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Elucidating coastal ecosystem structure using compound-specific isotope analysis of amino acids

Research Project

Project/Area Number 18H02513
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 45040:Ecology and environment-related
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

ISHIKAWA Naoto  国立研究開発法人海洋研究開発機構, 海洋機能利用部門(生物地球化学センター), 研究員 (80609389)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2020: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Keywords放射性炭素 / 窒素同位体比 / グルタミン酸 / フェニルアラニン / メチオニン
Outline of Final Research Achievements

The aim of this study was to understand the structure of coastal ecosystems using radiocarbon (Δ14C) and amino acid nitrogen isotopic compositions (δ15N). First, the research methodology was established. Second, we showed that the dissolved organic matter in streams is a mixture of organic carbons with different turnover times. Third, we discussed the utility of Δ14C as a proxy of carbon cycling. Furthermore, Δ14C and amino acid δ15N were combined and applied for off Sanriku ecosystems, where a variety of fish communities is supported by a mixture of diet sources derived from different ocean currents. We also revealed that the integrated trophic position (iTP) of marine mesozooplankton communities was negatively correlated with species diversity.

Academic Significance and Societal Importance of the Research Achievements

海洋沿岸域は漁業資源、レクリエーションといったサービスをもたらすため、人類にとって最も身近な生態系の1つであると同時に、人為的な環境破壊の影響を受けやすい。本研究によって、生態系解析における放射性炭素とアミノ酸の窒素同位体比の有効性が示された。この方法論は、国連の「持続可能な開発目標(SDGs)」や、ユネスコの「持続可能な開発のための国連海洋科学10年」を進める上で、さまざまな科学的知見を得るための重要な基盤技術になるものと期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (28 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (6 results) Journal Article (15 results) (of which Int'l Joint Research: 12 results,  Peer Reviewed: 15 results,  Open Access: 10 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (2 results)

  • [Int'l Joint Research] University of Minnesota, St. Paul/University of California, Berkeley(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ETH Zurich(スイス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Yale University/University of Washington/University of Minnesota(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ETH Zurich(スイス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Uppsala University(スウェーデン)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] National Taiwan University(台湾)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Influences of ocean currents on the diets of demersal fish communities in the western North Pacific revealed by their muscle carbon and nitrogen isotopic compositions2021

    • Author(s)
      Naoto F Ishikawa, Nanako O Ogawa, Yoshito Chikaraishi, Moto-omi Yamaguchi, Katsunori Fujikura, Yosuke Miyairi, Yusuke Yokoyama, Toshi Nagata, Naohiko Ohkouchi
    • Journal Title

      Frontiers in Marine Science

      Volume: 8 Pages: 465-465

    • DOI

      10.3389/fmars.2021.641282

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Combined use of radiocarbon and stable carbon isotopes for the source mixing model in a stream food web2020

    • Author(s)
      Ishikawa Naoto F.、Finlay Jacques C.、Uno Hiromi、Ogawa Nanako O.、Ohkouchi Naohiko、Tayasu Ichiro、Power Mary E.
    • Journal Title

      Limnology and Oceanography

      Volume: 65 Issue: 11 Pages: 2688-2696

    • DOI

      10.1002/lno.11541

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Liquid Chromatographic Isolation of Individual Amino Acids Extracted From Sediments for Radiocarbon Analysis2020

    • Author(s)
      Blattmann Thomas M.、Montlucon Daniel B.、Haghipour Negar、Ishikawa Naoto F.、Eglinton Timothy I.
    • Journal Title

      Frontiers in Marine Science

      Volume: 7 Pages: 1-8

    • DOI

      10.3389/fmars.2020.00174

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A method for stable carbon isotope measurement of underivatized individual amino acids by multi‐dimensional high‐performance liquid chromatography and elemental analyzer/isotope ratio mass spectrometry2020

