| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2021: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Outline of Final Research Achievements |
The whole genome assembly of habu individuals from four populations, Amami (Protobothrops flavoviridis), Okinawa (P. flavoviridis), Kodakara (P. tokarensis) and Iriomote (P. elegans) revealed different structures in gene clusters of venom protein gene families such as metalloproteinase and C-type lectin genes (supported by JSPS KAKENHI Grant Number 22H04925 (PAGS)). We also obtained mtDNA sequences from 150 individuals from 14 populations and evaluated the genetic divergence and migrations among the populations. 2D analyses of venoms from 47 individuals from 9 populations showed different expression pattern between populations as well as within populations, while RNAseq of venom glands showed no clear difference in expression levels of venom protein genes between populations. These observations suggest that the difference in protein expression levels in venoms can be attributed to genomic, translational and post-translational levels rather than transcriptional levels.
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