| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Outline of Final Research Achievements |
In order to understand the cellular mechanisms of transmission and plasticity at mammalian central synapses, we have applied simultaneous pre- and postsynaptic patch clamp to hippocampal mossy fiber synapses. We found that the intra-cellular Ca sensitivity for transmitter release was similar to the one at other synapse types, but the local Ca concentration at the transmitter release site was much lower, explaining low release probability of release at mossy fiber synapses. In addition, we found that cAMP-induced potentiation of transmitter release, important for short- and long-term plasticity, was mediated by accumulation of Ca channels near release sites, thereby increasing the local Ca concentration at the release site.
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