Generation of comparative connectomics
| Project/Area Number |
18H02548
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 46030:Function of nervous system-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Miyamichi Kazunari 国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (30612577)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Keywords | トランスシナプス標識 / 視床下部 / オキシトシン / 妊娠・出産 / 養育行動 / ウイルス遺伝子工学 / コネクトーム / CRIPSR / AAV / ミニプロモータ / アデノ随伴ウイルス / ゲノム編集 / CRISPR |
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| Outline of Final Research Achievements |
We aim to form a research field that we name ‘comparative connectomics’ to extract functionally important circuit elements by comparing the neural connection diagrams at a cell-type resolution based on rabies virus-mediated transsynaptic labeling method. Specifically, we examined input neural circuits to hypothalamic oxytocin neurons that secrete the hormone oxytocin in various reproductive states of mice and identified characteristic circuit alterations during pregnancy and lactation in female mice. Also, we established viral-genetic access to oxytocin neurons in ferrets as a model of Carnivora species.
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| Academic Significance and Societal Importance of the Research Achievements |
シナプス・スパイン動態の研究からミクロ (sub-μm) スケールの神経可塑性については特に記憶・学習の文脈で膨大な知見があるが、領域間をつなぐようなマクロスケール (supra-cm) の大域的な神経回路は発達後には不変と見なすのがカハール以来の伝統だった。本研究は成熟した哺乳類の神経回路がマクロスケールで再編成しうることを明らかにし、シナプス前後の神経細胞の種類を特定することに成功した点でパラダイムの転換を含む。これは将来的に脳機能の制御や失われた脳機能を回復させるための新たなイノベーションの基盤となり得る。
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] GABAergic neurons in the olfactory cortex projecting to the lateral hypothalamus in mice2019
Author(s)
Murata K, Kinoshita T, Fukazawa Y, Kobayashi K, Kobayashi K, Miyamichi K, Okuno H, Bito H, Sakurai Y, Yamaguchi M, Mori K, Manabe H
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Journal Title
Sci Rep
Volume: 9
Issue: 1
Pages: 7132-7132
DOI
NAID
Related Report
Peer Reviewed / Open Access
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