| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
We systematically examined Sept3-deficient mice at various levels and the following findings were obtained. In systematic behavioral analysis, they selectively showed underperformance in the spatial context discrimination task. We pinpointed the responsible region by stereotactic injection of AAV expression and knockdown vectors. 3D morphometry using ssTEM revealed normal synaptic distribution density and spine volume, but fewer spines containing smooth endoplasmic reticulum. This abnormality was reproduced in primary cultured granule cells. We optimized condition of late long-term potentiation under which ER extension followed spine enlargement. SEPT3 depletion did not affect actin-based spine enlargement, while selectively inhibited ER extension, suggesting that ER entry into the granule cell spine is activity-dependent and SEPT3-dependent.
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