Molecular mechanism of compound eye dependent photic entrainment in insect circadian clocks
| Project/Area Number |
18H02480
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 44050:Animal physiological chemistry, physiology and behavioral biology-related
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| Research Institution | Okayama University |
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Principal Investigator |
Tomioka Kenji 岡山大学, 自然科学研究科, 教授 (30136163)
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| Co-Investigator(Kenkyū-buntansha) |
吉井 大志 岡山大学, 自然科学研究科, 准教授 (50611357)
守山 禎之 川崎医科大学, 医学部, 助教 (30707394)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Keywords | 概日リズム / 概日時計 / 光同調 / 時計遺伝子 / 複眼 / RNAi / 昆虫 |
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| Outline of Final Research Achievements |
The circadian clock sets the peak time for various physiological functions by synchronizing with the daily environmental cycle. In the present study, we have elucidated the molecular mechanism of compound-eye dependent light entrainment and found that light information from the compound eye induces c-fosB expression in the optic lobe, which in turn induces Brwd3, Fbxl3, Fbxl 4, Fbxl 5, Fbxl 7, Fbxl 13, and Fbxl 16 downstream of c-fosB. Their RNAi markedly suppressed light entrainment of behavioral rhythms, in both advance and delay shifts, and CRY2 protein increased during the dark period but decreased with light exposure. The light-dependent decrease in CRY2 protein was suggested to be induced by light-dependent ubiquitination by Brwd3 and Fbxls. A similar mechanism was suggested to be involved in firebrats.
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| Academic Significance and Societal Importance of the Research Achievements |
概日時計はヒトを含めて動物の日周リズムを制御しており、その最も重要な働きは光周期に同調し、各種の生理機能のピークを適切な時刻に設定することにある。昆虫概日時計の光同調機構は、キイロショウジョウバエでクリプトクロム（CRY）に依存した機構が詳しく解析されている。しかし、この系は例外的であり、多くの昆虫は複眼を光同調に使っている。実際、ショウジョウバエでも、CRYの機能欠損系統が光周期に同調することが知られており、この場合には複眼が光受容器として働いている。従って、複眼を経由する光同調機構を解明することは、昆虫概日時計の光同調機構を理解する上で最も重要な課題である。
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] The role of clockwork orange in the circadian clock of the cricket Gryllus bimaculatus2020
Author(s)
Tomiyama, Y., Shinohara, T., Matsuka, M., Bando, T., Mito, T. & Tomioka, K.
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Journal Title
Zoological Letters
Volume: 6
Issue: 1
Pages: 12-12
DOI
NAID
Related Report
Peer Reviewed / Open Access
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