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High spatiotemporal resolution analyses of dynamics of synaptic vesicle proteins

Research Project

Project/Area Number 18H02526
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 46010:Neuroscience-general-related
Research InstitutionKyoto University

Principal Investigator

Hirano Tomoo  京都大学, 理学研究科, 教授 (50181178)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywordsシナプス / 神経伝達物質 / エキソサイトーシス / エンドサイトーシス / ライブイメージング / 蛍光タンパク質 / 脳・神経 / 蛍光ライブイメージング / 神経科学 / 生理学
Outline of Final Research Achievements

Exocytosis and endocytosis of a synaptic vesicle protein in and around a presynaptic active zone were studied by live-cell fluorescence imaging of synaptophysin (syp) tagged with a pH-sensitive fluorescent protein super-ecliptic phluorin (SEP) using a novel visualization technique. Syp-SEP which had moved to the cell membrane diffused after the exocytosis. Synchronous exocytosis occurring immediately after the electrical stimulation took place at several sites within an active zone, whereas asynchronous exocytosis occurring tens to hundreds milliseconds after the stimulation took a place at different sites. We also recorded endocytosis of syp-SEP occurring in the periphery of active zone. The endocytosis occurring within a second after the stimulation and that occurring later showed different characteristics such as temperature-dependence.

Academic Significance and Societal Importance of the Research Achievements

本研究では、未解明の問題が多く残されているシナプス前部からのシナプス小胞の開口放出と、シナプス小胞膜タンパク質の細胞内への回収過程の詳細を明らかにすることに寄与する実験方法を確立し、それを活用することにより新知見を得た。これらの研究成果は、脳・神経系における情報伝達の分子・細胞機構の解明に貢献するとともに、将来的には幾多の神経・精神疾患の発症メカニズムの理解向上を介して、それらの予防・治療方法の改善に寄与することが期待できる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (22 results)

All 2020 2019 2018 Other

All Journal Article (10 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 9 results,  Open Access: 5 results) Presentation (9 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Norepinephrine facilitates induction of long-term depression through β-adrenergic receptor at parallel fiber-to-Purkinje cell synapses in the flocculus.2020

    • Author(s)
      Inoshita T, Hirano T.
    • Journal Title

      Neuroscience

      Volume: - Pages: 141-150

    • DOI

      10.1016/j.neuroscience.2020.05.037

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Amyloid-β oligomers suppress subunit-specific glutamate receptor increase during LTP2019

    • Author(s)
      Hiroshi Tanaka, Daiki Sakaguchi, Tomoo Hirano
    • Journal Title

      Alzheimer’s & Dementia: Translational Research & Clinical Interventions

      Volume: 5 Issue: 1 Pages: 797-808

    • DOI

      10.1016/j.trci.2019.10.003

    • NAID

      120007006206

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] LTDとrebound potentiationのOKR制御における役割2019

    • Author(s)
      井下拓真、平野丈夫
    • Journal Title

      Clinical Neuroscience

      Volume: 37 Pages: 940-942

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Visualization of Synchronous or Asynchronous Release of Single Synaptic Vesicle in Active-Zone-Like Membrane Formed on Neuroligin-Coated Glass Surface2018

    • Author(s)
      Junichiro Funahashi, Hiromitsu Tanaka, Tomoo Hirano
    • Journal Title

      Frontiers in Cellular Neuroscience

      Volume: 12 Pages: 1-10

    • DOI

      10.3389/fncel.2018.00140

    • NAID

      120006478048

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Suppression of AMPA Receptor Exocytosis Contributes to Hippocampal LTD2018

    • Author(s)
      Shumpei Fujii, Hiromitsu Tanaka, Tomoo Hirano
    • Journal Title

      Journal of Neuroscience

      Volume: 38 Issue: 24 Pages: 5523-5537

    • DOI

      10.1523/jneurosci.3210-17.2018

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Synaptic m6A Epitranscriptome Reveals Functional Partitioning of Localized Transcripts for Dynamic Tripartite Synapse Modulation.2018

    • Author(s)
      Merkurjev, D., Hong, W. T., Iida, K., Oomoto, I., Goldie, B. J., Yamaguti, H., Ohara, T. Kawaguchi, S. Y., Hirano, T., Martin, K. C., Pellegrini, M. & Wang, D. O.
    • Journal Title