    • Author(s)
      Sun Yuchen、Ishikawa Naoto F.、Ogawa Nanako O.、Kawahata Hodaka、Takano Yoshinori、Ohkouchi Naohiko
    • Journal Title

      Rapid Communications in Mass Spectrometry

      Volume: 34 Issue: 20

    • DOI

      10.1002/rcm.8885

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Theoretical Amino Acid-Specific Radiocarbon Content in the Environment: Hypotheses to Be Tested and Opportunities to Be Taken2020

    • Author(s)
      Blattmann Thomas M.、Ishikawa Naoto F.
    • Journal Title

      Frontiers in Marine Science

      Volume: 7 Pages: 302-302

    • DOI

      10.3389/fmars.2020.00302

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Tracking long-distance migration of marine fishes using compound-specific stable isotope analysis of amino acids2020

    • Author(s)
      Matsubayashi J., Osada Y., Tadokoro K., Abe Y., Yamaguchi A., Shirai K., Honda K., Yoshikawa C., Ogawa N. O., Ohkouchi N., Ishikawa N. F., Nagata T., Miyamoto H., Nishino S., Tayasu I.
    • Journal Title

      Ecology Letters

      Volume: なし Issue: 5 Pages: 881-890

    • DOI

      10.1111/ele.13496

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Assessing alpha and beta diversities of benthic macroinvertebrates and their environmental drivers between watersheds with different levels of habitat transformation in Japan2019

    • Author(s)
      Ko Chia-Ying、Iwata Tomoya、Lee Jun-Yi、Murakami Aya、Okano Junichi、Ishikawa Naoto F.、Sakai Yoichiro、Tayasu Ichiro、Itoh Masayuki、Song Uhram、Togashi Hiroyuki、Nakano Shinich、Ohte Nobuhito、Okuda Noboru
    • Journal Title

      Marine and Freshwater Research

      Volume: 70 Issue: 4 Pages: 504-504

    • DOI

      10.1071/mf18031

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Compound-Specific Radiocarbon Analysis by Elemental Analyzer?Accelerator Mass Spectrometry: Precision and Limitations2019

    • Author(s)
      Haghipour N.、Ausin B.、Usman M. O.、Ishikawa N.、Wacker L.、Welte C.、Ueda K.、Eglinton T. I.
    • Journal Title

      Analytical Chemistry

      Volume: 91 Issue: 3 Pages: 2042-2049

    • DOI

      10.1021/acs.analchem.8b04491

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Radiocarbon age of different photoreactive fractions of freshwater dissolved organic matter2019

    • Author(s)
      Ishikawa Naoto F.、Butman David、Raymond Peter A.
    • Journal Title

      Organic Geochemistry

      Volume: 135 Pages: 11-15

    • DOI

      10.1016/j.orggeochem.2019.06.006

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Biogeochemical tools for characterizing organic carbon in inland aquatic ecosystems2018

    • Author(s)
      McCallister S. L.、Ishikawa N. F.、Kothawala D. N.
    • Journal Title

      Limnology and Oceanography Letters

      Volume: 3 Issue: 6 Pages: 444-457

    • DOI

      10.1002/lol2.10097

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Using food network unfolding to evaluate food-web complexity in terms of biodiversity: theory and applications2018

    • Author(s)
      Kato Yoshikazu、Kondoh Michio、Ishikawa Naoto F.、Togashi Hiroyuki、Kohmatsu Yukihiro、Yoshimura Mayumi、Yoshimizu Chikage、Haraguchi Takashi F.、Osada Yutaka、Ohte Nobuhito、Tokuchi Naoko、Okuda Noboru、Miki Takeshi、Tayasu Ichiro
    • Journal Title

      Ecology Letters

      Volume: 21 Issue: 7 Pages: 1065-1074

    • DOI

      10.1111/ele.12973

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improved Method for Isolation and Purification of Underivatized Amino Acids for Radiocarbon Analysis2018

