknown. In this study, we visualize behavior of the nuclear envelope, microtubules, and chromosomes, and the interaction between microtubules and chromosomes by live imaging. Furthermore, microtubules were double-labeled with mScarlet-i and msGFP, and photobleaching experiments were performed to analyze rates of microtubule polymerization and depolymerization. It was found that microtubule polymerization shifts to the inside of the fragmented nuclear envelope. Since microtubules originating from the centromere could not be detected, it was considered that induction of microtubules by chromosomes is not involved in microtubule polymerization.
|