      Nature Neuroscience

      Volume: 21 Issue: 7 Pages: 1004-1014

    • DOI

      10.1038/s41593-018-0173-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ulk2 controls cortical excitatory?inhibitory balance via autophagic regulation of p62 and GABAA receptor trafficking in pyramidal neurons2018

    • Author(s)
      Sumitomo Akiko、Yukitake Hiroshi、Hirai Kazuko、Horike Kouta、Ueta Keisho、Chung Youjin、Warabi Eiji、Yanagawa Toru、Kitaoka Shiho、Furuyashiki Tomoyuki、Narumiya Shuh、Hirano Tomoo、Niwa Minae、Sibille Etienne、Hikida Takatoshi、Sakurai Takeshi、Ishizuka Koko、Sawa Akira、Tomoda Toshifumi
    • Journal Title

      Human Molecular Genetics

      Volume: 27 Issue: 18 Pages: 3165-3176

    • DOI

      10.1093/hmg/ddy219

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Visualization of exo- and endocytosis of AMPA receptors during hippocampal synaptic plasticity around postsynaptic-like membrane formed on glass surface.2018

    • Author(s)
      Hirano, T.
    • Journal Title

      Frontiers in Cellular Neuroscience.

      Volume: 12 Pages: 1-10

    • DOI

      10.3389/fncel.2018.00442

    • NAID

      120006547374

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regulation and interaction of multiple types of synaptic plasticity in a Purkinje neuron and their contribution to motor learning.2018

    • Author(s)
      Hirano,T.
    • Journal Title

      Cerebellum

      Volume: 17 Issue: 6 Pages: 756-765

    • DOI

      10.1007/s12311-018-0963-0

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purkinje Neurons: Development, Morphology and Function.2018

    • Author(s)
      Hirano,T.
    • Journal Title

      Cerebellum

      Volume: 17 Issue: 6 Pages: 699-700

    • DOI

      10.1007/s12311-018-0985-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] 膜電位感受性蛍光プローブを用いた神経活動の時空間解析2020

    • Author(s)
      森田雅登、平野丈夫、川口真也
    • Organizer
      第72回日本細胞生物学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Spatio-temporal integration of synaptic inputs in hippocampal neurons studied by membrane potential imaging2020

    • Author(s)
      森田雅登、平野丈夫、川口真也
    • Organizer
      第43回日本神経科学大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 海馬神経細胞におけるシナプス入力統合の膜電位イメージングによる時空間解析2020

    • Author(s)
      森田雅登、平野丈夫、川口真也
    • Organizer
      第97回日本生理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Effects of β-adrenergic receptor on long-term depression at parallel fiber to Purkinje neuron synapses in the cerebellar flocculus2019

    • Author(s)
      Inoshita T, Hirano T.
    • Organizer
      Neuroscience 2019, Chicago USA
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Different effects of noradrenaline on synaptic transmission and long-term depression at parallel fiber to Purkinje neuron synapses among cerebellar areas2019

    • Author(s)
      Inoshita T, Hirano T.
    • Organizer
      第42回 日本神経科学大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Contribution of excitatory and inhibitory synaptic plasticity in a Purkinje neuron to oculomotor learning paradigms2018

    • Author(s)
      Hirano, T.
    • Organizer
      75th Fujihara Seminar “Cerebellum as a CNS hub
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Subunit-specific effects on AMPA-type glutamate receptors caused by amyloid beta oligomers during hippocampal long-term potentiation2018

    • Author(s)
      Tanaka, H., Sakaguchi, D. & Hirano, T.
    • Organizer
      Neuroscience 2018 in USA
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アミロイドベータによるサブユニット特異的なグルタミン酸受容体の動態異常2018

    • Author(s)
      田中 洋光, 坂口 大輝, 平野 丈夫
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Regulation of reflex eye movement and its adaptation by norepinephrine in the cerebellar flocculus.2018

    • Author(s)
      Inoshita, T. & Hirano, T.
    • Organizer
      第41回 日本神経科学大会
    • Related Report
      2018 Annual Research Report
  • [Book] 何のための脳? AI時代の行動選択と神経科学2019

    • Author(s)
      平野丈夫
    • Total Pages
      145
    • Publisher
      京都大学学術出版会
    • ISBN
      9784814002306
    • Related Report
      2019 Annual Research Report
  • [Remarks] 京都大学大学院理学研究科生物科学専攻生物物理学教室神経生物学分科

    • URL

      http://www.nb.biophys.kyoto-u.ac.jp/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 平野研究室

    • URL

      http://www.neurosci.biophys.kyoto-u.ac.jp/main.html

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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