    • Author(s)
      Ishikawa Naoto F.、Itahashi Yu、Blattmann Thomas M.、Takano Yoshinori、Ogawa Nanako O.、Yamane Masako、Yokoyama Yusuke、Nagata Toshi、Yoneda Minoru、Haghipour Negar、Eglinton Timothy I.、Ohkouchi Naohiko
    • Journal Title

      Analytical Chemistry

      Volume: 90 Issue: 20 Pages: 12035-12041

    • DOI

      10.1021/acs.analchem.8b02693

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A new analytical method for determination of the nitrogen isotopic composition of methionine: Its application to aquatic ecosystems with mixed resources.2018

    • Author(s)
      Ishikawa NF, Chikaraishi Y, Takano Y, Sasaki Y, Takizawa Y, Tsuchiya M, Tayasu I, Nagata T, Ohkouchi N
    • Journal Title

      Limnology & Oceanography: Methods

      Volume: 16 Issue: 9 Pages: 607-620

    • DOI

      10.1002/lom3.10272

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Use of compound-specific nitrogen isotope analysis of amino acids in trophic ecology: assumptions, applications, and implications2018

    • Author(s)
      Ishikawa Naoto F.
    • Journal Title

      Ecological Research

      Volume: 33 Issue: 5 Pages: 825-837

    • DOI

      10.1007/s11284-018-1616-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Global dataset for carbon and nitrogen stable isotope ratios of lotic periphyton2018

    • Author(s)
      Ishikawa Naoto F.、Doi Hideyuki、Finlay Jacques C.
    • Journal Title

      Ecological Research

      Volume: 33 Issue: 6 Pages: 1089-1089

    • DOI

      10.1007/s11284-018-1623-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Biomass pyramids of marine zooplankton communities as inferred from their integrated trophic positions2021

    • Author(s)
      *Naoto F. ISHIKAWA(JAMSTEC), Kazuaki TADOKORO(FRA), Jun MATSUBAYASHI(Chuo University), Naohiko OHKOUCHI(JAMSTEC)
    • Organizer
      The 68th Annual Meeting of Ecological Society of Japan (ESJ68)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Analysis of coastal marine ecosystems using radiocarbon and amino acid nitrogen isotopic compositions2020

    • Author(s)
      Ishikawa Naoto F., Chikaraishi Yoshito, Ogawa Nanako O., Miyairi Yosuke, Yokoyama Yusuke, Nagata Toshi, Ohkouchi Naohiko
    • Organizer
      The 67th Annual Meeting of Ecological Society of Japan (ESJ67)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Study of aquatic food web based on high-precision stable carbon isotopic measurement of individual amino acids2020

    • Author(s)
      Sun Yuchen, Ishikawa Naoto F., Kawahata Hodaka, Ogawa Nanako O., Takano Yoshinori, Ohkouchi Naohiko
    • Organizer
      The 67th Annual Meeting of Ecological Society of Japan (ESJ67)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Use of radiocarbon and compound-specific isotope analyses for ecological research2019

    • Author(s)
      Ishikawa Naoto F.
    • Organizer
      International Symposium on Isotope Physiology, Ecology, and Geochemistry 2019 (IsoPEG’19)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] アミノ酸の放射性炭素濃度測定法の開発と生物地球化学研究への応用2019

    • Author(s)
      石川尚人, 高野淑識, 小川奈々子, 大河内直彦
    • Organizer
      日本生態学会第66回全国大会
    • Related Report
      2018 Annual Research Report
  • [Book] 同位体環境学がえがく世界:2020 年版2020

    • Author(s)
      陀安一郎、申基澈、藤吉麗 編
    • Total Pages
      104
    • Publisher
      総合地球環境学研究所
    • ISBN
      9784906888764
    • Related Report
      2018 Annual Research Report
  • [Book] 河川生態系の調査・分析方法2019

    • Author(s)
      井上 幹生、中村 太士・編
    • Total Pages
      448
    • Publisher
      講談社
    • ISBN
      9784065169995
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-08-22  

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